protein coding gene - clp1 (SPAC1782.09c) - serine/threonine protein phosphatase (Cdc14-related) Clp1/Flp1

Gene summary

Standard name

clp1

Systematic ID

SPAC1782.09c

Product

serine/threonine protein phosphatase (Cdc14-related) Clp1/Flp1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

flp1

UniProt ID

Q9P7H1

ORFeome ID

45/45E07

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 4769991..4771716 reverse strand

Annotation

Protein features

Orthologs

• CDC14A (HGNC:1718) Homo sapiens
• CDC14B (HGNC:1719) Homo sapiens
• clp1 (SJAG_00082) Schizosaccharomyces japonicus
• CDC14 (YFR028C) Saccharomyces cerevisiae

References / Literature

PMID:19686686 - Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation.

Fu C et al. Dev Cell 2009 Aug;17(2):257-67

PMID:16687577 - Cell cycle-dependent roles for the FCH-domain protein Cdc15p in formation of the actomyosin ring in Schizosaccharomyces pombe.

Wachtler V et al. Mol Biol Cell 2006 Jul;17(7):3254-66

PMID:29343550 - Cdk1-dependent phosphoinhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring formation.

Willet AH et al. Mol Biol Cell 2018 Mar 15;29(6):713-721

PMID:21098641 - Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p.

Papadopoulou K et al. J Cell Sci 2010 Dec 15;123(Pt 24):4374-81

PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.

Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433

PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.

Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99

PMID:16049679 - Mpg1, a fission yeast protein required for proper septum structure, is involved in cell cycle progression through cell-size checkpoint.

Donoso I et al. Mol Genet Genomics 2005 Sep;274(2):155-67

PMID:21931816 - Global gene expression analysis of fission yeast mutants impaired in Ser-2 phosphorylation of the RNA pol II carboxy terminal domain.

Saberianfar R et al. PLoS One 2011;6(9):e24694

PMID:22375062 - Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.

Buttrick GJ et al. J Cell Sci 2012 Apr 01;125(Pt 7):1645-51

PMID:24115772 - SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis.

Bohnert KA et al. Genes Dev 2013 Oct 01;27(19):2164-77

PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.

Materne P et al. Elife 2015 Jun 22;4:e09008

PMID:22624651 - A systematic screen reveals new elements acting at the G2/M cell cycle control.

Navarro FJ et al. Genome Biol 2012 May 24;13(5):R36

PMID:34080538 - Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.

Krüger LK et al. Elife 2021 Jun 03;10

PMID:19523829 - Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation.

Choi SH et al. Curr Biol 2009 Jun 23;19(12):985-95

PMID:18272786 - Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast.

Ge W et al. Mol Biol Cell 2008 Apr;19(4):1680-92

PMID:34133210 - Cdk1 phosphorylation of fission yeast paxillin inhibits its cytokinetic ring localization.

Mangione MC et al. Mol Biol Cell 2021 Aug 15;32(17):1534-1544

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:17502918 - A cyclin-dependent kinase that promotes cytokinesis through modulating phosphorylation of the carboxy terminal domain of the RNA Pol II Rpb1p sub-unit.

Karagiannis J et al. PLoS One 2007 May 09;2(5):e433

PMID:22665807 - Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches.

Lu LX et al. Proc Natl Acad Sci U S A 2012 Jun 19;109(25):9899-904

PMID:15265986 - The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe.

Mishra M et al. J Cell Sci 2004 Aug 01;117(Pt 17):3897-910

PMID:19139265 - The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

Roberts-Galbraith RH et al. J Cell Biol 2009 Jan 12;184(1):113-27

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:15525536 - The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase.

Trautmann S et al. Dev Cell 2004 Nov;7(5):755-62

PMID:15128870 - A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast.

Esteban V et al. J Cell Sci 2004 May 15;117(Pt 12):2461-8

PMID:37128864 - CDK actively contributes to establishment of the stationary phase state in fission yeast.

Hiraoka M et al. J Cell Sci 2023 May 15;136(10)

PMID:18378776 - The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1.

Clifford DM et al. J Cell Biol 2008 Apr 07;181(1):79-88

PMID:12479804 - Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN).

Guertin DA et al. Dev Cell 2002 Dec;3(6):779-90

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:17538026 - The nucleolar Net1/Cfi1-related protein Dnt1 antagonizes the septation initiation network in fission yeast.

Jin QW et al. Mol Biol Cell 2007 Aug;18(8):2924-34

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:16085489 - The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast.

Mishra M et al. Curr Biol 2005 Aug 09;15(15):1376-83

PMID:12546793 - Mitotic hyperphosphorylation of the fission yeast SIN scaffold protein cdc11p is regulated by the protein kinase cdc7p.

Krapp A et al. Curr Biol 2003 Jan 21;13(2):168-72

PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.

Ma Y et al. PLoS One 2011;6(8):e23422

PMID:23394829 - Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways.

Gupta S et al. Curr Biol 2013 Feb 18;23(4):333-8

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:15537703 - The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe.

Karagiannis J et al. Mol Biol Cell 2005 Jan;16(1):358-71

PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Mak T et al. EMBO J 2021 Aug 16;40(16):e107911

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:22737087 - The CCR4-NOT complex is implicated in the viability of aneuploid yeasts.

Tange Y et al. PLoS Genet 2012;8(6):e1002776

PMID:27749909 - A New Membrane Protein Sbg1 Links the Contractile Ring Apparatus and Septum Synthesis Machinery in Fission Yeast.

