PomBase home

protein coding gene - ire1 (SPAC167.01) - serine/threonine protein kinase, sensor for unfolded proteins in the ER Ire1

Gene summary

Standard name
ire1
Systematic ID
SPAC167.01
Product
serine/threonine protein kinase, sensor for unfolded proteins in the ER Ire1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ppk4
UniProt ID
O94537
ORFeome ID
48/48B07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1559383..1562818 reverse strand

Annotation

GO biological process

GO:0036498 - IRE1-mediated unfolded protein response

References:

GO:0006397 - mRNA processing

References:

GO:0010628 - positive regulation of gene expression

References:

GO:0140501 - positive regulation of reticulophagy

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005789 - endoplasmic reticulum membrane

References:

GO molecular function

GO:0004674 - protein serine/threonine kinase activity

References:

GO:0004521 - RNA endonuclease activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0007446 - decreased reticulophagy during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0004166 - increased oxygen consumption during vegetative growth

References:

Genotypes:

FYPO:0001456 - increased RNA level during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0003118 - normal cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0003896 - normal mitochondrial morphology

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

Protein features

PBO:0111830 - PQQ repeat

Protein sequence feature

SO:0000418 - signal_peptide

References:

SO:0001812 - transmembrane_helix

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0005909 - abnormal RNA 3'-end processing during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0007447 - abolished reticulophagy during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0005285 - decreased cysteine-type endopeptidase activity

References:

Genotypes:

FYPO:0005910 - decreased protein level during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0001128 - decreased septation index

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001456 - increased RNA level during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0005283 - increased viability upon inositol starvation

References:

Genotypes:

FYPO:0000355 - normal endoplasmic reticulum morphology

References:

Genotypes:

FYPO:0001240 - normal growth on diamide

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0010035 - normal nuclear pore clustering during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0003840 - sensitive to carbendazim

References:

Genotypes:

FYPO:0000843 - sensitive to dithiothreitol

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0002167 - sensitive to ethyl methanesulfonate

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000112 - sensitive to sorbitol

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPfam
PF06479Ribonuc_2-5AKEN_domPfam
cd10422RNase_Ire1CDD
cd13982STKc_IRE1CDD
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE patterns
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE profiles
PS51392KENKEN_domPROSITE profiles
SM00220S_TKcProt_kinase_domSMART
SM00564PQQPQQ_b-propeller_rptSMART
SM00580PUGSMART
G3DSA:1.10.510.10:FF:001420CATH-FunFam
G3DSA:1.20.1440.180:FF:000002CATH-FunFam
G3DSA:3.30.200.20:FF:000077CATH-FunFam
G3DSA:1.10.510.10CATH-Gene3D
G3DSA:1.20.1440.180KEN_sfCATH-Gene3D
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfCATH-Gene3D
G3DSA:3.30.200.20CATH-Gene3D
SSF50998Quinoprotein_ADH-like_sfSUPERFAMILY
SSF56112Kinase-like_dom_sfSUPERFAMILY
PTHR13954IRE1/2-likePANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte
Signal PeptideSignal-Peptide-DeepTMHMM

Orthologs

References / Literature

PMID:23066509 - New twists in the unfolded protein response.
Cross BC et al. Elife 2012 Oct 15;1:e00243
PMID:39601909 - Fission yeast Bsd1 is required for ER stress response in Ire1 independent manner.
Mahapatra PP et al. Mol Biol Rep 2024 Nov 27;52(1):19
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23066505 - The unfolded protein response in fission yeast modulates stability of select mRNAs to maintain protein homeostasis.
Kimmig P et al. Elife 2012 Oct 15;1:e00048
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:27191590 - Fission Yeast SCYL1/2 Homologue Ppk32: A Novel Regulator of TOR Signalling That Governs Survival during Brefeldin A Induced Stress to Protein Trafficking.
Kowalczyk KM et al. PLoS Genet 2016 May;12(5):e1006041
PMID:32866412 - Epr1, a UPR-upregulated soluble autophagy receptor for reticulophagy.
Zhao D et al. Autophagy 2020 Nov;16(11):2112-2113
PMID:22876361 - Endoplasmic reticulum involvement in yeast cell death.
Austriaco N Front Oncol 2012;2:87
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.
Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493
PMID:33904404 - Protomer alignment modulates specificity of RNA substrate recognition by Ire1.
Li W et al. Elife 2021 Apr 27;10
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.
Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:32735772 - A UPR-Induced Soluble ER-Phagy Receptor Acts with VAPs to Confer ER Stress Resistance.
Zhao D et al. Mol Cell 2020 Sep 17;79(6):963-977.e3
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:38360270 - A KDELR-mediated ER-retrieval system modulates mitochondrial functions via the unfolded protein response in fission yeast.
Zhu M et al. J Biol Chem 2024 Feb 13;:105754
PMID:19606215 - Calnexin regulates apoptosis induced by inositol starvation in fission yeast.
Guérin R et al. PLoS One 2009 Jul 16;4(7):e6244
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22682253 - Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A et al. Cell 2012 Jun 08;149(6):1339-52
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20501954 - Search for kinases related to transition of growth polarity in fission yeast.
Koyano T et al. Biosci Biotechnol Biochem 2010;74(5):1129-33
GO_REF:0000002 - Comments
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:30110882 - The Unfolded Protein Response Pathway in the Yeast Kluyveromyces lactis . A Comparative View among Yeast Species.
Hernández-Elvira M et al. Cells 2018 Aug 14;7(8)
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22291963 - Histone chaperone Asf1 plays an essential role in maintaining genomic stability in fission yeast.
Tanae K et al. PLoS One 2012;7(1):e30472
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531