protein coding gene - rad54 (SPAC15A10.03c) - DNA-dependent ATPase Rad54/Rhp54

Gene summary

Standard name

rad54

Systematic ID

SPAC15A10.03c

Product

DNA-dependent ATPase Rad54/Rhp54

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

rhp54

UniProt ID

P41410

ORFeome ID

39/39G01

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 3680360..3683265 reverse strand

Annotation

Protein features

Orthologs

• RAD54L (HGNC:9826) Homo sapiens
• rad54 (SJAG_02460) Schizosaccharomyces japonicus
• RAD54 (YGL163C) Saccharomyces cerevisiae

References / Literature

PMID:17660542 - Mms22 preserves genomic integrity during DNA replication in Schizosaccharomyces pombe.

Dovey CL et al. Genetics 2007 Sep;177(1):47-61

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:10373582 - The role of Schizosaccharomyces pombe Rad32, the Mre11 homologue, and other DNA damage response proteins in non-homologous end joining and telomere length maintenance.

Wilson S et al. Nucleic Acids Res 1999 Jul 01;27(13):2655-61

PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.

Takeda K et al. PLoS One 2011;6(7):e22021

PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33

PMID:9870697 - Differential effects of caffeine on DNA damage and replication cell cycle checkpoints in the fission yeast Schizosaccharomyces pombe.

Osman F et al. Mol Gen Genet 1998 Nov;260(4):319-34

PMID:17178839 - Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase.

Kearsey SE et al. Mol Cell Biol 2007 Mar;27(5):1558-67

PMID:15802523 - A postsynaptic role for Rhp55/57 that is responsible for cell death in Deltarqh1 mutants following replication arrest in Schizosaccharomyces pombe.

Hope JC et al. Genetics 2005 Jun;170(2):519-31

PMID:18231579 - The CDK-activating kinase (CAK) Csk1 is required for normal levels of homologous recombination and resistance to DNA damage in fission yeast.

Gerber HB et al. PLoS One 2008 Jan 30;3(1):e1492

PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.

Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235

PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.

Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)

PMID:11226171 - Novel functional requirements for non-homologous DNA end joining in Schizosaccharomyces pombe.

Manolis KG et al. EMBO J 2001 Jan 15;20(1-2):210-21

PMID:24385927 - The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast.

Mbogning J et al. PLoS Genet 2013;9(12):e1004029

PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.

Malecki M et al. Genome Biol 2016 Nov 25;17(1):240

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:14993467 - The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.

Doe CL et al. Nucleic Acids Res 2004;32(4):1480-91

PMID:15367656 - Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.

Noguchi E et al. Mol Cell Biol 2004 Oct;24(19):8342-55

PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.

Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619

PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

Pan X et al. BMC Genomics 2012 Nov 23;13:662

PMID:12724426 - Role for the fission yeast RecQ helicase in DNA repair in G2.

Laursen LV et al. Mol Cell Biol 2003 May;23(10):3692-705

PMID:15643072 - Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.

Williams DR et al. Eukaryot Cell 2005 Jan;4(1):166-77

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:27304859 - Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.

Polakova S et al. PLoS Genet 2016 Jun;12(6):e1006102

PMID:14551247 - Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks.

Catlett MG et al. Mol Biol Cell 2003 Nov;14(11):4707-20

PMID:18426916 - The anaphase-promoting complex/cyclosome controls repair and recombination by ubiquitylating Rhp54 in fission yeast.

Trickey M et al. Mol Cell Biol 2008 Jun;28(12):3905-16

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:23365689 - Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.

Tay Z et al. PLoS One 2013;8(1):e55041

PMID:8834792 - Isolation of the Schizosaccharomyces pombe RAD54 homologue, rhp54+, a gene involved in the repair of radiation damage and replication fidelity.

Muris DF et al. J Cell Sci 1996 Jan;109 ( Pt 1):73-81

PMID:11884628 - Differential expression and requirements for Schizosaccharomyces pombe RAD52 homologs in DNA repair and recombination.

van den Bosch M et al. Nucleic Acids Res 2002 Mar 15;30(6):1316-24

GO_REF:0000002 - Comments

PMID:21084840 - The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe.

Tougan T et al. Cell Cycle 2010 Dec 01;9(23):4688-702

PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.

Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400

PMID:25701403 - Two fission yeast high mobility group box proteins in the maintenance of genomic integrity following doxorubicin insult.

Tang MY et al. Gene 2015 May 10;562(1):70-5

GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model

PMID:10430583 - A new recombinational DNA repair gene from Schizosaccharomyces pombe with homology to Escherichia coli RecA.

Khasanov FK et al. Genetics 1999 Aug;152(4):1557-72

PMID:26791325 - Predicting chemotherapeutic drug combinations through gene network profiling.

Nguyen TT et al. Sci Rep 2016 Jan 21;6:18658

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:11560889 - Multiple interactions among the components of the recombinational DNA repair system in Schizosaccharomyces pombe.

Tsutsui Y et al. Genetics 2001 Sep;159(1):91-105

PMID:17304215 - Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.

Akamatsu Y et al. EMBO J 2007 Mar 07;26(5):1352-62

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:27697832 - Rad51 and Rad54 promote noncrossover recombination between centromere repeats on the same chromatid to prevent isochromosome formation.

Onaka AT et al. Nucleic Acids Res 2016 Dec 15;44(22):10744-10757

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:28922417 - Lingering single-strand breaks trigger Rad51-independent homology-directed repair of collapsed replication forks in the polynucleotide kinase/phosphatase mutant of fission yeast.

Sanchez A et al. PLoS Genet 2017 Sep;13(9):e1007013

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:15972456 - Brc1-mediated DNA repair and damage tolerance.

Sheedy DM et al. Genetics 2005 Oct;171(2):457-68

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.

Materne P et al. Elife 2015 Jun 22;4:e09008

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:28334931 - Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast.

Manjón E et al. Nucleic Acids Res 2017 May 19;45(9):5269-5284

PMID:18769921 - Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair.

Khasanov FK et al. Curr Genet 2008 Oct;54(4):197-211

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:18388861 - Mus81 is essential for sister chromatid recombination at broken replication forks.

Roseaulin L et al. EMBO J 2008 May 07;27(9):1378-87

PMID:12409469 - The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes.

Maftahi M et al. Nucleic Acids Res 2002 Nov 01;30(21):4781-92

PMID:17724078 - Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.

Cullen JK et al. Mol Cell Biol 2007 Nov;27(21):7745-57

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:36951094 - Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching.

Maki T et al. Nucleic Acids Res 2023 May 08;51(8):3869-3887

PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.

Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202

PMID:23738021 - A genome-wide screening of potential target genes to enhance the antifungal activity of micafungin in Schizosaccharomyces pombe.

Zhou X et al. PLoS One 2013;8(5):e65904

PMID:11452021 - Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage.

Wang SW et al. Nucleic Acids Res 2001 Jul 15;29(14):2963-72

PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.

Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:12419233 - Increased recombination intermediates and homologous integration hot spots at DNA replication origins.

Segurado M et al. Mol Cell 2002 Oct;10(4):907-16

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83