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protein coding gene - ade2 (SPAC144.03) - adenylosuccinate synthetase Ade2

Gene summary

Standard name
ade2
Systematic ID
SPAC144.03
Product
adenylosuccinate synthetase Ade2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
min10, min3
UniProt ID
Q02787
ORFeome ID
35/35F09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4654631..4656456 forward strand

Annotation

Complementation

PBO:0007914 - functionally complements E. coli purA

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Disease association

MONDO:0014877 - myopathy, distal, 5

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GO biological process

GO:0044208 - 'de novo' AMP biosynthetic process

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GO:0046086 - adenosine biosynthetic process

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GO:0071276 - cellular response to cadmium ion

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GO:0046040 - IMP metabolic process

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GO:0006164 - purine nucleotide biosynthetic process

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0004019 - adenylosuccinate synthase activity

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GO:0016208 - AMP binding

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GO:0019002 - GMP binding

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GO:0005525 - GTP binding

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GO:0000287 - magnesium ion binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0002829 - decreased cellular phytochelatin level

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0000747 - growth auxotrophic for adenine

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0006933 - resistance to butylated hydroxytoluene

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002705 - sensitive to methionine

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00709Adenylsucc_syntAdenylosuccinate_synthetasePFAM
cd03108AdSSAdenylosuccinate_synthetaseCDD
PS00513ADENYLOSUCCIN_SYN_2Adenylosuccin_syn_Lys_ASPROSITE_PATTERNS
PS01266ADENYLOSUCCIN_SYN_1Adenylosuccin_syn_GTP-bdPROSITE_PATTERNS
SM00788adenylsucc_syntAdenylosuccinate_synthetaseSMART
G3DSA:1.10.300.10:FF:000002FUNFAM
G3DSA:3.90.170.10:FF:000001FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.90.170.10Adenylosuccinate Synthetase, subunit A, domain 3Adenylosuccinate_synth_dom3GENE3D
G3DSA:1.10.300.10Adenylosuccinate Synthetase, subunit A, domain 2Adenylosuccinate_synth_dom2GENE3D
G3DSA:3.40.440.10Adenylosuccinate Synthetase, subunit A, domain 1Adenylosuccinate_synth_dom1GENE3D
PTHR11846ADENYLOSUCCINATE SYNTHETASEAdenylosuccinate_synthetasePANTHER
MF_00011Adenylosucc_synthAdenylosuccinate_synthetaseHAMAP
NF002223PRK01117.1NCBIFAM
TIGR00184purAAdenylosuccinate_synthetaseNCBIFAM

Orthologs

References / Literature

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:4708672 - Regulation of the biosynthesis of purine nucleotides in Schizosaccharomyces pombe. 3. Kinetic studies of adenylosuccinate synthetase.
Nagy M et al. Biochim Biophys Acta 1973 May 05;309(1):1-10
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:1394510 - Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate.
Schweingruber ME et al. Curr Genet 1992 Oct;22(4):289-92
PMID:12136010 - Role of the Tsc1-Tsc2 complex in signaling and transport across the cell membrane in the fission yeast Schizosaccharomyces pombe.
Matsumoto S et al. Genetics 2002 Jul;161(3):1053-63
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
GO_REF:0000104 - Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features.
PMID:499806 - Allelism of methionine-sensitive mutants of Schizosaccharomyces pombe to loci involved in adenine biosynthesis.
Strauss A Genet Res 1979 Jun;33(3):261-8
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:8346915 - Two purine biosynthetic enzymes that are required for cadmium tolerance in Schizosaccharomyces pombe utilize cysteine sulfinate in vitro.
Juang RH et al. Arch Biochem Biophys 1993 Aug 01;304(2):392-401
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:11350031 - Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.
Habara Y et al. RNA 2001 May;7(5):671-81
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23145069 - Identification and functional analysis of the erh1(+) gene encoding enhancer of rudimentary homolog from the fission yeast Schizosaccharomyces pombe.
Krzyzanowski MK et al. PLoS One 2012;7(11):e49059
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
GO_REF:0000002 - Comments
PMID:1448066 - Purine biosynthetic genes are required for cadmium tolerance in Schizosaccharomyces pombe.
Speiser DM et al. Mol Cell Biol 1992 Dec;12(12):5301-10
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12