protein coding gene - rad8 (SPAC13G6.01c) - ATP-dependent chromatin remodeller/ubiquitin-protein ligase E3 Rad8

Gene summary

Standard name

rad8

Systematic ID

SPAC13G6.01c

Product

ATP-dependent chromatin remodeller/ubiquitin-protein ligase E3 Rad8

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

SPAC5H10.14c

UniProt ID

P36607

ORFeome ID

48/48C04

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 170489..174692 reverse strand

Annotation

Protein features

Orthologs

• HLTF (HGNC:11099) Homo sapiens
• rad8 (SJAG_02521) Schizosaccharomyces japonicus
• RAD5 (YLR032W) Saccharomyces cerevisiae

References / Literature

PMID:41330900 - PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast.

Markowska K et al. Nat Commun 2025 Dec 02;16(1):10837

PMID:9154834 - Discrete roles of the Spc1 kinase and the Atf1 transcription factor in the UV response of Schizosaccharomyces pombe.

Degols G et al. Mol Cell Biol 1997 Jun;17(6):3356-63

PMID:8709952 - The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe.

Hegde V et al. Mol Gen Genet 1996 Jun 24;251(4):483-92

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:26652183 - Coordination of DNA damage tolerance mechanisms with cell cycle progression in fission yeast.

Callegari AJ et al. Cell Cycle 2016;15(2):261-73

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:20452294 - Ubiquitin-PCNA fusion as a mimic for mono-ubiquitinated PCNA in Schizosaccharomyces pombe.

Ramasubramanyan S et al. DNA Repair (Amst) 2010 Jul 01;9(7):777-84

PMID:25313826 - The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.

Pietrobon V et al. PLoS Biol 2014 Oct;12(10):e1001968

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:34292936 - Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination.

Su J et al. PLoS Genet 2021 Jul;17(7):e1009671

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:16641370 - Postreplication repair and PCNA modification in Schizosaccharomyces pombe.

Frampton J et al. Mol Biol Cell 2006 Jul;17(7):2976-85

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

GO_REF:0000033 - Annotation inferences using phylogenetic trees

GO_REF:0000002 - Comments

PMID:20453833 - Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast.

Coulon S et al. EMBO J 2010 Jun 16;29(12):2048-58

PMID:9487130 - Regulation of telomere length by checkpoint genes in Schizosaccharomyces pombe.

Dahlen M et al. Mol Biol Cell 1998 Mar;9(3):611-21

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:9658208 - Sensitivity to cisplatin and platinum-containing compounds of Schizosaccharomyces pombe rad mutants.

Perego P et al. Mol Pharmacol 1998 Jul;54(1):213-9

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:27151298 - Orderly progression through S-phase requires dynamic ubiquitylation and deubiquitylation of PCNA.

Álvarez V et al. Sci Rep 2016 May 06;6:25513

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:8290359 - Cloning and characterisation of the Schizosaccharomyces pombe rad8 gene, a member of the SNF2 helicase family.

Doe CL et al. Nucleic Acids Res 1993 Dec 25;21(25):5964-71

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:9092625 - Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.

Shayeghi M et al. Nucleic Acids Res 1997 Mar 15;25(6):1162-9

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:7254221 - Spontaneous and UV-induced recombination in radiation-sensitive mutants of Schizosaccharomyces pombe.

Grossenbacher-Grunder AM et al. Mutat Res 1981 Mar;81(1):37-48

PMID:21493688 - Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.

Guarino E et al. Nucleic Acids Res 2011 Aug;39(14):5978-90

PMID:14993467 - The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.

Doe CL et al. Nucleic Acids Res 2004;32(4):1480-91

PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:166019 - Genetic control of radiation sensitivity in Schizosaccharomyces pombe.

Nasim A et al. Genetics 1975 Apr;79(4):573-82

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399