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protein coding gene - bsu1 (SPAC17A2.01) - plasma membrane high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1

Gene summary

Standard name
bsu1
Systematic ID
SPAC17A2.01
Product
plasma membrane high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
sod1, car1, SPAC1B1.05
UniProt ID
P33532
ORFeome ID
45/45C01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3550034..3552067 forward strand

Annotation

GO biological process

GO:1903090 - pyridoxal transmembrane transport

References:

GO:1903091 - pyridoxamine transmembrane transport

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GO:1903092 - pyridoxine transmembrane transport

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GO:0071934 - thiamine transmembrane transport

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GO cellular component

GO:0000324 - fungal-type vacuole

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GO:0005886 - plasma membrane

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GO molecular function

GO:0031925 - pyridoxal transmembrane transporter activity

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GO:0031927 - pyridoxamine transmembrane transporter activity

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GO:0031928 - pyridoxine transmembrane transporter activity

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GO:0015234 - thiamine transmembrane transporter activity

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000022 - protein absent

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PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000021 - protein present

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PomGeneEx:0000015 - RNA absent

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0003366 - decreased pyridoxine import

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Genotypes:

FYPO:0000636 - increased cell population growth rate

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0002805 - normal sodium import

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000125 - resistance to amiloride

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0000110 - orthologs cannot be distinguished

Warnings

PBO:0001338 - gene name conflict

PBO:0002112 - S. cerevisiae PN uptake is mediated by the unrelated transporter Tpn1p

Protein features

IDNameInterPro nameDB name
PF07690MFS_1MFSPfam
cd17323MFS_Tpo1_MDR_likeCDD
PS50850MFSMFS_domPROSITE profiles
G3DSA:1.20.1250.20:FF:000596CATH-FunFam
G3DSA:1.20.1250.20MFS_trans_sfCATH-Gene3D
SSF103473MFS_trans_sfSUPERFAMILY
PTHR23502PANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:15701794 - Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+).
Stolz J et al. Eukaryot Cell 2005 Feb;4(2):319-26
PMID:18201975 - Characterization of Thi9, a novel thiamine (Vitamin B1) transporter from Schizosaccharomyces pombe.
Vogl C et al. J Biol Chem 2008 Mar 21;283(12):7379-89
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21832151 - Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis.
Kashiwazaki J et al. Mol Biol Cell 2011 Oct;22(19):3658-70
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25117315 - A potential protective role for thiamine in glucose-driven oxidative stress.
Palabiyik B et al. Genet Mol Res 2014 Jul 25;13(3):5582-93
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25102102 - A metabolic strategy to enhance long-term survival by Phx1 through stationary phase-specific pyruvate decarboxylases in fission yeast.
Kim JY et al. Aging (Albany NY) 2014 Jul;6(7):587-601
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:8246883 - The amiloride resistance gene, car1, of Schizosaccharomyces pombe.
Jia ZP et al. Mol Gen Genet 1993 Nov;241(3-4):298-304
PMID:8675019 - Amiloride toxicity in the fission yeast Schizosaccharomyces pombe is released by thiamine and mutations in the thiamine-repressible gene car1.
Niederberger C et al. Gene 1996 May 24;171(1):119-22
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399