PomBase home

protein coding gene - moa1 (SPAC15E1.07c) - meiosis specific kinetochore regulator (Meikin) Moa1

Gene summary

Standard name
moa1
Systematic ID
SPAC15E1.07c
Product
meiosis specific kinetochore regulator (Meikin) Moa1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mug159
UniProt ID
Q9UTI4
ORFeome ID
05/05B07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3728516..3729034 reverse strand

Annotation

Complementation

PBO:0018248 - functionally complemented by S. cerevisiae SPO13

References:

GO biological process

GO:0031619 - homologous chromosome orientation in meiotic metaphase I

References:

GO:0051321 - meiotic cell cycle

References:

GO:1990813 - meiotic centromeric cohesion protection in anaphase I

References:

GO:0045132 - meiotic chromosome segregation

References:

GO:0051455 - spindle attachment to meiosis I kinetochore

References:

GO cellular component

GO:0061638 - CENP-A containing chromatin

References:

GO:0000776 - kinetochore

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0140483 - kinetochore adaptor activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0005642 - abnormal meiotic homologous chromosome biorientation with abnormal kinetochore orientation

References:

Genotypes:

FYPO:0007081 - abolished initial meiotic spindle pole body separation in meiosis I

References:

Genotypes:

FYPO:0006424 - abolished meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0001734 - abolished mitotic spindle pole body separation

References:

Genotypes:

FYPO:0004763 - abolished protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0004212 - decreased protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0004393 - lagging chromosomes during meiosis I

References:

Genotypes:

FYPO:0007083 - meiotic spindle collapse

References:

Genotypes:

FYPO:0006475 - mitotic spindle collapse

References:

Genotypes:

FYPO:0006425 - normal meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0003178 - normal meiotic sister chromatid segregation

References:

Genotypes:

FYPO:0004214 - normal protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

References:

Genotypes:

FYPO:0005633 - sister kinetochore dissociation in meiotic metaphase I, normal chromosome segregation in meiosis I, and sister chromatid non-disjunction in meiosis II

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

References:

Genotypes:

FYPO:0006424 - abolished meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0004763 - abolished protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0006423 - decreased meiotic sister chromatid cohesion at centromere during meiosis I

References:

Genotypes:

FYPO:0004212 - decreased protein localization to kinetochore during meiosis I

References:

Genotypes:

FYPO:0003537 - decreased rate of premeiotic DNA replication

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0007295 - increased protein localization to CENP-A containing chromatin

References:

Genotypes:

FYPO:0008190 - increased protein localization to chromatin at centromere central core during meiotic prophase I

References:

Genotypes:

FYPO:0008192 - increased protein localization to pericentric heterochromatin during meiotic prophase I

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0000488 - normal meiotic recombination

References:

Genotypes:

FYPO:0004314 - normal protein localization to CENP-A containing chromatin

References:

Genotypes:

FYPO:0008193 - normal protein localization to pericentric heterochromatin during meiotic prophase I

References:

Genotypes:

FYPO:0002052 - normal sporulation frequency

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005648 - sister kinetochore dissociation in meiotic metaphase I

References:

Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

References:

Genotypes:

FYPO:0005633 - sister kinetochore dissociation in meiotic metaphase I, normal chromosome segregation in meiosis I, and sister chromatid non-disjunction in meiosis II

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

References:

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

References:

PBO:0011069 - conserved in metazoa

References:

PBO:0011070 - conserved in vertebrates

References:

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMOBIDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:12748297 - Cohesins determine the attachment manner of kinetochores to spindle microtubules at meiosis I in fission yeast.
Yokobayashi S et al. Mol Cell Biol 2003 Jun;23(11):3965-73
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33888556 - Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I.
Ma W et al. Genes Dev 2021 May 01;35(9-10):692-697
PMID:31366733 - Structures of CENP-C cupin domains at regional centromeres reveal unique patterns of dimerization and recruitment functions for the inner pocket.
Chik JK et al. J Biol Chem 2019 Sep 20;294(38):14119-14134
PMID:29196561 - Hierarchical Regulation of Centromeric Cohesion Protection by Meikin and Shugoshin during Meiosis I.
Miyazaki S et al. Cold Spring Harb Symp Quant Biol 2017;82:259-266
PMID:34851403 - Direct evaluation of cohesin-mediated sister kinetochore associations at meiosis I in fission yeast.
Nambu M et al. J Cell Sci 2022 Jan 01;135(1)
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25533956 - Meikin is a conserved regulator of meiosis-I-specific kinetochore function.
Kim J et al. Nature 2015 Jan 22;517(7535):466-71
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:21979813 - Acetylation regulates monopolar attachment at multiple levels during meiosis I in fission yeast.
Kagami A et al. EMBO Rep 2011 Oct 28;12(11):1189-95
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:16325576 - The kinetochore protein Moa1 enables cohesion-mediated monopolar attachment at meiosis I.
Yokobayashi S et al. Cell 2005 Dec 02;123(5):803-17
PMID:31532702 - Kinetochore-mediated outward force promotes spindle pole separation in fission yeast.
Shirasugi Y et al. Mol Biol Cell 2019 Oct 15;30(22):2802-2813
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.
Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567
PMID:11102508 - A conserved interaction between Moe1 and Mal3 is important for proper spindle formation in Schizosaccharomyces pombe.
Chen CR et al. Mol Biol Cell 2000 Dec;11(12):4067-77
PMID:32761854 - Meiosis I Kinase Regulators: Conserved Orchestrators of Reductional Chromosome Segregation.
Galander S et al. Bioessays 2020 Oct;42(10):e2000018
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:38448160 - Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis I.
Liu Y et al. Life Sci Alliance 2024 May;7(5)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:32790622 - Atypical meiosis can be adaptive in outcrossed Schizosaccharomyces pombe due to wtf meiotic drivers.
Bravo Núñez MA et al. Elife 2020 Aug 13;9
PMID:16303567 - A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events.
Martín-Castellanos C et al. Curr Biol 2005 Nov 22;15(22):2056-62
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:23658229 - Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast.
Sugiyama T et al. Nucleic Acids Res 2013 Jul;41(13):6674-86
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:25579976 - Phosphorylation of cohesin Rec11/SA3 by casein kinase 1 promotes homologous recombination by assembling the meiotic chromosome axis.
Sakuno T et al. Dev Cell 2015 Jan 26;32(2):220-30
PMID:19758558 - CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis.
Tanaka K et al. Dev Cell 2009 Sep;17(3):334-43
PMID:19370027 - Kinetochore geometry defined by cohesion within the centromere.
Sakuno T et al. Nature 2009 Apr 16;458(7240):852-8