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protein coding gene - rho2 (SPAC16.01) - Rho family GTPase Rho2

Gene summary

Standard name
rho2
Systematic ID
SPAC16.01
Product
Rho family GTPase Rho2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10133
ORFeome ID
18/18F05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4422691..4424646 forward strand

Annotation

Disease association

MONDO:0032884 - ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies

References:

GO biological process

GO:0030950 - establishment or maintenance of actin cytoskeleton polarity

References:

GO:1902660 - negative regulation of glucose mediated signaling pathway

References:

GO:1903139 - positive regulation of cell integrity MAPK cascade

References:

GO:0032956 - regulation of actin cytoskeleton organization

References:

GO:0070610 - regulation of fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

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GO cellular component

GO:0032153 - cell division site

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GO:0005829 - cytosol

References:

GO:0035841 - new growing cell tip

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GO:0005634 - nucleus

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GO:0035840 - old growing cell tip

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GO:0005886 - plasma membrane

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GO molecular function

GO:0005525 - GTP binding

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GO:0003924 - GTPase activity

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GO:0005515 - protein binding

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GO:0043539 - protein serine/threonine kinase activator activity

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Modification

MOD:00437 - farnesylated residue

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MOD:00441 - geranylgeranylated residue

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MOD:00114 - L-cysteine methyl ester

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0006709 - abolished protein phosphorylation during cellular response to salt stress

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Genotypes:

FYPO:0004700 - bent vegetative cell

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Genotypes:

FYPO:0001235 - decreased extent of cell population growth

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Genotypes:

FYPO:0001382 - decreased protein kinase activity

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

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Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0005092 - inviable after spore germination, multiple cell divisions, swollen, elongated pear-shaped cell

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001884 - resistance to Calcofluor White

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Genotypes:

FYPO:0001882 - resistance to cell wall-degrading enzymes

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0001379 - resistance to tacrolimus

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Genotypes:

FYPO:0001473 - resistance to tacrolimus during salt stress

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Genotypes:

FYPO:0001966 - sensitive to aculeacin A

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Genotypes:

FYPO:0000079 - sensitive to caspofungin

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

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Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111810 - Rho family

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001118 - abnormal vegetative cell morphology

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Genotypes:

FYPO:0008263 - abolished protein kinase activity in response to osmotic stress

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0001382 - decreased protein kinase activity

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Genotypes:

FYPO:0001885 - decreased protein phosphorylation during salt stress

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

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Genotypes:

FYPO:0002297 - dispersed actin cortical patch localization during mitotic interphase

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Genotypes:

FYPO:0005197 - increased protein phosphorylation during glucose starvation

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Genotypes:

FYPO:0001571 - increased protein-protein interaction

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Genotypes:

FYPO:0001406 - increased septum thickness

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

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Genotypes:

FYPO:0004104 - inviable spherical vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0002424 - normal actin cortical patch localization during mitotic interphase

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Genotypes:

FYPO:0002229 - normal cellular ergosterol level

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

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Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0003503 - normal vegetative cell length

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0001884 - resistance to Calcofluor White

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

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Genotypes:

FYPO:0001379 - resistance to tacrolimus

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Genotypes:

FYPO:0001473 - resistance to tacrolimus during salt stress

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0001966 - sensitive to aculeacin A

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0002642 - sensitive to amphotericin B

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Genotypes:

FYPO:0002720 - sensitive to beta-glucanase

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0007926 - sensitive to calcofluor and potassium chloride

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000099 - sensitive to canavanine

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Genotypes:

FYPO:0000079 - sensitive to caspofungin

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Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

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Genotypes:

FYPO:0002640 - sensitive to clotrimazole

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Genotypes:

FYPO:0001245 - sensitive to cobalt

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0002641 - sensitive to micafungin

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000113 - sensitive to staurosporine

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00071RasSmall_GTPasePFAM
cd04129Rho2CDD
PS51421RASSmall_GTPasePROSITE_PROFILES
PS51420RHOPROSITE_PROFILES
PS51419RABPROSITE_PROFILES
SM00174rho_sub_3Small_GTPaseSMART
SM00173ras_sub_4SMART
SM00175rab_sub_5SMART
SM00176ran_sub_2SMART
PR00449RASTRNSFRMNGPRINTS
G3DSA:3.40.50.300:FF:000573FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR24072RHO FAMILY GTPASESmall_GTPase_RhoPANTHER
TIGR00231small_GTPSmall_GTP-bdNCBIFAM

