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protein coding gene - arp2 (SPAC11H11.06) - ARP2/3 actin-organizing complex actin-related protein subunit Arp2

Gene summary

Standard name
arp2
Systematic ID
SPAC11H11.06
Product
ARP2/3 actin-organizing complex actin-related protein subunit Arp2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC22F8.01
UniProt ID
Q9UUJ1
ORFeome ID
19/19D11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4783022..4784765 forward strand

Annotation

GO biological process

GO:0090135 - actin filament branching

References:

GO:0034314 - Arp2/3 complex-mediated actin nucleation

References:

GO:0072583 - clathrin-dependent endocytosis

References:

GO:0045324 - late endosome to vacuole transport

References:

GO cellular component

GO:0030479 - actin cortical patch

References:

GO:0005885 - Arp2/3 protein complex

References:

GO:0051285 - cell cortex of cell tip

References:

GO:0031097 - medial cortex

References:

GO molecular function

GO:0051015 - actin filament binding

References:

GO:0005524 - ATP binding

References:

GO:0005200 - structural constituent of cytoskeleton

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0002150 - inviable spore population

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0007440 - abnormal intracellular sterol transport

References:

Genotypes:

FYPO:0006116 - abolished protein localization to actin cortical patch

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

References:

Genotypes:

FYPO:0007454 - decreased ATP binding

References:

Genotypes:

FYPO:0000422 - decreased endocytosis during vegetative growth

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0007453 - decreased protein level in Arp2/3 complex

References:

Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0004967 - increased vegetative cell population growth during phosphate starvation

References:

Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001367 - normal cytokinesis

References:

Genotypes:

FYPO:0007579 - normal medial membrane band sterol distribution

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00022ActinActinPFAM
cd10220ASKHA_NBD_Arp2CDD
PS01132ACTINS_ACT_LIKEActin/actin-like_CSPROSITE_PATTERNS
SM00268actin_3ActinSMART
PR00190ACTINActinPRINTS
G3DSA:3.30.420.40:FF:000148FUNFAM
G3DSA:3.90.640.10:FF:000005FUNFAM
SSF53067Actin-like ATPase domainATPase_NBDSUPERFAMILY
G3DSA:3.90.640.10Actin; Chain A, domain 4GENE3D
G3DSA:3.30.420.40GENE3D
PTHR11937ACTINActinPANTHER

Orthologs

References / Literature

PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.
Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.
Wu JQ et al. Science 2005 Oct 14;310(5746):310-4
PMID:18640983 - Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
Nolen BJ et al. J Biol Chem 2008 Sep 26;283(39):26490-8
PMID:20587778 - Quantitative analysis of the mechanism of endocytic actin patch assembly and disassembly in fission yeast.
Sirotkin V et al. Mol Biol Cell 2010 Aug 15;21(16):2894-904
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:32839613 - Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
Shaaban M et al. Nat Struct Mol Biol 2020 Nov;27(11):1009-1016
PMID:27417392 - Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.
Rodnick-Smith M et al. Nat Commun 2016 Jul 15;7:12226
PMID:24798735 - The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.
Wang N et al. J Cell Biol 2014 May 12;205(3):357-75
PMID:18165685 - Pathway of actin filament branch formation by Arp2/3 complex.
Beltzner CC et al. J Biol Chem 2008 Mar 14;283(11):7135-44
PMID:19362000 - Cofilin dissociates Arp2/3 complex and branches from actin filaments.
Chan C et al. Curr Biol 2009 Apr 14;19(7):537-45
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:15517003 - Organization of a sterol-rich membrane domain by cdc15p during cytokinesis in fission yeast.
Takeda T et al. Nat Cell Biol 2004 Nov;6(11):1142-4
PMID:41850284 - Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast.
Melero A et al. Curr Biol 2026 Mar 17;
PMID:20517925 - GMF is an evolutionarily developed Adf/cofilin-super family protein involved in the Arp2/3 complex-mediated organization of the actin cytoskeleton.
Nakano K et al. Cytoskeleton (Hoboken) 2010 Jun;67(6):373-82
PMID:38448439 - Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex.
Chavali SS et al. Nat Commun 2024 Mar 06;15(1):2059
PMID:30471998 - Dip1 Co-opts Features of Branching Nucleation to Create Linear Actin Filaments that Activate WASP-Bound Arp2/3 Complex.
Balzer CJ et al. Curr Biol 2018 Dec 03;28(23):3886-3891.e4
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:16087707 - Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
Sirotkin V et al. J Cell Biol 2005 Aug 15;170(4):637-48
PMID:26345368 - A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.
Petrini E et al. J Cell Sci 2015 Oct 15;128(20):3707-13
PMID:23148219 - Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.
Liu SL et al. J Biol Chem 2013 Jan 04;288(1):487-97
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30150414 - Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.
Espinoza-Sanchez S et al. Proc Natl Acad Sci U S A 2018 Sep 11;115(37):E8642-E8651
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:32320462 - Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
Marek M et al. J Cell Biol 2020 Jun 01;219(6)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25543282 - Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.
Suarez C et al. Dev Cell 2015 Jan 12;32(1):43-53
PMID:21642440 - Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast.
Skau CT et al. J Biol Chem 2011 Jul 29;286(30):26964-77
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:10588653 - A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast.
Morrell JL et al. Mol Biol Cell 1999 Dec;10(12):4201-15
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:21676862 - Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.
Ti SC et al. Proc Natl Acad Sci U S A 2011 Aug 16;108(33):E463-71
PMID:22144913 - Widespread cotranslational formation of protein complexes.
Duncan CD et al. PLoS Genet 2011 Dec;7(12):e1002398
PMID:36442092 - Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
Chou SZ et al. Proc Natl Acad Sci U S A 2022 Dec 06;119(49):e2206722119
PMID:39012625 - Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.
Harrell MA et al. J Cell Biol 2024 Sep 02;223(10)
PMID:16141239 - Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis.
Gachet Y et al. J Cell Sci 2005 Sep 15;118(Pt 18):4231-42