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protein coding gene - aps1 (SPAC13G6.14) - diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1

Gene summary

Standard name
aps1
Systematic ID
SPAC13G6.14
Product
diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC24B11.03, stw1
UniProt ID
Q09790
ORFeome ID
07/07C02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 200038..201517 forward strand

Annotation

GO biological process

GO:1901911 - adenosine 5'-(hexahydrogen pentaphosphate) catabolic process

References:

GO:1901909 - diadenosine hexaphosphate catabolic process

References:

GO:1901907 - diadenosine pentaphosphate catabolic process

References:

GO:0015959 - diadenosine polyphosphate metabolic process

References:

GO:0071543 - diphosphoinositol polyphosphate metabolic process

References:

GO:0071545 - inositol phosphate catabolic process

References:

GO:0030643 - intracellular phosphate ion homeostasis

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0034431 - bis(5'-adenosyl)-hexaphosphatase activity

References:

GO:0034432 - bis(5'-adenosyl)-pentaphosphatase activity

References:

GO:0008796 - bis(5'-nucleosyl)-tetraphosphatase activity

References:

GO:0008486 - diphosphoinositol-polyphosphate diphosphatase activity

References:

GO:0000298 - endopolyphosphatase activity

References:

GO:0052745 - inositol phosphate phosphatase activity

References:

GO:0052846 - inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity

References:

GO:0052847 - inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity

References:

GO:0052843 - inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity

References:

GO:0052845 - inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity

References:

GO:0047710 - bis(5'-adenosyl)-triphosphatase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0005369 - abolished cell population growth at low temperature

References:

Genotypes:

FYPO:0001575 - abolished vegetative cell population growth

References:

Genotypes:

FYPO:0001045 - decreased acid phosphatase activity

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002243 - increased acid phosphatase activity

References:

Genotypes:

FYPO:0001890 - increased RNA level

References:

Genotypes:

FYPO:0002059 - inviable cell population

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003267 - normal acid phosphatase activity

References:

Genotypes:

FYPO:0000047 - normal cell population growth

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0005485 - abolished inositol phosphate phosphatase activity

References:

Genotypes:

FYPO:0008276 - abolished polyphosphatase activity

References:

Genotypes:

FYPO:0003724 - decreased bis(5'-adenosyl)-hexaphosphatase activity

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002243 - increased acid phosphatase activity

References:

Genotypes:

FYPO:0003725 - increased bis(5'-adenosyl)-hexaphosphatase activity

References:

Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0000637 - increased cell population growth rate on glucose carbon source

References:

Genotypes:

FYPO:0008277 - increased cellular 1,5-IP8 level

References:

Genotypes:

FYPO:0008279 - increased cellular 1-IP7 level

References:

Genotypes:

FYPO:0008278 - increased cellular 5-IP7 level

References:

Genotypes:

FYPO:0003726 - increased cellular inositol pentakisphosphate level

References:

Genotypes:

FYPO:0007816 - increased cellular polyphosphate level

References:

Genotypes:

FYPO:0004415 - increased phosphatase activity

References:

Genotypes:

FYPO:0004834 - increased phosphatase activity during cellular response to adenine starvation

References:

Genotypes:

FYPO:0004414 - increased phosphatase activity during cellular response to phosphate starvation

References:

Genotypes:

FYPO:0001890 - increased RNA level

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0006614 - increased termination of RNA polymerase II transcription

References:

Genotypes:

FYPO:0001494 - inviable elongated multiseptate vegetative cell

References:

Genotypes:

FYPO:0000047 - normal cell population growth

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0008275 - normal polyphosphatase activity

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0007474 - variable cell size at division

References:

Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0002377 - viable swollen vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00293NUDIXNUDIX_hydrolase_domPfam
cd04666NUDIX_DIPP2_like_Nudt4DDP-like_NUDIXCDD
PS00893NUDIX_BOXNUDIX_hydrolase_CSPROSITE patterns
PS51462NUDIXNUDIX_hydrolase_domPROSITE profiles
G3DSA:3.90.79.10CATH-Gene3D
SSF55811NUDIX_hydrolase-like_dom_sfSUPERFAMILY
PTHR12629PANTHER

