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protein coding gene - ubc13 (SPAC11E3.04c) - ubiquitin conjugating enzyme E2 Ubc13

Gene summary

Standard name
ubc13
Systematic ID
SPAC11E3.04c
Product
ubiquitin conjugating enzyme E2 Ubc13
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
spu13, sst5
UniProt ID
O13685
ORFeome ID
52/52A04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5287180..5288094 reverse strand

Annotation

GO biological process

GO:0120113 - cytoplasm to vacuole targeting by the NVT pathway

References:

GO:0006301 - DNA damage tolerance

References:

GO:0070914 - UV-damage excision repair

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0061631 - ubiquitin conjugating enzyme activity

References:

GO:0061630 - ubiquitin protein ligase activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0006274 - abolished protein localization via NVT pathway

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0006679 - normal growth on chromium

References:

Genotypes:

FYPO:0005680 - normal number of Rad52 foci during mitotic S phase

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0005324 - decreased error-free translesion synthesis of cyclobutane pyrimidine dimers

References:

Genotypes:

FYPO:0005330 - decreased error-free translesion synthesis of pyrimidine-pyrimidone 6-4 photoproducts

References:

Genotypes:

FYPO:0005331 - decreased error-prone translesion synthesis of pyrimidine-pyrimidone 6-4 photoproducts

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0006811 - normal gross chromosomal rearrangement frequency

References:

Genotypes:

FYPO:0001690 - normal growth on camptothecin

References:

Genotypes:

FYPO:0006679 - normal growth on chromium

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0005193 - resistance to torin1

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00179UQ_conUBCPFAM
cd23813UBCc_UBE2NCDD
PS00183UBC_1UBQ-conjugating_ASPROSITE_PATTERNS
PS50127UBC_2UBCPROSITE_PROFILES
SM00212ubc_7SMART
G3DSA:3.10.110.10:FF:000091FUNFAM
SSF54495UBC-likeUBQ-conjugating_enzyme/RWDSUPERFAMILY
G3DSA:3.10.110.10Ubiquitin Conjugating EnzymeUBQ-conjugating_enzyme/RWDGENE3D
PTHR24067UBIQUITIN-CONJUGATING ENZYME E2Ub_conjugating_enzyme-E2-likePANTHER

Orthologs

References / Literature

PMID:12951513 - Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in Schizosaccharomyces pombe.
Onishi M et al. Biosci Biotechnol Biochem 2003 Aug;67(8):1772-9
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24265825 - Concerted action of the ubiquitin-fusion degradation protein 1 (Ufd1) and Sumo-targeted ubiquitin ligases (STUbLs) in the DNA-damage response.
Køhler JB et al. PLoS One 2013;8(11):e80442
PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.
Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95
PMID:35011726 - Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase.
Kolesar P et al. Cells 2022 Jan 04;11(1)
PMID:28162934 - S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity.
Lv Z et al. Mol Cell 2017 Feb 16;65(4):699-714.e6
PMID:38718864 - H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.
Qin B et al. J Biol Chem 2024 May 06;:107345
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:26365378 - ESCRTs Cooperate with a Selective Autophagy Receptor to Mediate Vacuolar Targeting of Soluble Cargos.
Liu XM et al. Mol Cell 2015 Sep 17;59(6):1035-42
PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.
Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595
PMID:28718400 - Histone H3G34R mutation causes replication stress, homologous recombination defects and genomic instability in S. pombe .
Yadav RK et al. Elife 2017 Jul 18;6
PMID:39789818 - SRPKs Homolog Dsk1 Regulates Homologous Recombination Repair in Schizosaccharomyces pombe.
Lu G et al. Genes Cells 2025 Jan;30(1):e13192
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.
Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)
PMID:28481910 - PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.
Daigaku Y et al. PLoS Genet 2017 May;13(5):e1006789
PMID:36088506 - Mrz1, a Novel Mitochondrial Outer Membrane RING Finger Protein, is Degraded Through the Ubiquitin-Proteasome Pathway in Schizosaccharomyces pombe.
Liu Z et al. Curr Microbiol 2022 Sep 10;79(10):309
PMID:16641370 - Postreplication repair and PCNA modification in Schizosaccharomyces pombe.
Frampton J et al. Mol Biol Cell 2006 Jul;17(7):2976-85
PMID:12531016 - Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe.
Brown M et al. DNA Repair (Amst) 2002 Nov 03;1(11):869-80
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:35075549 - Schizosaccharomyces pombe Fzo1 is subjected to the ubiquitin-proteasome-mediated degradation during the stationary phase.
Ahmad F et al. Int Microbiol 2022 May;25(2):397-404
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:21247416 - Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast.
Gatti L et al. BMC Genomics 2011 Jan 19;12:44
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25293972 - Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).
Mastro TL et al. Genetics 2014 Dec;198(4):1457-72
PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.
Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.
Dolan WP et al. Genetics 2010 May;185(1):39-53
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23416107 - Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Olsen SK et al. Mol Cell 2013 Mar 07;49(5):884-96
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:27151298 - Orderly progression through S-phase requires dynamic ubiquitylation and deubiquitylation of PCNA.
Álvarez V et al. Sci Rep 2016 May 06;6:25513
PMID:34292936 - Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination.
Su J et al. PLoS Genet 2021 Jul;17(7):e1009671
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22064477 - The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast.
Groocock LM et al. Mol Cell Biol 2012 Jan;32(2):276-87
PMID:24687850 - H2A.Z-dependent regulation of cohesin dynamics on chromosome arms.
Tapia-Alveal C et al. Mol Cell Biol 2014 Jun;34(11):2092-104
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:20453833 - Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast.
Coulon S et al. EMBO J 2010 Jun 16;29(12):2048-58
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60