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protein coding gene - klp8 (SPAC144.14) - kinesin-like protein Klp8

Gene summary

Standard name
klp8
Systematic ID
SPAC144.14
Product
kinesin-like protein Klp8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTL2
ORFeome ID
45/45C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4681265..4683244 forward strand

Annotation

Disease association

MONDO:0011408 - hereditary spastic paraplegia 10

References:

MONDO:0013656 - intellectual disability, autosomal dominant 9

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MONDO:0014552 - lethal fetal cerebrorenogenitourinary agenesis/hypoplasia syndrome

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MONDO:0054761 - microcephaly 20, primary, autosomal recessive

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MONDO:0013634 - neuropathy, hereditary sensory, type 2C

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MONDO:0012651 - spastic ataxia 2

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MONDO:0700307 - spastic paraplegia 30A, autosomal dominant

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MONDO:0971149 - spastic paraplegia 30B, autosomal recessive

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GO biological process

GO:0007018 - microtubule-based movement

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GO cellular component

GO:0032153 - cell division site

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GO:0051286 - cell tip

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GO:0005737 - cytoplasm

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GO:0005871 - kinesin complex

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GO:0071341 - medial cortical node

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GO:0005874 - microtubule

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GO:0044732 - mitotic spindle pole body

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GO:0005634 - nucleus

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GO molecular function

GO:0005524 - ATP binding

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GO:0016887 - ATP hydrolysis activity

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GO:0008017 - microtubule binding

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GO:0003777 - microtubule motor activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00696 - phosphorylated residue

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Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004622 - abolished mitotic spindle disassembly

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0007182 - decreased cytoplasmic microtubule depolymerization at plus end at cell tip

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Genotypes:

FYPO:0003190 - decreased rate of cytoplasmic microtubule depolymerization during vegetative growth

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000636 - increased cell population growth rate

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Genotypes:

FYPO:0005706 - increased duration of mitotic anaphase B

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Genotypes:

FYPO:0005880 - long interphase microtubules curved around cell end

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Genotypes:

FYPO:0000733 - long mitotic spindle

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0003717 - normal actin cytoskeleton morphology during vegetative growth

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Genotypes:

FYPO:0004097 - normal actomyosin contractile ring contraction

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Genotypes:

FYPO:0004652 - normal actomyosin contractile ring morphology

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0003702 - normal microtubule cytoskeleton morphology during vegetative growth

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Genotypes:

FYPO:0006259 - normal mitotic spindle length during metaphase

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Genotypes:

FYPO:0004429 - normal rate of mitotic spindle elongation

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0002215 - viable curved elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF00225KinesinKinesin_motor_domPfam
cd00106KIScCDD
PS50067KINESIN_MOTOR_2Kinesin_motor_domPROSITE profiles
SM00129KIScKinesin_motor_domSMART
PR00380KINESINHEAVYKinesin_motor_domPRINTS
G3DSA:3.40.850.10:FF:000200CATH-FunFam
G3DSA:3.40.850.10Kinesin_motor_dom_sfCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR47969Kinesin-like_famPANTHER
CoilCOILS

Orthologs

References / Literature

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:24508166 - Dueling kinases regulate cell size at division through the SAD kinase Cdr2.
Deng L et al. Curr Biol 2014 Feb 17;24(4):428-33
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PB_REF:0000003 - Disease Association Curation
PMID:17614284 - The chromatin-remodeling factor FACT contributes to centromeric heterochromatin independently of RNAi.
Lejeune E et al. Curr Biol 2007 Jul 17;17(14):1219-24
PMID:28539404 - Analysis of interphase node proteins in fission yeast by quantitative and superresolution fluorescence microscopy.
Akamatsu M et al. Mol Biol Cell 2017 Nov 07;28(23):3203-3214
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:31276301 - A unique kinesin-like protein, Klp8, is involved in mitosis and cell morphology through microtubule stabilization.
Kashiwazaki J et al. Cytoskeleton (Hoboken) 2019 May;76(5):355-367
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:32435206 - Posttranslational Arginylation Enzyme Arginyltransferase1 Shows Genetic Interactions With Specific Cellular Pathways in vivo .
Wiley DJ et al. Front Physiol 2020;11:427
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000002 - Comments
PMID:19474789 - A spatial gradient coordinates cell size and mitotic entry in fission yeast.
Moseley JB et al. Nature 2009 Jun 11;459(7248):857-60
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:24239120 - Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation.
Syrovatkina V et al. Curr Biol 2013 Dec 02;23(23):2423-9
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:32723864 - Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.
Zheng F et al. J Biol Chem 2020 Sep 18;295(38):13287-13298
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8