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protein coding gene - arb1 (SPAC140.03) - argonaute inhibitor protein 1

Gene summary

Standard name
arb1
Systematic ID
SPAC140.03
Product
argonaute inhibitor protein 1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
csp12
UniProt ID
Q9P7B8
ORFeome ID
21/21D03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1899966..1901605 forward strand

Annotation

GO biological process

GO:0140727 - siRNA-mediated pericentric heterochromatin formation

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GO cellular component

GO:0033167 - ARC complex

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GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0005721 - pericentric heterochromatin

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GO molecular function

GO:0060698 - endoribonuclease inhibitor activity

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GO:0008428 - ribonuclease inhibitor activity

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Multi-locus phenotype

FYPO:0006992 - normal chromatin silencing at centromere otr1R

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Genotypes:

FYPO:0000964 - normal growth on thiabendazole

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Genotypes:

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000427 - abnormal G1 to G0 transition

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Genotypes:

FYPO:0000031 - abnormal mating

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Genotypes:

FYPO:0002835 - centromeric outer repeat transcript-derived siRNA absent

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

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Genotypes:

FYPO:0006993 - decreased chromatin silencing at centromere otr1R

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0007009 - decreased heterochromatin assembly by small RNA

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Genotypes:

FYPO:0000888 - decreased histone H3-K9 dimethylation at centromere outer repeat during vegetative growth

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Genotypes:

FYPO:0002386 - decreased protein localization to pericentric heterochromatin at centromere outer repeat region

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Genotypes:

FYPO:0004205 - decreased siRNA level

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0001245 - sensitive to cobalt

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF09692Arb1Arb1PFAM

Orthologs

References / Literature

PMID:17310250 - Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.
Buker SM et al. Nat Struct Mol Biol 2007 Mar;14(3):200-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:22474355 - Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.
Hayashi A et al. Proc Natl Acad Sci U S A 2012 Apr 17;109(16):6159-64
PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.
Bayne EH et al. Genome Biol 2014;15(10):481
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29866182 - RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.
Okazaki K et al. Epigenetics Chromatin 2018 Jun 04;11(1):26
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20178743 - Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.
Halic M et al. Cell 2010 Feb 19;140(4):504-16
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:25730778 - Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex.
Holoch D et al. Nat Struct Mol Biol 2015 Apr;22(4):328-35
PMID:21289066 - Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.
Reddy BD et al. Genes Dev 2011 Feb 01;25(3):214-9
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:24095277 - Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.
Marasovic M et al. Mol Cell 2013 Oct 24;52(2):173-83
PMID:32499408 - The molecular chaperone Hsp90 regulates heterochromatin assembly through stabilizing multiple complexes in fission yeast.
Sun L et al. J Cell Sci 2020 Jul 07;133(13)
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.
Okita AK et al. Commun Biol 2019;2:17
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20062003 - Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast.
Simmer F et al. EMBO Rep 2010 Feb;11(2):112-8
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:27538348 - Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.
Yu Y et al. Sci Rep 2016 Aug 19;6:31752
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:22989756 - RNA interference regulates the cell cycle checkpoint through the RNA export factor, Ptr1, in fission yeast.
Iida T et al. Biochem Biophys Res Commun 2012 Oct 12;427(1):143-7
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.
Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561