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protein coding gene - hst4 (SPAC1783.04c) - histone deacetylase, Sirtuin family, NAD-dependent Hst4

Gene summary

Standard name
hst4
Systematic ID
SPAC1783.04c
Product
histone deacetylase, Sirtuin family, NAD-dependent Hst4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UR39
ORFeome ID
21/21C06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2192037..2193615 reverse strand

Annotation

GO biological process

GO:0140861 - DNA repair-dependent chromatin remodeling

References:

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO:0031508 - pericentric heterochromatin formation

References:

GO:0000183 - rDNA heterochromatin formation

References:

GO:0031509 - subtelomeric heterochromatin formation

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0099115 - chromosome, subtelomeric region

References:

GO:0031934 - mating-type region heterochromatin

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0005721 - pericentric heterochromatin

References:

GO:0033553 - rDNA heterochromatin

References:

GO molecular function

GO:0032041 - histone H3K14 deacetylase activity, NAD-dependent

References:

GO:0140765 - histone H3K56 deacetylase activity, NAD-dependent

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000013 - RNA level unchanged

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0005268 - abnormal mitotic cell cycle regulation during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0004492 - abnormal RNA 5'-end processing

References:

Genotypes:

FYPO:0000502 - abnormally arrested mitotic cell cycle progression

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Genotypes:

FYPO:0007879 - abolished protein degradation during cellular response to methyl methanesulfonate

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Genotypes:

FYPO:0004660 - abolished protein phosphorylation during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0000912 - abolished protein ubiquitination during vegetative growth

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003411 - decreased chromatin silencing at centromere inner repeat

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0004240 - decreased histone H3-K56 acetylation during vegetative growth

References:

Genotypes:

FYPO:0004491 - decreased nucleosome occupancy

References:

Genotypes:

FYPO:0007878 - decreased protein degradation during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0002909 - decreased protein localization to chromatin during vegetative growth

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Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0007255 - decreased replication fork processing

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Genotypes:

FYPO:0005545 - decreased Tf body formation

References:

Genotypes:

FYPO:0007270 - decreased Tf body tethering to centromere

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0004928 - fragmented DNA during vegetative growth

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Genotypes:

FYPO:0008162 - increased activation of S-phase DNA damage checkpoint

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0007881 - increased duration of histone H2A phosphorylation during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0008270 - increased H3-K56 acetylation during vegetative growth

References:

Genotypes:

FYPO:0000332 - increased histone acetylation during vegetative growth

References:

Genotypes:

FYPO:0004489 - increased level of amino acid biosynthesis gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0001861 - increased minichromosome loss upon segregation during vegetative growth

References:

Genotypes:

FYPO:0004189 - increased protein level during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0000239 - increased transcription from MCB promoter

References:

Genotypes:

FYPO:0004672 - inviable elongated vegetative cell with fragmented nucleus

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Genotypes:

FYPO:0007142 - loss of viability following cellular response to ionizing radiation

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001925 - normal cellular response to gamma radiation

References:

Genotypes:

FYPO:0002336 - normal chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0010017 - normal histone H2B-K33 acetylation during vegetative growth

References:

Genotypes:

FYPO:0008160 - normal histone H3-K56 acetylation during vegetative growth

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Genotypes:

FYPO:0003223 - normal histone H3-K9 acetylation during vegetative growth

References:

Genotypes:

FYPO:0008161 - normal histone H4-K16 acetylation during vegetative growth

References:

Genotypes:

FYPO:0007880 - normal protein degradation during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0007882 - normal protein kinase activity during cellular response to methyl methanesulfonate

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0003481 - viable elongated vegetative cell, elongated upon mitotic entry

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF02146SIR2SirtuinPfam
cd01407SIR2-famCDD
PS50305SIRTUINSsirtuin_cat_domPROSITE profiles
G3DSA:3.30.1600.10Sirtuin_cat_small_dom_sfCATH-Gene3D
G3DSA:3.40.50.1220CATH-Gene3D
SSF52467DHS-like_NAD/FAD-binding_domSUPERFAMILY
PTHR11085NAD-dep_sirtuin_deacylasesPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder

Orthologs

References / Literature

PMID:31262821 - Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks.
Feng G et al. Proc Natl Acad Sci U S A 2019 Jul 16;116(29):14563-14572
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:17446861 - Specific functions for the fission yeast Sirtuins Hst2 and Hst4 in gene regulation and retrotransposon silencing.
Durand-Dubief M et al. EMBO J 2007 May 16;26(10):2477-88
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23084836 - Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation.
Tanaka A et al. Mol Cell 2012 Nov 30;48(4):532-46
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:18344406 - Schizosaccharomyces pombe Hst4 functions in DNA damage response by regulating histone H3 K56 acetylation.
Haldar D et al. Eukaryot Cell 2008 May;7(5):800-13
PMID:23195958 - Anaphase-promoting complex/cyclosome-mediated proteolysis of Ams2 in the G1 phase ensures the coupling of histone gene expression to DNA replication in fission yeast.
Trickey M et al. J Biol Chem 2013 Jan 11;288(2):928-37
PMID:20299449 - A chromodomain switch mediated by histone H3 Lys 4 acetylation regulates heterochromatin assembly.
Xhemalce B et al. Genes Dev 2010 Apr 01;24(7):647-52
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.
Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
PMID:29855479 - Fission Yeast Sirtuin Hst4 Functions in Preserving Genomic Integrity by Regulating Replisome Component Mcl1.
Konada L et al. Sci Rep 2018 May 31;8(1):8496
PMID:21110984 - The role of MutY homolog (Myh1) in controlling the histone deacetylase Hst4 in the fission yeast Schizosaccharomyces pombe.
Chang DY et al. J Mol Biol 2011 Jan 21;405(3):653-65
PMID:31260531 - Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave.
González-Medina A et al. Nucleic Acids Res 2019 Sep 19;47(16):8439-8451
PMID:34608864 - DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks.
Aricthota S et al. Elife 2021 Oct 05;10
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
PMID:23091701 - Impaired coenzyme A synthesis in fission yeast causes defective mitosis, quiescence-exit failure, histone hypoacetylation and fragile DNA.
Nakamura T et al. Open Biol 2012 Sep;2(9):120117
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:20194963 - Transformation/transcription domain-associated protein (TRRAP)-mediated regulation of Wee1.
Calonge TM et al. Genetics 2010 May;185(1):81-93
PMID:10512858 - The Schizosaccharomyces pombe hst4(+) gene is a SIR2 homologue with silencing and centromeric functions.
Freeman-Cook LL et al. Mol Biol Cell 1999 Oct;10(10):3171-86
PMID:23771057 - Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.
Alper BJ et al. EMBO J 2013 Aug 28;32(17):2321-35
PMID:24687850 - H2A.Z-dependent regulation of cohesin dynamics on chromosome arms.
Tapia-Alveal C et al. Mol Cell Biol 2014 Jun;34(11):2092-104
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26890608 - A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
Gaspa L et al. Cell Cycle 2016;15(5):720-9