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protein coding gene - sxa2 (SPAC1296.03c) - serine carboxypeptidase Sxa2

Gene summary

Standard name
sxa2
Systematic ID
SPAC1296.03c
Product
serine carboxypeptidase Sxa2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P32825
ORFeome ID
24/24G01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 712217..714449 reverse strand

Annotation

GO biological process

GO:0000747 - conjugation with cellular fusion

References:

GO:0031138 - negative regulation of conjugation with cellular fusion

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GO:0010515 - negative regulation of induction of conjugation with cellular fusion

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GO:0043409 - negative regulation of MAPK cascade

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GO:0180040 - negative regulation of pheromone response MAPK cascade

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GO:0030163 - protein catabolic process

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GO cellular component

GO:0005829 - cytosol

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GO:0005576 - extracellular region

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GO:0005634 - nucleus

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GO molecular function

GO:0004180 - carboxypeptidase activity

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GO:0004185 - serine-type carboxypeptidase activity

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Modification

MOD:00006 - N-glycosylated residue

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MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0005256 - decreased carboxypeptidase activity

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Genotypes:

FYPO:0000668 - decreased peptidase activity

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Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

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Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

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Genotypes:

FYPO:0001115 - normal cellular response to P-factor

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Genotypes:

FYPO:0006573 - normal protein level during cellular response to pheromone

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Genotypes:

FYPO:0000573 - normal shmoo formation

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000031 - abnormal mating

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Genotypes:

FYPO:0000669 - abolished peptidase activity

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Genotypes:

FYPO:0001669 - abolished protein processing during vegetative growth

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Genotypes:

FYPO:0000537 - abolished protein secretion during vegetative growth

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Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

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Genotypes:

FYPO:0002520 - decreased frequency of conjugation with h+ cells

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Genotypes:

FYPO:0000668 - decreased peptidase activity

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Genotypes:

FYPO:0001871 - increased agglutination

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Genotypes:

FYPO:0000819 - increased concentration of P-factor in growth medium

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Genotypes:

FYPO:0000667 - increased peptidase activity

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Genotypes:

FYPO:0000572 - increased rate of shmoo formation

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Genotypes:

FYPO:0004581 - increased transcriptional response to pheromone

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Genotypes:

FYPO:0000665 - normal peptidase activity

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Genotypes:

FYPO:0001668 - normal protein processing during vegetative growth

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0000022 - shmoo with long tip

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011064 - conserved in fungi

PBO:0000110 - orthologs cannot be distinguished

Protein features

IDNameInterPro nameDB name
PF00450Peptidase_S10Peptidase_S10PFAM
PS00131CARBOXYPEPT_SER_SERSer_caboxypep_ser_ASPROSITE_PATTERNS
PS00560CARBOXYPEPT_SER_HISSer_caboxypep_his_ASPROSITE_PATTERNS
PR00724CRBOXYPTASECPeptidase_S10PRINTS
SSF53474alpha/beta-HydrolasesAB_hydrolase_foldSUPERFAMILY
G3DSA:3.40.50.1820alpha/beta hydrolaseAB_hydrolase_foldGENE3D
PTHR11802SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASEPeptidase_S10PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder

Orthologs

References / Literature

PMID:25803873 - Calnexin is essential for survival under nitrogen starvation and stationary phase in Schizosaccharomyces pombe.
Núñez A et al. PLoS One 2015;10(3):e0121059
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:31239353 - Zinc-dependent activation of the Pho8 alkaline phosphatase in Schizosaccharomyces pombe .
Hu YM et al. J Biol Chem 2019 Aug 16;294(33):12392-12404
PMID:9191273 - The role of Sxa1 in pheromone recovery in Schizosaccharomyces pombe.
Hughes M et al. Biochem Soc Trans 1997 May;25(2):229S
PMID:30279276 - Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.
Gallo Castro D et al. J Cell Biol 2018 Dec 03;217(12):4215-4229
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:10792724 - Sxa2 is a serine carboxypeptidase that degrades extracellular P-factor in the fission yeast Schizosaccharomyces pombe.
Ladds G et al. Mol Microbiol 2000 Apr;36(2):377-90
PMID:7499258 - Cloning and functional analysis of the ndk1 gene encoding nucleoside-diphosphate kinase in Schizosaccharomyces pombe.
Izumiya H et al. J Biol Chem 1995 Nov 17;270(46):27859-64
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:8668157 - Schizosaccharomyces pombe map1+ encodes a MADS-box-family protein required for cell-type-specific gene expression.
Yabana N et al. Mol Cell Biol 1996 Jul;16(7):3420-8
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:8736868 - Characterisation of Sxa2, a carboxypeptidase involved in pheromone recovery in fission yeast.
Ladds G et al. Biochem Soc Trans 1996 May;24(2):210S
PMID:9671458 - An RNA binding protein negatively controlling differentiation in fission yeast.
Tsukahara K et al. Mol Cell Biol 1998 Aug;18(8):4488-98
PMID:8654750 - Characterisation of Sxa2, a protease involved in pheromone communication in fission yeast.
Ladds G et al. Biochem Soc Trans 1995 Nov;23(4):565S
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:21153812 - Processing and maturation of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe.
Mukaiyama H et al. Appl Microbiol Biotechnol 2011 Apr;90(1):203-13
PMID:1549128 - Schizosaccharomyces pombe sxa1+ and sxa2+ encode putative proteases involved in the mating response.
Imai Y et al. Mol Cell Biol 1992 Apr;12(4):1827-34
PMID:11554925 - The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating.
Pereira PS et al. Genes Cells 2001 Sep;6(9):789-802
PMID:8861202 - The sxa2-dependent inactivation of the P-factor mating pheromone in the fission yeast Schizosaccharomyces pombe.
Ladds G et al. Mol Microbiol 1996 Apr;20(1):35-42
PMID:9599405 - Extracellular degradation of agonists as an adaptive mechanism.
Ladds G et al. Semin Cell Dev Biol 1998 Apr;9(2):111-8
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:7900424 - Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation.
Egel R et al. Yeast 1994 Oct;10(10):1347-54
PMID:27020743 - Local Pheromone Release from Dynamic Polarity Sites Underlies Cell-Cell Pairing during Yeast Mating.
Merlini L et al. Curr Biol 2016 Apr 25;26(8):1117-25
PMID:9191272 - Sxa2, a carboxypeptidase that degrades extracellular pheromone in fission yeast.
Ladds G et al. Biochem Soc Trans 1997 May;25(2):228S
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11950884 - Different mechanisms of cell polarisation in vegetative and shmooing growth in fission yeast.
Niccoli T et al. J Cell Sci 2002 Apr 15;115(Pt 8):1651-62
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:8879048 - Purification of Sxa2, a carboxypeptidase involved in pheromone recovery in fission yeast.
Ladds G et al. Biochem Soc Trans 1996 Aug;24(3):504S
PMID:19669754 - Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway.
Idiris A et al. Appl Microbiol Biotechnol 2010 Jan;85(3):667-77
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
PMID:27022830 - Microscopy of Fission Yeast Sexual Lifecycle.
Vjestica A et al. J Vis Exp 2016 Mar 09;(109)
PMID:16802154 - Enhanced productivity of protease-sensitive heterologous proteins by disruption of multiple protease genes in the fission yeast Schizosaccharomyces pombe.
Idiris A et al. Appl Microbiol Biotechnol 2006 Nov;73(2):404-20
PMID:8314086 - The fission yeast mating pheromone P-factor: its molecular structure, gene structure, and ability to induce gene expression and G1 arrest in the mating partner.
Imai Y et al. Genes Dev 1994 Feb 01;8(3):328-38
PMID:24147005 - The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.
Weston C et al. PLoS One 2013;8(10):e77487