protein coding gene - rfc4 (SPAC1687.03c) - DNA replication factor C complex subunit Rfc4

Gene summary

Standard name: rfc4
Systematic ID: SPAC1687.03c
Product: DNA replication factor C complex subunit Rfc4
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O94449
ORFeome ID: 22/22B10
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome I: 905077..906395 reverse strand

Annotation

GO biological process

GO:1902983 - DNA strand elongation involved in mitotic DNA replication

References:

PMID:10748208

GO:1903460 - mitotic DNA replication leading strand elongation

References:

GO_REF:0000024

GO:0070914 - UV-damage excision repair

References:

PMID:10704216

GO cellular component

GO:0000785 - chromatin

References:

GO_REF:0000111

GO:0031390 - Ctf18 RFC-like complex

References:

GO_REF:0000033

GO:0005829 - cytosol

References:

PMID:16823372

GO:0031391 - Elg1 RFC-like complex

References:

PMID:16040599

GO:0043599 - nuclear DNA replication factor C complex

References:

GO_REF:0000033

GO:0005634 - nucleus

References:

PMID:16823372

GO:0031389 - Rad17 RFC-like complex

References:

GO_REF:0000033

GO molecular function

GO:0005524 - ATP binding

References:

GO_REF:0000002

GO:0016887 - ATP hydrolysis activity

References:

GO_REF:0000002

GO:0003677 - DNA binding

References:

GO_REF:0000002

GO:0003689 - DNA clamp loader activity

References:

GO_REF:0000033

GO:0061860 - DNA clamp unloader activity

References:

PMID:16040599

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:33823663

Protein features

PBO:0111792 - AAA family ATPase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0002148 - inviable after spore germination, multiple cell divisions, abnormal morphology

References:

PMID:23697806

Genotypes:

rfc4delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

rfc4delta

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF08542	Rep_fac_C	Rep_factorC_C	PFAM
PF00004	AAA	ATPase_AAA_core	PFAM
cd00009	AAA		CDD
cd18140	HLD_clamp_RFC	RFC_lid	CDD
SM00382	AAA_5	AAA+_ATPase	SMART
G3DSA:1.20.272.10:FF:000015			FUNFAM
G3DSA:3.40.50.300:FF:000107			FUNFAM
G3DSA:1.10.8.60:FF:000012			FUNFAM
SSF52540	P-loop containing nucleoside triphosphate hydrolases	P-loop_NTPase	SUPERFAMILY
SSF48019	post-AAA+ oligomerization domain-like	DNA_pol3_clamp-load_cplx_C	SUPERFAMILY
G3DSA:1.20.272.10			GENE3D
G3DSA:1.10.8.60			GENE3D
G3DSA:3.40.50.300		P-loop_NTPase	GENE3D
PTHR11669	REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT	DNA_Rep/Repair_Clamp_Loader	PANTHER
NF001679	PRK00440.1		NCBIFAM

Orthologs

RFC2 (HGNC:9970) Homo sapiens
rfc4 (SJAG_00729) Schizosaccharomyces japonicus
RFC4 (YOL094C) Saccharomyces cerevisiae

References / Literature

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:10748208 - Fidelity of eucaryotic DNA polymerase delta holoenzyme from Schizosaccharomyces pombe.: Chen X et al. J Biol Chem 2000 Jun 09;275(23):17677-82
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16040599 - Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.: Kim J et al. Nucleic Acids Res 2005;33(13):4078-89
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18045993 - RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombe.: Ansbach AB et al. Mol Biol Cell 2008 Feb;19(2):595-607
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.: Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000002 - Comments
PMID:10704216 - In vitro reconstitution of the Schizosaccharomyces pombe alternative excision repair pathway.: Alleva JL et al. Biochemistry 2000 Mar 14;39(10):2659-66
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.: Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053