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protein coding gene - tif45 (SPAC16E8.15) - translation initiation factor eIF4E, 4F complex subunit

Gene summary

Standard name
tif45
Systematic ID
SPAC16E8.15
Product
translation initiation factor eIF4E, 4F complex subunit
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
tif1
UniProt ID
P78954
ORFeome ID
11/11D12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3528153..3529446 forward strand

Annotation

Disease association

MONDO:0014041 - autism, susceptibility to, 19

References:

GO biological process

GO:0002183 - cytoplasmic translational initiation

References:

GO:0000184 - nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

References:

GO cellular component

GO:0010494 - cytoplasmic stress granule

References:

GO:0005829 - cytosol

References:

GO:0016281 - eukaryotic translation initiation factor 4F complex

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0000340 - RNA 7-methylguanosine cap binding

References:

GO:0003743 - translation initiation factor activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001042 - inviable after spore germination, single or double cell division

References:

Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003116 - sensitive to plumbagin

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01652IF4ETIF_eIF_4EPFAM
PS00813IF4ETIF_eIF_4E_CSPROSITE_PATTERNS
G3DSA:3.30.760.10:FF:000004FUNFAM
SSF55418eIF4e-likeTIF_eIF4e-likeSUPERFAMILY
G3DSA:3.30.760.10RNA Cap, Translation Initiation Factor Eif4eTIF_eIF4e-likeGENE3D
PTHR11960EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATEDTIF_eIF_4EPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:10462529 - Translational control of the cdc25 cell cycle phosphatase: a molecular mechanism coupling mitosis to cell growth.
Daga RR et al. J Cell Sci 1999 Sep;112 Pt 18:3137-46
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23028742 - The natural anticancer agent plumbagin induces potent cytotoxicity in MCF-7 human breast cancer cells by inhibiting a PI-5 kinase for ROS generation.
Lee JH et al. PLoS One 2012;7(9):e45023
PMID:12581158 - Overproduction of a conserved domain of fission yeast and mammalian translation initiation factor eIF4G causes aberrant cell morphology and results in disruption of the localization of F-actin and the organization of microtubules.
Hashemzadeh-Bonehi L et al. Genes Cells 2003 Feb;8(2):163-78
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:31822915 - Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.
Taglini F et al. Nucleic Acids Res 2020 Feb 20;48(3):1239-1253
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:8955119 - Schizosaccharomyces pombe has a novel eukaryotic initiation factor 4F complex containing a cap-binding protein with the human eIF4E C-terminal motif KSGST.
Ptushkina M et al. J Biol Chem 1996 Dec 20;271(51):32818-24
PMID:14968130 - eIF4E isoform 2 in Schizosaccharomyces pombe is a novel stress-response factor.
Ptushkina M et al. EMBO Rep 2004 Mar;5(3):311-6
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:32075773 - Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress.
Cabrera M et al. Cell Rep 2020 Feb 18;30(7):2430-2443.e4
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671