protein coding gene - mis6 (SPAC1687.20c) - CENP-I ortholog, inner centromere connector Mis6

Gene summary

Standard name

mis6

Systematic ID

SPAC1687.20c

Product

CENP-I ortholog, inner centromere connector Mis6

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P87227

ORFeome ID

27/27E07

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 939770..941944 reverse strand

Annotation

Protein features

Orthologs

• CENPI (HGNC:3968) Homo sapiens
• mis6 (SJAG_00684) Schizosaccharomyces japonicus
• CTF3 (YLR381W) Saccharomyces cerevisiae

References / Literature

PMID:30967422 - Negative Regulation of the Mis17-Mis6 Centromere Complex by mRNA Decay Pathway and EKC/KEOPS Complex in Schizosaccharomyces pombe .

Xu X et al. G3 (Bethesda) 2019 Jun 05;9(6):1815-1823

PMID:24497846 - A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.

Kriegenburg F et al. PLoS Genet 2014 Jan;10(1):e1004140

PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.

Williams JS et al. Mol Cell 2009 Feb 13;33(3):287-98

PMID:10864871 - Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.

Takahashi K et al. Science 2000 Jun 23;288(5474):2215-9

PMID:24774534 - Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex.

Hayashi T et al. Genes Cells 2014 Jul;19(7):541-54

PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.

Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307

PMID:11780129 - Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast.

Nonaka N et al. Nat Cell Biol 2002 Jan;4(1):89-93

PMID:26275423 - Inner Kinetochore Protein Interactions with Regional Centromeres of Fission Yeast.

Thakur J et al. Genetics 2015 Oct;201(2):543-61

PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .

Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080

PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.

Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43

PMID:28827290 - Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe .

Gao C et al. Genetics 2017 Oct;207(2):559-569

PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.

Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77

PMID:10766735 - Distinct protein interaction domains and protein spreading in a complex centromere.

Partridge JF et al. Genes Dev 2000 Apr 01;14(7):783-91

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24789708 - Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly.

Subramanian L et al. Open Biol 2014 Apr 30;4(4):140043

PMID:16688222 - Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation.

Maruyama T et al. EMBO J 2006 Jun 07;25(11):2420-31

PMID:23091701 - Impaired coenzyme A synthesis in fission yeast causes defective mitosis, quiescence-exit failure, histone hypoacetylation and fragile DNA.

Nakamura T et al. Open Biol 2012 Sep;2(9):120117

PMID:25619765 - The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast.

Folco HD et al. Curr Biol 2015 Feb 02;25(3):348-356

PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.

Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54

PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.

Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30

PMID:11553715 - The domain structure of centromeres is conserved from fission yeast to humans.

Kniola B et al. Mol Biol Cell 2001 Sep;12(9):2767-75

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:39786922 - The fission yeast SUMO-targeted ubiquitin ligase Slx8 functionally associates with clustered centromeres and the silent mating-type region at the nuclear periphery.

Chakraborty S et al. Biol Open 2024 Dec 15;13(12)

PMID:16855021 - Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein.

Kerres A et al. Mol Biol Cell 2006 Oct;17(10):4167-78

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:10398680 - Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation.

Goshima G et al. Genes Dev 1999 Jul 01;13(13):1664-77

PMID:15728720 - Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast.

Asakawa H et al. Mol Biol Cell 2005 May;16(5):2325-38

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.

Hirai H et al. Commun Biol 2022 Aug 15;5(1):818

PMID:25764183 - Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast.

Xu X et al. PLoS One 2015;10(3):e0119347

PMID:18493607 - A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.

Natsume T et al. PLoS One 2008 May 21;3(5):e2221

PMID:34460892 - Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis.

Tarhan Ç et al. Genet Mol Biol 2021;44(3):e20200245

PMID:34849791 - Perturbation of kinetochore function using GFP-binding protein in fission yeast.

Deng DJ et al. G3 (Bethesda) 2021 Oct 19;11(11)

PMID:23267073 - Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.

Gonzalez M et al. Proc Natl Acad Sci U S A 2013 Jan 08;110(2):606-11

PMID:19217404 - Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.

Pidoux AL et al. Mol Cell 2009 Feb 13;33(3):299-311

PMID:7865880 - Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.

Takahashi K et al. Mol Biol Cell 1994 Oct;5(10):1145-58

PMID:27889481 - Mitotic Nuclear Envelope Breakdown and Spindle Nucleation Are Controlled by Interphase Contacts between Centromeres and the Nuclear Envelope.

Fernández-Álvarez A et al. Dev Cell 2016 Dec 05;39(5):544-559

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:17895368 - Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth.

Alvarez-Tabarés I et al. J Cell Sci 2007 Oct 15;120(Pt 20):3589-601

PMID:9230309 - Mis6, a fission yeast inner centromere protein, acts during G1/S and forms specialized chromatin required for equal segregation.

Saitoh S et al. Cell 1997 Jul 11;90(1):131-43

PMID:21445296 - Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast.

Shiroiwa Y et al. PLoS One 2011 Mar 21;6(3):e17761

PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.

Hayashi T et al. Cell 2004 Sep 17;118(6):715-29

PMID:12928332 - Distinct centromere domain structures with separate functions demonstrated in live fission yeast cells.

Appelgren H et al. J Cell Sci 2003 Oct 01;116(Pt 19):4035-42

PMID:17947424 - Msc1 acts through histone H2A.Z to promote chromosome stability in Schizosaccharomyces pombe.

Ahmed S et al. Genetics 2007 Nov;177(3):1487-97

PMID:23427262 - The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.

Tang NH et al. Mol Biol Cell 2013 Apr;24(8):1122-33

PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.

Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928

PMID:18362178 - Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis.

Nakazawa N et al. J Cell Biol 2008 Mar 24;180(6):1115-31

PMID:22065639 - Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation.

Chen JS et al. J Cell Biol 2011 Nov 14;195(4):583-93

PMID:12535531 - A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast.

Chen ES et al. Mol Cell 2003 Jan;11(1):175-87

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:17352737 - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins.

Kobayashi Y et al. Genes Cells 2007 Mar;12(3):311-28

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:25375240 - The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A.

Hirai H et al. PLoS One 2014;9(11):e111905

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:39378339 - Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability.

Ishikawa K et al. J Cell Biol 2025 Jan 06;224(1)

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:15908586 - The CHD remodeling factor Hrp1 stimulates CENP-A loading to centromeres.

Walfridsson J et al. Nucleic Acids Res 2005;33(9):2868-79

PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.

Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84

PMID:28455357 - Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase.

Lin L et al. Biol Open 2017 Jun 15;6(6):844-849

PMID:23028377 - Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast.

Choi ES et al. PLoS Genet 2012 Sep;8(9):e1002985