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protein coding gene - ptr8 (SPAC17A5.06) - transcription factor TFIIH complex DNA helicase (human ERCC3) subunit Ptr8

Gene summary

Standard name
ptr8
Systematic ID
SPAC17A5.06
Product
transcription factor TFIIH complex DNA helicase (human ERCC3) subunit Ptr8
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ercc3
UniProt ID
O13768
ORFeome ID
28/28F10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1761547..1764319 forward strand

Annotation

Disease association

MONDO:0002470 - photosensitive trichothiodystrophy

References:

MONDO:0014615 - trichothiodystrophy 2, photosensitive

References:

MONDO:0012531 - xeroderma pigmentosum group B

References:

GO biological process

GO:0006367 - transcription initiation at RNA polymerase II promoter

References:

GO:0006283 - transcription-coupled nucleotide-excision repair

References:

GO cellular component

GO:0000112 - nucleotide-excision repair factor 3 complex

References:

GO:0005634 - nucleus

References:

GO:0000439 - transcription factor TFIIH core complex

References:

GO:0005675 - transcription factor TFIIH holo complex

References:

GO:0097550 - transcription preinitiation complex

References:

GO molecular function

GO:0043138 - 3'-5' DNA helicase activity

References:

GO:0005524 - ATP binding

References:

GO:0003677 - DNA binding

References:

GO:0016787 - hydrolase activity

References:

GO:0016251 - RNA polymerase II general transcription initiation factor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04851ResIIIHelicase/UvrB_NPfam
PF13625Helicase_C_3XPB/Ssl2_NPfam
PF16203ERCC3_RAD25_CERCC3_RAD25_CPfam
cd18029DEXHc_XPBCDD
cd18789SF2_C_XPBERCC3_RAD25_CCDD
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE profiles
PS51194HELICASE_CTERHelicase_C-likePROSITE profiles
SM00487DEXDcHelicase_ATP-bdSMART
SM00490HELICcHelicase_C-likeSMART
PR00851XRODRMPGMNTBPRINTS
G3DSA:3.40.50.300:FF:000077CATH-FunFam
G3DSA:3.40.50.300:FF:000117CATH-FunFam
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR11274ATP-dep_DNA_HelicasePANTHER
TIGR00603rad25XPB/Ssl2NCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:15161942 - The nucleolus is involved in mRNA export from the nucleus in fission yeast.
Ideue T et al. J Cell Sci 2004 Jun 15;117(Pt 14):2887-95
PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.
Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PB_REF:0000003 - Disease Association Curation
PMID:14534314 - Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro.
Spahr H et al. J Biol Chem 2003 Dec 19;278(51):51301-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:17212653 - Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast.
Mizuki F et al. Genes Cells 2007 Jan;12(1):35-47
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:8303296 - RNA polymerase II initiation factor interactions and transcription start site selection.
Li Y et al. Science 1994 Feb 11;263(5148):805-7
PMID:15937491 - TFIIH XPB mutants suggest a unified bacterial-like mechanism for promoter opening but not escape.
Lin YC et al. Nat Struct Mol Biol 2005 Jul;12(7):603-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000002 - Comments
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.