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protein coding gene - ssl3 (SPAC1687.18c) - cohesin loading factor Ssl3

Gene summary

Standard name
ssl3
Systematic ID
SPAC1687.18c
Product
cohesin loading factor Ssl3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94459
ORFeome ID
36/36G07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 936188..938118 reverse strand

Annotation

GO biological process

GO:0140588 - chromatin looping

References:

GO:0007064 - mitotic sister chromatid cohesion

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0090694 - Scc2-Scc4 cohesin loading complex

References:

GO:0032116 - SMC loading complex

References:

GO molecular function

GO:0061775 - cohesin loader activity

References:

GO:0003690 - double-stranded DNA binding

References:

GO:0005515 - protein binding

References:

Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0005345 - mitotic sister chromatid separation during interphase

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111755 - TPR repeat protein

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000450 - decreased protein localization to centromere during vegetative growth

References:

Genotypes:

FYPO:0005386 - decreased protein localization to chromatin at chromosome arms

References:

Genotypes:

FYPO:0001514 - decreased protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0001509 - normal protein localization to chromatin during vegetative growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10345Cohesin_loadMAU2PFAM
PTHR21394MAU2 CHROMATID COHESION FACTOR HOMOLOGMAU2PANTHER

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26687354 - DNA Entry into and Exit out of the Cohesin Ring by an Interlocking Gate Mechanism.
Murayama Y et al. Cell 2015 Dec 17;163(7):1628-40
PMID:16682348 - A screen for cohesion mutants uncovers Ssl3, the fission yeast counterpart of the cohesin loading factor Scc4.
Bernard P et al. Curr Biol 2006 May 09;16(9):875-81
PMID:31895039 - The CDK Pef1 and protein phosphatase 4 oppose each other for regulating cohesin binding to fission yeast chromosomes.
Birot A et al. Elife 2020 Jan 02;9
PMID:18079700 - Cell-cycle regulation of cohesin stability along fission yeast chromosomes.
Bernard P et al. EMBO J 2008 Jan 09;27(1):111-21
PMID:19454013 - Conserved features of cohesin binding along fission yeast chromosomes.
Schmidt CK et al. Genome Biol 2009;10(5):R52
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33176147 - DNA Binding by the Mis4 Scc2 Loader Promotes Topological DNA Entrapment by the Cohesin Ring.
Kurokawa Y et al. Cell Rep 2020 Nov 10;33(6):108357
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:29358048 - Establishment of DNA-DNA Interactions by the Cohesin Ring.
Murayama Y et al. Cell 2018 Jan 25;172(3):465-477.e15
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26583750 - The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering.
Çamdere G et al. Elife 2015 Nov 19;4
PMID:36509793 - Functional crosstalk between the cohesin loader and chromatin remodelers.
Muñoz S et al. Nat Commun 2022 Dec 13;13(1):7698
PMID:40193710 - An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.
Wang YT et al. Nucleic Acids Res 2025 Mar 20;53(6)
PMID:34309513 - A Brownian ratchet model for DNA loop extrusion by the cohesin complex.
Higashi TL et al. Elife 2021 Jul 26;10
InterPro:IPR011990 - Unknown title
PMID:24291789 - Biochemical reconstitution of topological DNA binding by the cohesin ring.
Murayama Y et al. Nature 2014 Jan 16;505(7483):367-71
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:32277274 - Conserved roles of chromatin remodellers in cohesin loading onto chromatin.
Muñoz S et al. Curr Genet 2020 Oct;66(5):951-956
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623