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protein coding gene - agn1 (SPAC14C4.09) - cell wall glucan endo-1,3-alpha-glucosidase Agn1

Gene summary

Standard name
agn1
Systematic ID
SPAC14C4.09
Product
cell wall glucan endo-1,3-alpha-glucosidase Agn1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13716
ORFeome ID
22/22B03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5244037..5245966 forward strand

Annotation

Comment

PBO:0002897 - promoter contains UAS

References:

GO biological process

GO:0030995 - cell septum edging catabolic process

References:

GO:1904541 - mating projection tip cell wall disassembly

References:

GO cellular component

GO:0005576 - extracellular region

References:

GO:1990819 - mating projection actin fusion focus

References:

GO:0043332 - mating projection tip

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GO molecular function

GO:0051118 - glucan endo-1,3-alpha-glucosidase activity

References:

Multi-locus phenotype

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

References:

Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

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Genotypes:

FYPO:0003500 - viable branched, elongated, multiseptate vegetative cell

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

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Qualitative gene expression

PomGeneEx:0000024 - protein level fluctuates

References:

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

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Genotypes:

FYPO:0001018 - abolished NETO

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Genotypes:

FYPO:0004273 - decreased ascospore release from ascus

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0004270 - decreased cell septum edging catabolic process

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009076 - increased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0000239 - increased transcription from MCB promoter

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0003334 - normal protein localization to septin ring

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Genotypes:

FYPO:0000673 - normal septum assembly

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Genotypes:

FYPO:0004267 - normal septum disassembly

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0002634 - resistance to cobalt

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0009040 - resistance to tea tree oil

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Subunit composition

PBO:0001467 - monomeric

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF03659Glyco_hydro_71Glyco_hydro_71Pfam
cd11577GH71Glyco_hydro_71CDD
G3DSA:3.20.20.80:FF:000268CATH-FunFam
G3DSA:3.20.20.80CATH-Gene3D
Signal PeptideDeepTMHMM-Signal-Peptide

Orthologs

References / Literature

PMID:15194814 - Role of the alpha-glucanase Agn1p in fission-yeast cell separation.
Dekker N et al. Mol Biol Cell 2004 Aug;15(8):3903-14
PMID:16317047 - Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression.
Petit CS et al. J Cell Sci 2005 Dec 15;118(Pt 24):5731-42
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25825517 - A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
Dudin O et al. J Cell Biol 2015 Mar 30;208(7):897-911
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:25015293 - Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex.
Lei B et al. J Cell Sci 2014 Sep 15;127(Pt 18):4024-36
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:32182184 - Cdc42 promotes Bgs1 recruitment for septum synthesis and glucanase localization for cell separation during cytokinesis in fission yeast.
Onwubiko UN et al. Small GTPases 2021 Jul;12(4):257-264
PMID:15689498 - Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe.
Alonso-Nuñez ML et al. Mol Biol Cell 2005 Apr;16(4):2003-17
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26890608 - A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
Gaspa L et al. Cell Cycle 2016;15(5):720-9
PMID:23093943 - Cytokinesis-based constraints on polarized cell growth in fission yeast.
Bohnert KA et al. PLoS Genet 2012;8(10):e1003004
PMID:39666777 - Fission yeast essential nuclear pore protein Nup211 regulates the expression of genes involved in cytokinesis.
Kamel D et al. PLoS One 2024;19(12):e0312095
PMID:25411334 - The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.
Wang N et al. Mol Biol Cell 2015 Jan 15;26(2):238-55
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:16215171 - Rho4 GTPase is involved in secretion of glucanases during fission yeast cytokinesis.
Santos B et al. Eukaryot Cell 2005 Oct;4(10):1639-45
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:22905165 - The integrity of the cytokinesis machinery under stress conditions requires the glucan synthase Bgs1p and its regulator Cfh3p.
Sharifmoghadam MR et al. PLoS One 2012;7(8):e42726
PMID:18272786 - Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast.
Ge W et al. Mol Biol Cell 2008 Apr;19(4):1680-92
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:34910579 - Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.
Longo LVG et al. Mol Biol Cell 2022 Feb 01;33(2):ar17
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39660919 - Efn1 and Efn2 are extracellular 5'-nucleotidases induced during the fission yeast response to phosphate starvation.
Innokentev A et al. mBio 2024 Dec 11;:e0299224
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16678171 - Transcription regulation of the alpha-glucanase gene agn1 by cell separation transcription factor Ace2p in fission yeast.
Dekker N et al. FEBS Lett 2006 May 29;580(13):3099-106
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:24514900 - Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1.
Curto MÁ et al. Genetics 2014 Apr;196(4):1059-76
PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.
Jin QW et al. Genetics 2006 Apr;172(4):2101-12
PMID:24972934 - Cross talk between NDR kinase pathways coordinates cytokinesis with cell separation in Schizosaccharomyces pombe.
Gupta S et al. Eukaryot Cell 2014 Aug;13(8):1104-12
PMID:31116668 - Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis.
Morris Z et al. Mol Biol Cell 2019 Jul 15;30(15):1791-1804