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protein coding gene - exg2 (SPAC12B10.11) - cell wall glucan glucosidase Exg2

Gene summary

Standard name
exg2
Systematic ID
SPAC12B10.11
Product
cell wall glucan glucosidase Exg2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10444
ORFeome ID
49/49B07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4591855..4593567 forward strand

Annotation

GO biological process

GO:0031505 - fungal-type cell wall organization

References:

GO:1904541 - mating projection tip cell wall disassembly

References:

GO cellular component

GO:0000935 - division septum

References:

GO:1990819 - mating projection actin fusion focus

References:

GO:0016020 - membrane

References:

GO:0005886 - plasma membrane

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GO:0031520 - plasma membrane of cell tip

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GO molecular function

GO:0046557 - glucan endo-1,6-beta-glucosidase activity

References:

Modification

MOD:00689 - disulfide crosslinked residues

References:

MOD:00006 - N-glycosylated residue

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Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000182 - abnormal cell wall organization during vegetative growth

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001084 - increased cell wall alpha-glucan level

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Genotypes:

FYPO:0004860 - increased cell wall beta-glucan level

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Genotypes:

FYPO:0001035 - increased cell wall thickness during vegetative growth

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Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002107 - inviable stubby vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

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Genotypes:

FYPO:0004861 - normal cell wall galactomannan level

References:

Genotypes:

FYPO:0002161 - normal growth on Calcofluor White

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Genotypes:

FYPO:0004858 - normal growth on Congo Red

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
G3DSA:3.20.20.80:FF:000033CATH-FunFam
G3DSA:3.20.20.80CATH-Gene3D
SSF51445GH_hydrolase_sfSUPERFAMILY
PTHR31297Glycosyl_hydrolase_5PANTHER
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25825517 - A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
Dudin O et al. J Cell Biol 2015 Mar 30;208(7):897-911
PMID:20852022 - Characterization of glycoside hydrolase family 5 proteins in Schizosaccharomyces pombe.
Dueñas-Santero E et al. Eukaryot Cell 2010 Nov;9(11):1650-60
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963