protein coding gene - nuc2 (SPAC17C9.01c) - anaphase-promoting complex TPR lobe subcomplex subunit Apc3

Gene summary

Standard name

nuc2

Systematic ID

SPAC17C9.01c

Product

anaphase-promoting complex TPR lobe subcomplex subunit Apc3

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

apc3, SPAC1851.01

UniProt ID

P10505

ORFeome ID

33/33F10

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 4498096..4500581 forward strand

Annotation

Protein features

Orthologs

• CDC27 (HGNC:1728) Homo sapiens
• nuc2 (SJAG_01956) Schizosaccharomyces japonicus
• CDC27 (YBL084C) Saccharomyces cerevisiae

References / Literature

PMID:10526233 - Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses.

Yamashita YM et al. Genes Cells 1999 Aug;4(8):445-63

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55

PMID:12477395 - Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes.

Yoon HJ et al. Curr Biol 2002 Dec 10;12(23):2048-54

PMID:11950879 - Control of localization of a spindle checkpoint protein, Mad2, in fission yeast.

Ikui AE et al. J Cell Sci 2002 Apr 15;115(Pt 8):1603-10

PMID:8918880 - 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway.

Yamashita YM et al. Nature 1996 Nov 21;384(6606):276-9

PMID:7622618 - Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation.

Kumada K et al. J Cell Sci 1995 Mar;108 ( Pt 3):895-905

PMID:18082611 - Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.

Ohi MD et al. Mol Cell 2007 Dec 14;28(5):871-85

PMID:18331722 - A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast.

Kimata Y et al. Dev Cell 2008 Mar;14(3):446-54

PMID:19117951 - Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast.

Dubey RN et al. J Biol Chem 2009 Mar 13;284(11):7165-76

PMID:16950791 - Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function.

Yoon HJ et al. J Biol Chem 2006 Oct 27;281(43):32284-93

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.

Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266

PMID:3283148 - A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase.

Hirano T et al. J Cell Biol 1988 Apr;106(4):1171-83

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:7798319 - Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase.

Samejima I et al. J Cell Biol 1994 Dec;127(6 Pt 1):1655-70

PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.

Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397

PMID:2297790 - Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+.

Hirano T et al. Cell 1990 Jan 26;60(2):319-28

PMID:2404993 - In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.

Masuda H et al. J Cell Biol 1990 Feb;110(2):417-25

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:11033806 - [Overexpression of the apc10+ gene in the fission yeast Schizosaccharomyces pombe can suppress temperature sensitivity of the nuc2-663 mutant,but not its sterility].

Grishchuk EL et al. Mol Biol (Mosk) 2000;34(5):809-15

PMID:3409871 - Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis.

Ohkura H et al. EMBO J 1988 May;7(5):1465-73

PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).

Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47

PMID:33506191 - Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.

Yukawa M et al. iScience 2021 Jan 22;24(1):102031

PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.

Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99

PMID:8978689 - Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+.

Ishii K et al. EMBO J 1996 Dec 02;15(23):6629-40

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:18225957 - Nuc2p, a subunit of the anaphase-promoting complex, inhibits septation initiation network following cytokinesis in fission yeast.

Chew TG et al. PLoS Genet 2008 Jan;4(1):e17

PMID:9383050 - Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein.

Forsburg SL et al. Genetics 1997 Nov;147(3):1025-41

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:8388878 - Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.

Funabiki H et al. J Cell Biol 1993 Jun;121(5):961-76

PMID:15060174 - Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27.

Schwickart M et al. Mol Cell Biol 2004 Apr;24(8):3562-76

PMID:9614176 - Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast.

Stern B et al. Mol Biol Cell 1998 Jun;9(6):1309-21

PMID:15161942 - The nucleolus is involved in mRNA export from the nucleus in fission yeast.

Ideue T et al. J Cell Sci 2004 Jun 15;117(Pt 14):2887-95

PMID:9649518 - A screen for genes involved in the anaphase proteolytic pathway identifies tsm1(+), a novel Schizosaccharomyces pombe gene important for microtubule integrity.

Grishchuk EL et al. Genetics 1998 Jul;149(3):1251-64

PMID:9755167 - The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.

Yamano H et al. EMBO J 1998 Oct 01;17(19):5670-8

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:9264466 - Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast.

Yamada H et al. J Cell Sci 1997 Aug;110 ( Pt 15):1793-804

PMID:9736616 - Apc10 and Ste9/Srw1, two regulators of the APC-cyclosome, as well as the CDK inhibitor Rum1 are required for G1 cell-cycle arrest in fission yeast.

Kominami K et al. EMBO J 1998 Sep 15;17(18):5388-99

PMID:9755169 - Cdc18 transcription and proteolysis couple S phase to passage through mitosis.

Baum B et al. EMBO J 1998 Oct 01;17(19):5689-98

PMID:10725227 - A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.

Grallert B et al. J Cell Sci 2000 Apr;113 ( Pt 8):1447-58

PMID:10082519 - The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function.

Berry LD et al. Mol Cell Biol 1999 Apr;19(4):2535-46

PMID:9223296 - The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex.

He X et al. Proc Natl Acad Sci U S A 1997 Jul 22;94(15):7965-70

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:2561424 - Gene products required for chromosome separation.

Yanagida M J Cell Sci Suppl 1989;12:213-29

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:18354085 - Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.

Kimata Y et al. J Cell Sci 2008 Apr 01;121(Pt 7):1107-18

PMID:11533255 - Study of cyclin proteolysis in anaphase-promoting complex (APC) mutant cells reveals the requirement for APC function in the final steps of the fission yeast septation initiation network.

Chang L et al. Mol Cell Biol 2001 Oct;21(19):6681-94

PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053