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protein coding gene - thi1 (SPAC1486.10) - DNA-binding transcription factor Thi1

Gene summary

Standard name
thi1
Systematic ID
SPAC1486.10
Product
DNA-binding transcription factor Thi1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ntf1, SPAC6G10.01
UniProt ID
P36598
ORFeome ID
28/28D02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3211873..3214509 forward strand

Annotation

GO biological process

GO:0090180 - positive regulation of thiamine biosynthetic process

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO:0006357 - regulation of transcription by RNA polymerase II

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GO cellular component

GO:0005634 - nucleus

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GO molecular function

GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific

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GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

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GO:0008270 - zinc ion binding

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Modification

MOD:00046 - O-phospho-L-serine

References:

Protein features

PBO:0111744 - zf-fungal Zn(2)-Cys(6) binuclear cluster domain

PBO:0111743 - zinc finger protein

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Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000583 - abolished sporulation

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Genotypes:

FYPO:0003796 - abolished transcription from RNA polymerase II promoter

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

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Genotypes:

FYPO:0001244 - decreased RNA level during cellular response to thiamine starvation

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Genotypes:

FYPO:0000781 - decreased transcription during vegetative growth

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001907 - growth auxotrophic for thiamine

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Genotypes:

FYPO:0000780 - increased transcription during vegetative growth

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0003798 - normal meiosis II

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0003644 - RNA absent from cell during cellular response to thiamine starvation

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000110 - orthologs cannot be distinguished

Protein features

IDNameInterPro nameDB name
PF00172Zn_clusZn2Cys6_DnaBDPFAM
PF04082Fungal_transXnlR_reg_domPFAM
cd12148fungal_TF_MHRCDD
cd00067GAL4Zn2Cys6_DnaBDCDD
PS00463ZN2_CY6_FUNGAL_1Zn2Cys6_DnaBDPROSITE_PATTERNS
PS50048ZN2_CY6_FUNGAL_2Zn2Cys6_DnaBDPROSITE_PROFILES
SM00906Fungal_trans_2XnlR_reg_domSMART
SM00066gal4_2Zn2Cys6_DnaBDSMART
G3DSA:4.10.240.10:FF:000028FUNFAM
SSF57701Zn2/Cys6 DNA-binding domainZn2-C6_fun-type_DNA-bd_sfSUPERFAMILY
G3DSA:4.10.240.10Zn2-C6_fun-type_DNA-bd_sfGENE3D
PTHR47540THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5PANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder

Orthologs

References / Literature

PMID:16874521 - Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe.
McQuire TA et al. Curr Genet 2006 Oct;50(4):269-79
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:12653111 - Thiamin regulates agglutination and zygote formation in Schizosaccharomyces pombe.
Schweingruber ME et al. Curr Genet 1990 Mar;17(3):191-4
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000002 - Comments
PMID:7961415 - Cloning, nucleotide sequence, and regulation of Schizosaccharomyces pombe thi4, a thiamine biosynthetic gene.
Zurlinden A et al. J Bacteriol 1994 Nov;176(21):6631-5
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:8163491 - ntf1+ encodes a 6-cysteine zinc finger-containing transcription factor that regulates the nmt1 promoter in fission yeast.
Tang CS et al. J Biol Chem 1994 Apr 22;269(16):11921-6
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:8675019 - Amiloride toxicity in the fission yeast Schizosaccharomyces pombe is released by thiamine and mutations in the thiamine-repressible gene car1.
Niederberger C et al. Gene 1996 May 24;171(1):119-22
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:8088540 - Thiamine-repressible genes in Schizosaccharomyces pombe are regulated by a Cys6 zinc-finger motif-containing protein.
Fankhauser H et al. Gene 1994 Sep 15;147(1):141-4
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:1644306 - Cloning and regulation of Schizosaccharomyces pombe thi2, a gene involved in thiamine biosynthesis.
Zurlinden A et al. Gene 1992 Aug 01;117(1):141-3