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:10526233 - Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses.
Yamashita YM et al. Genes Cells 1999 Aug;4(8):445-63
PMID:12387729 - Disruption and overexpression of the Schizosaccharomyces pombe aps1 gene, and effects on growth rate, morphology and intracellular diadenosine 5',5"'-P1,P5-pentaphosphate and diphosphoinositol polyphosphate concentrations.
Ingram SW et al. Biochem J 2003 Feb 01;369(Pt 3):519-28
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:31276588 - Inositol pyrophosphates impact phosphate homeostasis via modulation of RNA 3' processing and transcription termination.
Sanchez AM et al. Nucleic Acids Res 2019 Sep 19;47(16):8452-8469
PMID:37772819 - Activities, substrate specificity, and genetic interactions of fission yeast Siw14, a cysteinyl-phosphatase-type inositol pyrophosphatase.
Sanchez AM et al. mBio 2023 Sep 29;14(5):e0205623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.
Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:25547512 - Dissection of the PHO pathway in Schizosaccharomyces pombe using epistasis and the alternate repressor adenine.
Estill M et al. Curr Genet 2015 May;61(2):175-83
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:35012333 - Cleavage-Polyadenylation Factor Cft1 and SPX Domain Proteins Are Agents of Inositol Pyrophosphate Toxicosis in Fission Yeast.
Schwer B et al. mBio 2022 Feb 22;13(1):e0347621
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:10090752 - Schizosaccharomyces pombe Aps1, a diadenosine 5',5' "-P1, P6-hexaphosphate hydrolase that is a member of the nudix (MutT) family of hydrolases: cloning of the gene and characterization of the purified enzyme.
Ingram SW et al. Biochemistry 1999 Mar 23;38(12):3649-55
PMID:38899862 - Suppression of inositol pyrophosphate toxicosis and hyper-repression of the fission yeast PHO regulon by loss-of-function mutations in chromatin remodelers Snf22 and Sol1.
Schwer B et al. mBio 2024 Jun 20;:e0125224
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:38940614 - Activities and genetic interactions of fission yeast Aps1, a Nudix-type inositol pyrophosphatase and inorganic polyphosphatase.
Ghosh S et al. mBio 2024 Jun 28;:e0108424
PMID:32546512 - Inactivation of fission yeast Erh1 de-represses pho1 expression: evidence that Erh1 is a negative regulator of prt lncRNA termination.
Schwer B et al. RNA 2020 Oct;26(10):1334-1344
PMID:25254656 - The Vip1 inositol polyphosphate kinase family regulates polarized growth and modulates the microtubule cytoskeleton in fungi.
Pöhlmann J et al. PLoS Genet 2014 Sep;10(9):e1004586
PMID:35286199 - Mitotic spindle formation in the absence of Polo kinase.
Kim J et al. Proc Natl Acad Sci U S A 2022 Mar 22;119(12):e2114429119
PMID:27255861 - Genetic evidence for involvement of membrane trafficking in the action of 5-fluorouracil.
Hu L et al. Fungal Genet Biol 2016 Aug;93:17-24
PMID:32282918 - Genetic interactions and transcriptomics implicate fission yeast CTD prolyl isomerase Pin1 as an agent of RNA 3' processing and transcription termination that functions via its effects on CTD phosphatase Ssu72.
Sanchez AM et al. Nucleic Acids Res 2020 May 21;48(9):4811-4826
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:21169418 - Systematic screen of Schizosaccharomyces pombe deletion collection uncovers parallel evolution of the phosphate signal transduction pathway in yeasts.
Henry TC et al. Eukaryot Cell 2011 Feb;10(2):198-206
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:19462967 - Kinetic, dynamic, ligand binding properties, and structural models of a dual-substrate specific nudix hydrolase from Schizosaccharomyces pombe.
Garza JA et al. Biochemistry 2009 Jul 07;48(26):6224-39
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33419777 - Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
Scotchman E et al. J Cell Sci 2021 Feb 11;134(3)
PMID:34389684 - Genetic screen for suppression of transcriptional interference identifies a gain-of-function mutation in Pol2 termination factor Seb1.
Schwer B et al. Proc Natl Acad Sci U S A 2021 Aug 17;118(33)
PMID:36882296 - Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination.
Sanchez AM et al. RNA 2023 Jun;29(6):808-825
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36