PomBase home

protein coding gene - csn5 (SPAC1687.13c) - COP9/signalosome complex protease subunit Csn5

Gene summary

Standard name
csn5
Systematic ID
SPAC1687.13c
Product
COP9/signalosome complex protease subunit Csn5
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94454
ORFeome ID
16/16E01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 926394..927626 reverse strand

Annotation

GO biological process

GO:0000338 - protein deneddylation

References:

GO cellular component

GO:0008180 - COP9 signalosome

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0019784 - deNEDDylase activity

References:

GO:0008237 - metallopeptidase activity

References:

GO:0005515 - protein binding

References:

Multi-locus phenotype

FYPO:0002553 - abnormal double-strand break processing

References:

Genotypes:

FYPO:0005822 - decreased NEDD8-specific protease activity

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0005820 - increased protein neddylation during vegetative growth

References:

Genotypes:

FYPO:0001571 - increased protein-protein interaction

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0002800 - normal protein degradation during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009039 - resistance to potassium chloride

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01398JABJAMM/MPN+_domPFAM
cd08069MPN_RPN11_CSN5CDD
PS50249MPNMPNPROSITE_PROFILES
SM00232pad1_6JAMM/MPN+_domSMART
G3DSA:3.40.140.10:FF:000203FUNFAM
SSF102712JAB1/MPN domainSUPERFAMILY
G3DSA:3.40.140.10Cytidine Deaminase, domain 2GENE3D
PTHR10410EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATEDJAMM_MPN+_peptidase_M67APANTHER

Orthologs

References / Literature

PMID:16252005 - Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase.
Liu C et al. EMBO J 2005 Nov 16;24(22):3940-51
PMID:15769255 - Nep1, a Schizosaccharomyces pombe deneddylating enzyme.
Zhou L et al. Biochem J 2005 Jul 15;389(Pt 2):307-14
PMID:12183637 - Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1.
Cope GA et al. Science 2002 Oct 18;298(5593):608-11
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:11854407 - Deletion mutants in COP9/signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes.
Mundt KE et al. Mol Biol Cell 2002 Feb;13(2):493-502
PMID:23496905 - Nedd8 processing enzymes in Schizosaccharomyces pombe.
O'Donoghue JE et al. BMC Biochem 2013 Mar 15;14:8
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:12718879 - Fission yeast COP9/signalosome suppresses cullin activity through recruitment of the deubiquitylating enzyme Ubp12p.
Zhou C et al. Mol Cell 2003 Apr;11(4):927-38
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:11504566 - The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p.
Zhou C et al. BMC Biochem 2001;2:7
PMID:19748355 - F-box-directed CRL complex assembly and regulation by the CSN and CAND1.
Schmidt MW et al. Mol Cell 2009 Sep 11;35(5):586-97
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27098497 - CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.
Zeng M et al. Nat Commun 2016 Apr 21;7:11364
PMID:15793566 - CSN facilitates Cullin-RING ubiquitin ligase function by counteracting autocatalytic adapter instability.
Wee S et al. Nat Cell Biol 2005 Apr;7(4):387-91
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23535663 - CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire.
Wu S et al. Nat Commun 2013;4:1642
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:22673520 - Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe.
Girdwood D et al. FEBS Lett 2012 May 21;586(10):1522-8
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12