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protein coding gene - iec1 (SPAC144.02) - Ino80 complex subunit Iec1

Gene summary

Standard name
iec1
Systematic ID
SPAC144.02
Product
Ino80 complex subunit Iec1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTM0
ORFeome ID
09/09B07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4653049..4654387 forward strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

References:

GO:0140861 - DNA repair-dependent chromatin remodeling

References:

GO:0045815 - transcription initiation-coupled chromatin remodeling

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0031011 - Ino80 complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0000977 - RNA polymerase II transcription regulatory region sequence-specific DNA binding

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000029 - abnormal chromosome segregation

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0006992 - normal chromatin silencing at centromere otr1R

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0003559 - sensitive to doxorubicin

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Protein features

PBO:0111745 - zf-C2H2 type

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000029 - abnormal chromosome segregation

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0002399 - abnormal microtubule cytoskeleton

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0006201 - abnormal regulation of adenine biosynthetic process

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0006203 - decreased cellular dATP level

References:

Genotypes:

FYPO:0003740 - decreased CENP-A containing chromatin assembly

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0006206 - decreased protein localization to chromatin during cellular response to phosphate starvation

References:

Genotypes:

FYPO:0004416 - decreased RNA level during cellular response to phosphate starvation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0002633 - increased cellular dTTP level

References:

Genotypes:

FYPO:0006299 - increased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0000238 - inviable cell upon G0 to G1 transition

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

Genotypes:

FYPO:0006204 - normal cellular dCTP level

References:

Genotypes:

FYPO:0006205 - normal cellular dGTP level

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002619 - normal growth on sodium butyrate

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

FYPO:0002620 - normal growth on trichostatin A

References:

Genotypes:

FYPO:0002389 - normal protein localization to heterochromatin at centromere outer repeat

References:

Genotypes:

FYPO:0006202 - normal RNA level during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0000064 - resistance to 2-deoxyglucose

References:

Genotypes:

FYPO:0001986 - resistance to 5-fluorouracil

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009039 - resistance to potassium chloride

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0000094 - sensitive to benomyl

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0003559 - sensitive to doxorubicin

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0006200 - sensitive to phosphate starvation

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0004983 - sensitive to vorinostat

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0002400 - single microtubule bundle during mitotic interphase

References:

Genotypes:

FYPO:0002215 - viable curved elongated vegetative cell

References:

Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00096zf-C2H2Zf-C2H2_typePfam
PF23561zf-C2H2_15Znf-C2H2_ZIC1-5/GLI1-3-likePfam
PS00028ZINC_FINGER_C2H2_1Zf-C2H2_typePROSITE patterns
PS50157ZINC_FINGER_C2H2_2Zf-C2H2_typePROSITE profiles
SM00355ZnF_C2H2Zf-C2H2_typeSMART
G3DSA:3.30.160.60:FF:000031CATH-FunFam
G3DSA:3.30.160.60:FF:000201CATH-FunFam
G3DSA:3.30.160.60CATH-Gene3D
SSF57667Znf_C2H2_sfSUPERFAMILY
PTHR45718GLI-likePANTHER
CoilCOILS

Orthologs

References / Literature

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:25701403 - Two fission yeast high mobility group box proteins in the maintenance of genomic integrity following doxorubicin insult.
Tang MY et al. Gene 2015 May 10;562(1):70-5
PMID:23365689 - Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.
Tay Z et al. PLoS One 2013;8(1):e55041
PMID:19040720 - Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.
Shevchenko A et al. Genome Biol 2008;9(11):R167
PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.
Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26791325 - Predicting chemotherapeutic drug combinations through gene network profiling.
Nguyen TT et al. Sci Rep 2016 Jan 21;6:18658
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:28904333 - The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
Choi ES et al. Nat Commun 2017 Sep 13;8(1):529
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27481777 - Identification of nuclear genes affecting 2-Deoxyglucose resistance in Schizosaccharomyces pombe.
Vishwanatha A et al. FEMS Yeast Res 2016 Sep;16(6)
PMID:30116786 - Genetic regulation of mitotic competence in G 0 quiescent cells.
Sajiki K et al. Sci Adv 2018 Aug;4(8):eaat5685
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:29214404 - The 19S proteasome regulates subtelomere silencing and facultative heterochromatin formation in fission yeast.
Seo HD et al. Curr Genet 2018 Jun;64(3):741-752
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000002 - Comments
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.
Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561
PMID:39747188 - PhpC NF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.
Srivastav MK et al. Nat Commun 2025 Jan 02;16(1):268
PMID:19933844 - Fission yeast Iec1-ino80-mediated nucleosome eviction regulates nucleotide and phosphate metabolism.
Hogan CJ et al. Mol Cell Biol 2010 Feb;30(3):657-74
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83