protein coding gene - tfb1 (SPAC16E8.11c) - transcription factor TFIIH complex subunit Tfb1

Gene summary

Standard name: tfb1
Systematic ID: SPAC16E8.11c
Product: transcription factor TFIIH complex subunit Tfb1
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O13745
ORFeome ID: 25/25D09
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome I: 3520173..3522389 reverse strand

Annotation

GO biological process

GO:0006289 - nucleotide-excision repair

References:

GO_REF:0000024

GO:0006360 - transcription by RNA polymerase I

References:

GO_REF:0000033

GO:0006367 - transcription initiation at RNA polymerase II promoter

References:

GO cellular component

GO:0005737 - cytoplasm

References:

PMID:16823372

GO:0005829 - cytosol

References:

PMID:16823372

GO:0000112 - nucleotide-excision repair factor 3 complex

References:

GO_REF:0000024

GO:0000439 - transcription factor TFIIH core complex

References:

PMID:15182371

GO:0005675 - transcription factor TFIIH holo complex

References:

GO_REF:0000033

GO molecular function

GO:0016251 - RNA polymerase II general transcription initiation factor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:39705284

MOD:01148 - ubiquitinylated lysine

References:

PMID:26412298

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

PMID:32101745

Genotypes:

tfb1::Tn-seq

FYPO:0002515 - inviable after spore germination, without cell division, with branched, elongated germ tube

References:

PMID:23697806

Genotypes:

tfb1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

tfb1delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

PMID:21511999

Protein features

ID	Name	InterPro name	DB name
PF03909	BSD	BSD_dom	PFAM
PF08567	PH_TFIIH	TFIIH_BTF_p62_N	PFAM
cd13229	PH_TFIIH		CDD
PS50858	BSD	BSD_dom	PROSITE_PROFILES
SM00751	wurzfinal6	BSD_dom	SMART
SSF50729	PH domain-like		SUPERFAMILY
SSF140383	BSD domain-like	BSD_dom_sf	SUPERFAMILY
G3DSA:2.30.29.30		PH-like_dom_sf	GENE3D
G3DSA:6.10.140.1200			GENE3D
PTHR12856	TRANSCRIPTION INITIATION FACTOR IIH-RELATED	Tfb1/GTF2H1	PANTHER
mobidb-lite-Disorder	disorder_prediction		MOBIDB-Disorder
mobidb-lite-Polar	disorder_prediction		MOBIDB-Polar

Orthologs

GTF2H1 (HGNC:4655) Homo sapiens
tfb1 (SJAG_00512) Schizosaccharomyces japonicus
TFB1 (YDR311W) Saccharomyces cerevisiae

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:37340985 - Structural polymorphism of the PH domain in TFIIH.: Okuda M et al. Biosci Rep 2023 Jul 26;43(7)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22540037 - Predicting the fission yeast protein interaction network.: Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:14534314 - Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro.: Spahr H et al. J Biol Chem 2003 Dec 19;278(51):51301-6
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.: Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.: Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.: Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:8303296 - RNA polymerase II initiation factor interactions and transcription start site selection.: Li Y et al. Science 1994 Feb 11;263(5148):805-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.: Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.: Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.: Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41