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protein coding gene - srw1 (SPAC144.13c) - substrate-specific mitotic G1 APC coactivator Srw1/Ste9

Gene summary

Standard name
srw1
Systematic ID
SPAC144.13c
Product
substrate-specific mitotic G1 APC coactivator Srw1/Ste9
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ste9
UniProt ID
O13286
ORFeome ID
33/33C11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4677611..4680193 reverse strand

Annotation

Comment

PBO:0001192 - promoter contains MCB

References:

Disease association

MONDO:0859325 - developmental and epileptic encephalopathy 109

References:

GO biological process

GO:0031145 - anaphase-promoting complex-dependent catabolic process

References:

GO:0031568 - mitotic G1 cell size control checkpoint signaling

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GO:2000134 - negative regulation of G1/S transition of mitotic cell cycle

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GO:1905786 - positive regulation of anaphase-promoting complex-dependent catabolic process

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GO cellular component

GO:0005634 - nucleus

References:

GO molecular function

GO:0010997 - anaphase-promoting complex binding

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GO:1990757 - ubiquitin ligase activator activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0000400 - abnormal cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0001054 - cut, elongated cell

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Genotypes:

FYPO:0000711 - decreased cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0005382 - delayed exit from meiosis

References:

Genotypes:

FYPO:0004108 - increased protein level during mitotic G1 phase

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Genotypes:

FYPO:0004922 - inviable elongated mononucleate aseptate cell with cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0001886 - meiotic cell cycle entry and sporulation in haploid

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Genotypes:

FYPO:0003012 - mitosis with unreplicated DNA

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Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

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Genotypes:

FYPO:0000398 - premature mitotic G1/S phase transition

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Genotypes:

FYPO:0000280 - sterile

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001001 - abnormal cell cycle arrest at mitotic G2/M phase transition during nitrogen starvation

References:

Genotypes:

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

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Genotypes:

FYPO:0001425 - abnormal negative regulation of mitotic DNA replication initiation resulting in complete rereplication

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Genotypes:

FYPO:0000302 - abnormal response to pheromone

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Genotypes:

FYPO:0003345 - abolished cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0004233 - decreased and delayed cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

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Genotypes:

FYPO:0000822 - decreased concentration of P-factor in growth medium

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0002798 - decreased protein degradation during nitrogen starvation

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Genotypes:

FYPO:0000846 - decreased protein degradation during vegetative growth

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Genotypes:

FYPO:0000835 - decreased protein level

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0001152 - decreased RNA level during nitrogen starvation

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Genotypes:

FYPO:0004142 - decreased RNA polymerase II proximal promoter sequence-specific DNA binding

References:

Genotypes:

FYPO:0001128 - decreased septation index

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0003770 - decreased transcriptional response to pheromone

References:

Genotypes:

FYPO:0005382 - delayed exit from meiosis

References:

Genotypes:

FYPO:0005412 - delayed onset of meiosis II

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Genotypes:

FYPO:0000836 - increased protein level

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Genotypes:

FYPO:0005414 - increased protein level during meiosis II

References:

Genotypes:

FYPO:0005416 - increased protein level during meiotic anaphase II

References:

Genotypes:

FYPO:0005401 - increased protein level during mitotic G1 cell cycle arrest in response to nitrogen starvation

References:

Genotypes:

FYPO:0004108 - increased protein level during mitotic G1 phase

References:

Genotypes:

FYPO:0000252 - increased spontaneous diploidization

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

References:

Genotypes:

FYPO:0001000 - normal cell cycle arrest in mitotic G1 phase during nitrogen starvation

References:

Genotypes:

FYPO:0002048 - normal cell morphology during nitrogen starvation

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000760 - normal mating

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Genotypes:

FYPO:0005410 - normal protein degradation during mitosis

References:

Genotypes:

FYPO:0005409 - normal protein level during cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0004110 - normal protein level during exit from mitosis

References:

Genotypes:

FYPO:0004107 - normal protein level during mitotic G2 phase

References:

Genotypes:

FYPO:0001903 - normal septation index

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF24807WD40_CDC20-FzWD40_CDC20-FzPFAM
cd00200WD40CDD
PS00678WD_REPEATS_1WD40_repeat_CSPROSITE_PATTERNS
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
SM00320WD40_4WD40_rptSMART
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR19918CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATEDCdc20/FizzyPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:10921876 - APC(ste9/srw1) promotes degradation of mitotic cyclins in G(1) and is inhibited by cdc2 phosphorylation.
Blanco MA et al. EMBO J 2000 Aug 01;19(15):3945-55
PMID:14648198 - DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe.
Cunliffe L et al. Mol Genet Genomics 2004 Feb;271(1):60-71
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:7975894 - Mating pheromone-induced expression of the mat1-Pm gene of Schizosaccharomyces pombe: identification of signalling components and characterization of upstream controlling elements.
Aono T et al. Yeast 1994 Jun;10(6):757-70
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:14985109 - Cdk inhibitor ste9p/srw1p is involved in response to protein synthesis inhibition in fission yeast.
Sakai T Biochem Biophys Res Commun 2004 Mar 19;315(4):984-90
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:10921878 - Fission yeast Fizzy-related protein srw1p is a G(1)-specific promoter of mitotic cyclin B degradation.
Yamaguchi S et al. EMBO J 2000 Aug 01;19(15):3968-77
PMID:21389117 - Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast.
Kimata Y et al. Mol Biol Cell 2011 May;22(9):1486-94
PMID:17046992 - Fission yeast Tor2 promotes cell growth and represses cell differentiation.
Alvarez B et al. J Cell Sci 2006 Nov 01;119(Pt 21):4475-85
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.
Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99
PMID:17304215 - Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.
Akamatsu Y et al. EMBO J 2007 Mar 07;26(5):1352-62
PMID:21118960 - The fission yeast rDNA-binding protein Reb1 regulates G1 phase under nutritional stress.
Rodríguez-Sánchez L et al. J Cell Sci 2011 Jan 01;124(Pt 1):25-34
PMID:23195958 - Anaphase-promoting complex/cyclosome-mediated proteolysis of Ams2 in the G1 phase ensures the coupling of histone gene expression to DNA replication in fission yeast.
Trickey M et al. J Biol Chem 2013 Jan 11;288(2):928-37
PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.
Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:32361273 - Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.
Stonyte V et al. iScience 2020 May 22;23(5):101063
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:9571240 - Fission yeast Ste9, a homolog of Hct1/Cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase.
Kitamura K et al. Mol Biol Cell 1998 May;9(5):1065-80
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:26527280 - Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.
Kamenz J et al. Mol Cell 2015 Nov 05;60(3):446-59
PMID:3185514 - The role of sterility genes (ste and aff) in the initiation of sexual development in Schizosaccharomyces pombe.
Sipiczki M Mol Gen Genet 1988 Aug;213(2-3):529-34
PMID:1934121 - Pheromone production and response in sterile mutants of fission yeast.
Leupold U et al. Curr Genet 1991 Jul;20(1-2):79-85
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:30154212 - Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription.
Rubio A et al. J Cell Sci 2018 Sep 20;131(18)
PMID:9651503 - Identification of a novel casein kinase-1 homolog in fission yeast Schizosaccharomyces pombe.
Kitamura K et al. Gene 1998 Jul 03;214(1-2):131-7
GO_REF:0000002 - Comments
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:9398669 - A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes.
Yamaguchi S et al. Mol Biol Cell 1997 Dec;8(12):2475-86
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6