Orthologs

References / Literature

PMID:31666919 - Rho Family GTPases in Fission Yeast Cytokinesis.
Hercyk B et al. Commun Integr Biol 2019;12(1):171-180
PMID:27385337 - Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
Liu Y et al. Mol Biol Cell 2016 Aug 15;27(16):2528-41
PMID:20164182 - Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.
Soto T et al. J Biol Chem 2010 Apr 09;285(15):11516-25
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:39540318 - Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.
Moscoso-Romero E et al. Open Biol 2024 Nov;14(11):240101
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:28536259 - Differential functional regulation of protein kinase C (PKC) orthologs in fission yeast.
Madrid M et al. J Biol Chem 2017 Jul 07;292(27):11374-11387
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:7698654 - Isolation and sequencing of two cDNA clones encoding Rho proteins from the fission yeast Schizosaccharomyces pombe.
Nakano K et al. Gene 1995 Mar 21;155(1):119-22
PMID:27451356 - Skb5, an SH3 adaptor protein, regulates Pmk1 MAPK signaling by controlling the intracellular localization of the MAPKKK Mkh1.
Kanda Y et al. J Cell Sci 2016 Aug 15;129(16):3189-202
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:20624220 - Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast.
Cadou A et al. Mol Microbiol 2010 Sep;77(5):1186-202
PMID:32435206 - Posttranslational Arginylation Enzyme Arginyltransferase1 Shows Genetic Interactions With Specific Cellular Pathways in vivo .
Wiley DJ et al. Front Physiol 2020;11:427
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:26960792 - Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.
Revilla-Guarinos MT et al. Mol Biol Cell 2016 Mar 09;27(9):1524-35
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:24820419 - Rho2 palmitoylation is required for plasma membrane localization and proper signaling to the fission yeast cell integrity mitogen- activated protein kinase pathway.
Sánchez-Mir L et al. Mol Cell Biol 2014 Jul;34(14):2745-59
PMID:24928510 - Glucose activates TORC2-Gad8 protein via positive regulation of the cAMP/cAMP-dependent protein kinase A (PKA) pathway and negative regulation of the Pmk1 protein-mitogen-activated protein kinase pathway.
Cohen A et al. J Biol Chem 2014 Aug 01;289(31):21727-37
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:19486165 - Pleiotropic phenotypes caused by an opal nonsense mutation in an essential gene encoding HMG-CoA reductase in fission yeast.
Fang Y et al. Genes Cells 2009 Jun;14(6):759-71
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:11102532 - Schizosaccharomyces pombe rho2p GTPase regulates cell wall alpha-glucan biosynthesis through the protein kinase pck2p.
Calonge TM et al. Mol Biol Cell 2000 Dec;11(12):4393-401
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:14637153 - Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast.
Iwaki N et al. Biochem Biophys Res Commun 2003 Dec 12;312(2):414-20
PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.
Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007
PMID:17005909 - Rho2 is a target of the farnesyltransferase Cpp1 and acts upstream of Pmk1 mitogen-activated protein kinase signaling in fission yeast.
Ma Y et al. Mol Biol Cell 2006 Dec;17(12):5028-37
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:23738021 - A genome-wide screening of potential target genes to enhance the antifungal activity of micafungin in Schizosaccharomyces pombe.
Zhou X et al. PLoS One 2013;8(5):e65904
PMID:24498240 - Rho1 GTPase and PKC ortholog Pck1 are upstream activators of the cell integrity MAPK pathway in fission yeast.
Sánchez-Mir L et al. PLoS One 2014;9(1):e88020
PMID:37792890 - Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast.
Willet AH et al. PLoS Genet 2023 Oct;19(10):e1010987
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:16421249 - Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.
García P et al. Mol Biol Cell 2006 Apr;17(4):1620-31
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PB_REF:0000001 - Protein modification annotation by manual transfer of experimentally-verified annotation data to orthologs based on curator judgment of sequence features.
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:19037094 - The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.
Garcia P et al. Mol Biol Cell 2009 Jan;20(2):721-31
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:9405296 - Genes that cause aberrant cell morphology by overexpression in fission yeast: a role of a small GTP-binding protein Rho2 in cell morphogenesis.
Hirata D et al. J Cell Sci 1998 Jan;111 ( Pt 2):149-59
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.
Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81
PMID:10504305 - Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity.
Arellano M et al. J Cell Sci 1999 Oct;112 ( Pt 20):3569-78
PMID:23934882 - Negative functional interaction between cell integrity MAPK pathway and Rho1 GTPase in fission yeast.
Viana RA et al. Genetics 2013 Oct;195(2):421-32
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59