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protein coding gene - bud6 (SPAC15A10.16) - actin nucleation-promoting factor Bud6/Aip3

Gene summary

Standard name
bud6
Systematic ID
SPAC15A10.16
Product
actin nucleation-promoting factor Bud6/Aip3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
aip3, fat1, SPAC15E1.01
UniProt ID
O13735
ORFeome ID
30/30F12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3716643..3720963 forward strand

Annotation

Complementation

PBO:0091494 - hal3 N-term functionally complements S. cerevisiae HAL3

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GO biological process

GO:0045010 - actin nucleation

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GO:0030010 - establishment of cell polarity

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GO cellular component

GO:0032153 - cell division site

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GO:0051286 - cell tip

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GO:0005737 - cytoplasm

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GO molecular function

GO:0003779 - actin binding

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GO:0005519 - cytoskeletal regulatory protein binding

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Modification

MOD:00595 - monomannosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0002558 - normal protein localization to medial cortex during vegetative growth

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Genotypes:

FYPO:0002253 - normal septum location

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Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

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Genotypes:

FYPO:0004103 - viable spherical vegetative cell

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0003208 - decreased protein localization to cell tip, with protein distributed in plasma membrane or cortex

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

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Genotypes:

FYPO:0007451 - normal protein localization to cell cortex of cell tip during vegetative growth

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Genotypes:

FYPO:0002442 - normal protein localization to cell division site

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0007996 - thin actin cables

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF23153Aip3p_Bud6_NAip3p_Bud6_NPFAM
PF03915AIP3AIP3-like_CPFAM
SM00806aip3AIP3_CSMART
G3DSA:1.20.58.1540GENE3D
PTHR22741P140CAP/SNIP-RELATEDSRCIN1PANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:20870879 - Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation.
Dodgson J et al. Eukaryot Cell 2010 Nov;9(11):1788-97
GO_REF:0000002 - Comments
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:15837798 - Efficient formation of bipolar microtubule bundles requires microtubule-bound gamma-tubulin complexes.
Janson ME et al. J Cell Biol 2005 Apr 25;169(2):297-308
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:39110593 - VAP-mediated membrane-tethering mechanisms implicate ER-PM contact function in pH homeostasis.
Hoh KL et al. Cell Rep 2024 Aug 05;43(8):114592
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40931936 - Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.
Ren L et al. J Cell Sci 2025 Sep 01;138(17)
PMID:18505873 - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregation.
Gómez EB et al. Genetics 2008 Jun;179(2):757-71
PMID:18060866 - ADP-ribosylation factor arf6p may function as a molecular switch of new end take off in fission yeast.
Fujita A Biochem Biophys Res Commun 2008 Feb 01;366(1):193-8
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:24316795 - Distinct levels in Pom1 gradients limit Cdr2 activity and localization to time and position division.
Bhatia P et al. Cell Cycle 2014;13(4):538-52
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:11516644 - Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast.
Glynn JM et al. Curr Biol 2001 Jun 05;11(11):836-45
PMID:19597328 - Role of the protein kinase Kin1 and nuclear centering in actomyosin ring formation in fission yeast.
Cadou A et al. Cell Cycle 2009 Aug;8(15):2451-62
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:16611237 - Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic gamma-tubulin complexes.
Masuda H et al. Genes Cells 2006 Apr;11(4):319-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:12789340 - Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips.
Snaith HA et al. Nature 2003 Jun 05;423(6940):647-51
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15184402 - Regulation of a formin complex by the microtubule plus end protein tea1p.
Feierbach B et al. J Cell Biol 2004 Jun 07;165(5):697-707
PANTHER:PTHR22741 - Unknown title
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:23962284 - The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities.
Molero C et al. Mol Microbiol 2013 Oct;90(2):367-82
PMID:17895368 - Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth.
Alvarez-Tabarés I et al. J Cell Sci 2007 Oct 15;120(Pt 20):3589-601