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protein coding gene - nse6 (SPAC11E3.08c) - Smc5-6 complex non-SMC subunit Nse6

Gene summary

Standard name
nse6
Systematic ID
SPAC11E3.08c
Product
Smc5-6 complex non-SMC subunit Nse6
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
cor1
UniProt ID
O13688
ORFeome ID
52/52B04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5296049..5297780 reverse strand

Annotation

Disease association

MONDO:0859575 - Atelis syndrome 1

References:

GO biological process

GO:0006325 - chromatin organization

References:

GO:0006974 - DNA damage response

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GO:0006302 - double-strand break repair

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GO:0000724 - double-strand break repair via homologous recombination

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GO cellular component

GO:0005634 - nucleus

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GO:0030915 - Smc5-Smc6 complex

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GO molecular function

GO:0140463 - chromatin-protein adaptor activity

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GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0002125 - abnormal protein localization to plasma membrane during vegetative growth

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0005018 - decreased double-stranded DNA binding

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Genotypes:

FYPO:0002775 - decreased level of sumoylated protein in cell

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0004909 - loss of punctate nuclear protein localization, with protein distributed in nucleus

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0091600 - cor-C? is nse6

References:

Protein features

IDNameInterPro nameDB name
PF08691Nse5DNA_repair_Nse5/Nse6Pfam
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
PMID:30600397 - Recruitment, loading, and activation of the Smc5-Smc6 SUMO ligase.
Oravcová M et al. Curr Genet 2019 Jun;65(3):669-676
PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.
Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6
PMID:32546830 - A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6.
Vondrova L et al. Sci Rep 2020 Jun 16;10(1):9694
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:17005570 - The Smc5-Smc6 DNA repair complex. bridging of the Smc5-Smc6 heads by the KLEISIN, Nse4, and non-Kleisin subunits.
Palecek J et al. J Biol Chem 2006 Dec 01;281(48):36952-9
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:35011726 - Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase.
Kolesar P et al. Cells 2022 Jan 04;11(1)
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:10574765 - Mis-specification of cortical identity in a fission yeast PAK mutant.
Sawin KE et al. Curr Biol 1999 Nov 18;9(22):1335-8
PMID:21965289 - Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.
Buttrick GJ et al. Mol Biol Cell 2011 Dec;22(23):4486-502
PMID:16478984 - The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.
Pebernard S et al. Mol Cell Biol 2006 Mar;26(5):1617-30
PMID:30348841 - Brc1 Promotes the Focal Accumulation and SUMO Ligase Activity of Smc5-Smc6 during Replication Stress.
Oravcová M et al. Mol Cell Biol 2019 Jan 15;39(2)
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:25002536 - A novel histone deacetylase complex in the control of transcription and genome stability.
Zilio N et al. Mol Cell Biol 2014 Sep 15;34(18):3500-14
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19363481 - Molecular mimicry of SUMO promotes DNA repair.
Prudden J et al. Nat Struct Mol Biol 2009 May;16(5):509-16
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:17762865 - SUMO-targeted ubiquitin ligases in genome stability.
Prudden J et al. EMBO J 2007 Sep 19;26(18):4089-101
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:22064477 - The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast.
Groocock LM et al. Mol Cell Biol 2012 Jan;32(2):276-87
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22855558 - Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex.
Wehrkamp-Richter S et al. Nucleic Acids Res 2012 Oct;40(19):9633-46
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38