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protein coding gene - csm1 (SPAC11E3.03) - microtubule-site clamp monopolin complex subunit Csm1/Pcs1

Gene summary

Standard name
csm1
Systematic ID
SPAC11E3.03
Product
microtubule-site clamp monopolin complex subunit Csm1/Pcs1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pcs1
UniProt ID
O13684
ORFeome ID
12/12F02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5286110..5287290 forward strand

Annotation

GO biological process

GO:0051315 - attachment of mitotic spindle microtubules to kinetochore

References:

GO:0045144 - meiotic sister chromatid segregation

References:

GO:1990893 - mitotic chromosome centromere condensation

References:

GO:0000070 - mitotic sister chromatid segregation

References:

GO cellular component

GO:0034506 - chromosome, centromeric core domain

References:

GO:0000775 - chromosome, centromeric region

References:

GO:0005829 - cytosol

References:

GO:0000776 - kinetochore

References:

GO:0072686 - mitotic spindle

References:

GO:0033551 - monopolin complex

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:1990644 - microtubule site clamp

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0002909 - decreased protein localization to chromatin during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000316 - inviable after spore germination

References:

Genotypes:

FYPO:0002150 - inviable spore population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000449 - abnormal protein localization to centromere during vegetative growth

References:

Genotypes:

FYPO:0001946 - abolished mitotic sister chromatid separation

References:

Genotypes:

FYPO:0001270 - complete but unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0004904 - decreased protein localization to centromere central core during vegetative growth

References:

Genotypes:

FYPO:0002391 - decreased protein localization to chromatin at rDNA

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002636 - delayed onset of mitotic spindle elongation

References:

Genotypes:

FYPO:0002649 - elongated kinetochore during mitosis

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0004393 - lagging chromosomes during meiosis I

References:

Genotypes:

FYPO:0004394 - lagging chromosomes during meiosis II

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0004101 - lagging mitotic chromosomes, with complete sister chromatid separation

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0002651 - merotelic kinetochore attachment

References:

Genotypes:

FYPO:0004381 - merotelic kinetochore attachment during mitosis

References:

Genotypes:

FYPO:0004382 - meroterically attached lagging mitotic chromosomes

References:

Genotypes:

FYPO:0004331 - normal chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003075 - normal protein kinase activity

References:

Genotypes:

FYPO:0002574 - normal protein localization to centromere during vegetative growth

References:

Genotypes:

FYPO:0001509 - normal protein localization to chromatin during vegetative growth

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0005968 - resistance to sodium chloride

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0003182 - sister chromatid nondisjunction at meiosis II

References:

Genotypes:

FYPO:0002650 - split kinetochore during vegetative growth

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF12539Csm1-Csm1/Pcs1_CPfam
cd23787RWD_CSM1CDD
G3DSA:3.90.1150.80Csm1/Pcs1_C_sfCATH-Gene3D
PTHR28006Csm1/Pcs1PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:17627824 - The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation.
Gregan J et al. Curr Biol 2007 Jul 17;17(14):1190-200
PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.
Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:20937798 - Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance.
Mendoza-Cózatl DG et al. J Biol Chem 2010 Dec 24;285(52):40416-26
PMID:12689592 - Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I.
Rabitsch KP et al. Dev Cell 2003 Apr;4(4):535-48
PMID:20723757 - The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments.
Corbett KD et al. Cell 2010 Aug 20;142(4):556-67
PMID:31037469 - The molecular basis of monopolin recruitment to the kinetochore.
Plowman R et al. Chromosoma 2019 Sep;128(3):331-354
PMID:19758558 - CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis.
Tanaka K et al. Dev Cell 2009 Sep;17(3):334-43
PMID:20221439 - Detoxification of multiple heavy metals by a half-molecule ABC transporter, HMT-1, and coelomocytes of Caenorhabditis elegans.
Schwartz MS et al. PLoS One 2010 Mar 05;5(3):e9564
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:20935472 - Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4.
Rumpf C et al. Cell Cycle 2010 Oct 01;9(19):3997-4004
PMID:25778919 - Aurora B prevents chromosome arm separation defects by promoting telomere dispersion and disjunction.
Reyes C et al. J Cell Biol 2015 Mar 16;208(6):713-27
PMID:19523829 - Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation.
Choi SH et al. Curr Biol 2009 Jun 23;19(12):985-95
PMID:21633354 - Condensin association with histone H2A shapes mitotic chromosomes.
Tada K et al. Nature 2011 Jun 01;474(7352):477-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.
Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235