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protein coding gene - cbs2 (SPAC1556.08c) - serine/threonine protein kinase AMPK (gamma) subunit Cbs2

Gene summary

Standard name
cbs2
Systematic ID
SPAC1556.08c
Product
serine/threonine protein kinase AMPK (gamma) subunit Cbs2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC1F12.01c
UniProt ID
Q10343
ORFeome ID
19/19D12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3807132..3808702 reverse strand

Annotation

Disease association

MONDO:0002412 - disorder of glycogen metabolism

References:

MONDO:0005267 - heart disorder

References:

MONDO:0010946 - hypertrophic cardiomyopathy 6

References:

MONDO:0009867 - lethal congenital glycogen storage disease of heart

References:

MONDO:0005066 - metabolic disease

References:

MONDO:0008685 - Wolff-Parkinson-White syndrome

References:

GO biological process

GO:0042149 - cellular response to glucose starvation

References:

GO:0031139 - positive regulation of conjugation with cellular fusion

References:

GO:0045722 - positive regulation of gluconeogenesis

References:

GO:0006110 - regulation of glycolysis

References:

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO:0007165 - signal transduction

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0031588 - nucleotide-activated protein kinase complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0043531 - ADP binding

References:

GO:0016208 - AMP binding

References:

GO:0005524 - ATP binding

References:

GO:0005515 - protein binding

References:

GO:0030295 - protein kinase activator activity

References:

Multi-locus phenotype

FYPO:0007434 - attenuated change in cell size at division upon shift to poor nitrogen source

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0004422 - normal protein phosphorylation

References:

Genotypes:

FYPO:0005193 - resistance to torin1

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0007434 - attenuated change in cell size at division upon shift to poor nitrogen source

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0005746 - decreased protein export from nucleus during glucose starvation

References:

Genotypes:

FYPO:0002376 - decreased protein phosphorylation during cellular response to osmotic stress

References:

Genotypes:

FYPO:0005749 - decreased protein phosphorylation during glucose starvation

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0003896 - normal mitochondrial morphology

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0005968 - resistance to sodium chloride

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0005193 - resistance to torin1

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0017738 - heteromeric(6)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00571CBSCBS_domPfam
cd04618CBS_euAMPK_gamma-like_repeat1CDD
cd04641CBS_euAMPK_gamma-like_repeat2CDD
PS51371CBSCBS_domPROSITE profiles
SM00116CBSCBS_domSMART
G3DSA:3.10.580.10:FF:000033CATH-FunFam
G3DSA:3.10.580.10:FF:000049CATH-FunFam
G3DSA:3.10.580.10CBS_dom_sfCATH-Gene3D
SSF54631CBS_dom_sfSUPERFAMILY
PTHR13780AMPK_gamma/SDS23_familiesPANTHER

Orthologs

References / Literature

PMID:22140232 - Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe.
Matsuzawa T et al. Eukaryot Cell 2012 Feb;11(2):159-67
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:34706246 - Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns.
Hümmer S et al. Cell Rep 2021 Oct 26;37(4):109893
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:22375066 - AMPK phosphorylation by Ssp1 is required for proper sexual differentiation in fission yeast.
Valbuena N et al. J Cell Sci 2012 Jun 01;125(Pt 11):2655-64
PMID:28223368 - Phosphatases Generate Signal Specificity Downstream of Ssp1 Kinase in Fission Yeast.
Deng L et al. Mol Cell Biol 2017 May 15;37(10)
PMID:17289942 - Crystal structures of the adenylate sensor from fission yeast AMP-activated protein kinase.
Townley R et al. Science 2007 Mar 23;315(5819):1726-9
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:9660817 - Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast.
Yang P et al. J Biol Chem 1998 Jul 17;273(29):18481-9
PMID:17937917 - Structural insight into AMPK regulation: ADP comes into play.
Jin X et al. Structure 2007 Oct;15(10):1285-95
PMID:31562247 - Glucose starvation induces mitochondrial fragmentation depending on the dynamin GTPase Dnm1/Drp1 in fission yeast.
Zheng F et al. J Biol Chem 2019 Nov 22;294(47):17725-17734
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19371376 - Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor.
Hanyu Y et al. Genes Cells 2009 May;14(5):539-54
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:25639242 - Nitrogen regulates AMPK to control TORC1 signaling.
Davie E et al. Curr Biol 2015 Feb 16;25(4):445-54
PMID:25411338 - Mechanisms of expression and translocation of major fission yeast glucose transporters regulated by CaMKK/phosphatases, nuclear shuttling, and TOR.
Saitoh S et al. Mol Biol Cell 2015 Jan 15;26(2):373-86
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PB_REF:0000003 - Disease Association Curation
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000051 - S. pombe keyword mapping
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:28515144 - Transient activation of fission yeast AMPK is required for cell proliferation during osmotic stress.
Schutt KL et al. Mol Biol Cell 2017 Jul 01;28(13):1804-1814