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protein coding gene - tht1 (SPAC13C5.03) - nuclear membrane protein involved in karyogamy Tht1

Gene summary

Standard name
tht1
Systematic ID
SPAC13C5.03
Product
nuclear membrane protein involved in karyogamy Tht1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09684
ORFeome ID
35/35G11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 427473..430121 forward strand

Annotation

GO biological process

GO:0000742 - karyogamy involved in conjugation with cellular fusion

References:

GO:0048288 - nuclear membrane fusion involved in karyogamy

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GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005789 - endoplasmic reticulum membrane

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GO:0031965 - nuclear membrane

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GO:0005640 - nuclear outer membrane

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Modification

MOD:00693 - glycosylated residue

References:

MOD:00115 - S-palmitoyl-L-cysteine

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000022 - protein absent

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PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000015 - RNA absent

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002890 - abnormal horsetail nucleus morphology

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Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0000510 - abnormal nuclear fusion during mating

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Genotypes:

FYPO:0002711 - abnormal nuclear separation after meiosis

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Genotypes:

FYPO:0000348 - abnormal spore morphology

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Genotypes:

FYPO:0000913 - abnormal sporulation resulting in formation of ascus containing non-uniform spores

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Genotypes:

FYPO:0000681 - abnormal sporulation resulting in formation of two-spore ascus

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Genotypes:

FYPO:0000511 - abolished nuclear fusion during mating

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0001911 - decreased protein glycosylation during vegetative growth

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Genotypes:

FYPO:0003967 - decreased protein level in stationary phase

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0001386 - increased haploidization

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Genotypes:

FYPO:0003061 - meiotic cell cycle in zygote with unfused nuclei

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Genotypes:

FYPO:0001909 - normal protein glycosylation during vegetative growth

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0003182 - sister chromatid nondisjunction at meiosis II

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Genotypes:

FYPO:0000346 - small spores

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04163Tht1Nuclear_fusion_Kar5Pfam
PTHR28012Nuclear_fusion_Kar5PANTHER
Transmembrane alpha helixDeepTMHMM
CoilCOILS

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:36650056 - Protein S-palmitoylation regulates different stages of meiosis in Schizosaccharomyces pombe .
Pham TV et al. Life Sci Alliance 2023 Apr;6(4)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:9442101 - A novel fission yeast gene, tht1+, is required for the fusion of nuclear envelopes during karyogamy.
Tange Y et al. J Cell Biol 1998 Jan 26;140(2):247-58
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053