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protein coding gene - lys7 (SPAC17C9.02c) - alpha-aminoadipate reductase phosphopantetheinyl transferase Lys7

Gene summary

Standard name
lys7
Systematic ID
SPAC17C9.02c
Product
alpha-aminoadipate reductase phosphopantetheinyl transferase Lys7
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10474
ORFeome ID
10/10E11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4496725..4498140 forward strand

Annotation

Experimental tools

PBO:0003176 - selectable marker, amino acid auxotroph

References:

GO biological process

GO:0009085 - L-lysine biosynthetic process

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GO cellular component

GO:0005829 - cytosol

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GO molecular function

GO:0008897 - holo-[acyl-carrier-protein] synthase activity

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GO:0000287 - magnesium ion binding

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Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004908 - abolished L-aminoadipate-semialdehyde dehydrogenase activity

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0002725 - decreased L-aminoadipate-semialdehyde dehydrogenase activity

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000039 - growth auxotrophic for lysine

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0003824 - resistance to caffeine and rapamycin

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Genotypes:

FYPO:0002634 - resistance to cobalt

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01648ACPS4-PPantetheinyl_Trfase_domPfam
PF22624AASDHPPT_NAASDHPPT_NPfam
G3DSA:3.90.470.204-PPantetheinyl_Trfase_dom_sfCATH-Gene3D
SSF562144-PPantetheinyl_Trfase_dom_sfSUPERFAMILY
PTHR12215P-Pant_transferase_sfPANTHER

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:3142867 - Lysine biosynthesis pathway and biochemical blocks of lysine auxotrophs of Schizosaccharomyces pombe.
Ye ZH et al. J Bacteriol 1988 Dec;170(12):5968-70
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22198627 - Use of a ura5+-lys7+ cassette to construct unmarked gene knock-ins in Schizosaccharomyces pombe.
Mudge DK et al. Curr Genet 2012 Feb;58(1):59-64
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
PMID:12127488 - Functional characterization of 4'-phosphopantetheinyl transferase genes of bacterial and fungal origin by complementation of Saccharomyces cerevisiae lys5.
Mootz HD et al. FEMS Microbiol Lett 2002 Jul 16;213(1):51-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:17614284 - The chromatin-remodeling factor FACT contributes to centromeric heterochromatin independently of RNAi.
Lejeune E et al. Curr Biol 2007 Jul 17;17(14):1219-24
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
GO_REF:0000002 - Comments
PMID:15546125 - Posttranslational activation, site-directed mutation and phylogenetic analyses of the lysine biosynthesis enzymes alpha-aminoadipate reductase Lys1p (AAR) and the phosphopantetheinyl transferase Lys7p (PPTase) from Schizosaccharomyces pombe.
Guo S et al. Yeast 2004 Nov;21(15):1279-88
PMID:32435206 - Posttranslational Arginylation Enzyme Arginyltransferase1 Shows Genetic Interactions With Specific Cellular Pathways in vivo .
Wiley DJ et al. Front Physiol 2020;11:427
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025