Sethi K et al. PLoS Genet 2016 Oct;12(10):e1006383

PMID:15857958 - Roles of Pdk1p, a fission yeast protein related to phosphoinositide-dependent protein kinase, in the regulation of mitosis and cytokinesis.

Bimbó A et al. Mol Biol Cell 2005 Jul;16(7):3162-75

PMID:16950131 - Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe.

Wolfe BA et al. Dev Cell 2006 Sep;11(3):423-30

PMID:18385517 - Cds1 controls the release of Cdc14-like phosphatase Flp1 from the nucleolus to drive full activation of the checkpoint response to replication stress in fission yeast.

Díaz-Cuervo H et al. Mol Biol Cell 2008 Jun;19(6):2488-99

PMID:20967237 - Fission yeast cells undergo nuclear division in the absence of spindle microtubules.

Castagnetti S et al. PLoS Biol 2010 Oct 12;8(10):e1000512

PMID:11448769 - Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression.

Trautmann S et al. Curr Biol 2001 Jun 26;11(12):931-40

PMID:14765109 - Fission yeast Clp1p phosphatase affects G2/M transition and mitotic exit through Cdc25p inactivation.

Wolfe BA et al. EMBO J 2004 Feb 25;23(4):919-29

PMID:22918952 - Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress.

Broadus MR et al. Mol Biol Cell 2012 Oct;23(20):4118-28

PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.

Bernal M et al. Proteomics 2014 Jun;14(11):1367-80

PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7

PMID:32361273 - Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.

Stonyte V et al. iScience 2020 May 22;23(5):101063

PMID:35108037 - Fission yeast paxillin contains two Cdc15 binding motifs for robust recruitment to the cytokinetic ring.

Snider CE et al. Mol Biol Cell 2022 Apr 01;33(4):br4

PMID:38415071 - The Cdc14 phosphatase, Clp1, does not affect genome expression.

Lopez Maury L et al. MicroPubl Biol 2024;2024

PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.

Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:11683392 - Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis.

Cueille N et al. J Cell Sci 2001 Jul;114(Pt 14):2649-64

PMID:28103117 - Wee1 and Cdc25 are controlled by conserved PP2A-dependent mechanisms in fission yeast.

Lucena R et al. Cell Cycle 2017 Mar 04;16(5):428-435

PMID:15911625 - Functional homology among human and fission yeast Cdc14 phosphatases.

Vázquez-Novelle MD et al. J Biol Chem 2005 Aug 12;280(32):29144-50

PMID:18418059 - The Flp1/Clp1 phosphatase cooperates with HECT-type Pub1/2 protein-ubiquitin ligases in Schizosaccharomyces pombe.

Esteban V et al. Cell Cycle 2008 May 01;7(9):1269-76

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.

Blyth J et al. Genetics 2018 Feb;208(2):589-603

PMID:33176152 - Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast.

Gallardo P et al. Cell Rep 2020 Nov 10;33(6):108377

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:18951025 - The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1.

Chen CT et al. Curr Biol 2008 Oct 28;18(20):1594-9

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:22347452 - The SET domain protein, Set3p, promotes the reliable execution of cytokinesis in Schizosaccharomyces pombe.

Rentas S et al. PLoS One 2012;7(2):e31224

PMID:21965289 - Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.

Buttrick GJ et al. Mol Biol Cell 2011 Dec;22(23):4486-502

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:17627824 - The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation.

Gregan J et al. Curr Biol 2007 Jul 17;17(14):1190-200

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:25356547 - A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe.

Bhutta MS et al. PLoS One 2014;9(10):e111789

PMID:24790095 - Characterization of the roles of Blt1p in fission yeast cytokinesis.

Goss JW et al. Mol Biol Cell 2014 Jul 01;25(13):1946-57

PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Anver S et al. EMBO Rep 2014 Aug;15(8):894-902

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:22559741 - A conserved histone deacetylase with a role in the regulation of cytokinesis in Schizosaccharomyces pombe.

Grewal C et al. Cell Div 2012 May 04;7(1):13

PMID:22065639 - Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation.

Chen JS et al. J Cell Biol 2011 Nov 14;195(4):583-93

PMID:18303049 - Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network.

Dischinger S et al. J Cell Sci 2008 Mar 15;121(Pt 6):843-53

PMID:16085490 - Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation.

Trautmann S et al. Curr Biol 2005 Aug 09;15(15):1384-9

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:22645648 - The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe.

Ikai N et al. Open Biol 2011 Nov;1(3):110007

PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.

McDonald NA et al. Elife 2017 Sep 15;6

PMID:21098635 - Mid1p-dependent regulation of the M-G1 transcription wave in fission yeast.

Agarwal M et al. J Cell Sci 2010 Dec 15;123(Pt 24):4366-73

PMID:25963819 - Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.

Lucena R et al. J Cell Biol 2015 May 11;209(3):387-402

PMID:26702831 - Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis.

McDonald NA et al. Dev Cell 2015 Dec 21;35(6):725-36

PMID:22264609 - A role for metaphase spindle elongation forces in correction of merotelic kinetochore attachments.

Choi SH et al. Curr Biol 2012 Feb 07;22(3):225-30

PMID:17032733 - S. pombe FEAR protein orthologs are not required for release of Clp1/Flp1 phosphatase from the nucleolus during mitosis.

Chen CT et al. J Cell Sci 2006 Nov 01;119(Pt 21):4462-6

PMID:29162650 - Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast.

Palani S et al. J Cell Sci 2018 Jan 04;131(1)

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7