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protein coding gene - pnu1 (SPAC17C9.08) - mitochondrial endodeoxyribonuclease Pnu1

Gene summary

Standard name
pnu1
Systematic ID
SPAC17C9.08
Product
mitochondrial endodeoxyribonuclease Pnu1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
nuc1, end1
UniProt ID
Q10480
ORFeome ID
44/44A10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4484695..4486032 reverse strand

Annotation

GO cellular component

GO:0005743 - mitochondrial inner membrane

References:

GO:0005739 - mitochondrion

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GO:0005634 - nucleus

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GO molecular function

GO:0004520 - DNA endonuclease activity

References:

GO:0004536 - DNA nuclease activity

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GO:0046872 - metal ion binding

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GO:0004521 - RNA endonuclease activity

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GO:0004540 - RNA nuclease activity

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GO:0000014 - single-stranded DNA endonuclease activity

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Modification

MOD:00006 - N-glycosylated residue

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Multi-locus phenotype

FYPO:0002035 - decreased DNA topoisomerase type I activity

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Genotypes:

FYPO:0002037 - decreased endodeoxyribonuclease activity

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

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Genotypes:

FYPO:0000590 - normal sporulation

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003164 - abolished nuclease activity

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0002037 - decreased endodeoxyribonuclease activity

References:

Genotypes:

FYPO:0005891 - decreased nuclease activity during nitrogen starvation

References:

Genotypes:

FYPO:0005295 - decreased RNA catabolic process during nitrogen starvation

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Genotypes:

FYPO:0000160 - fragmented DNA

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004016 - normal cellular mtDNA level

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

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Genotypes:

FYPO:0000488 - normal meiotic recombination

References:

Genotypes:

FYPO:0001096 - normal RNA level during nitrogen starvation

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Genotypes:

FYPO:0000943 - normal spore morphology

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Genotypes:

FYPO:0000590 - normal sporulation

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF01223Endonuclease_NSEndo_G_ENPP1-like_domPfam
cd00091NUCCDD
PS01070NUCLEASE_NON_SPECDNA/RNA_endonuclease_ASPROSITE patterns
SM00477NUCENPP1-3/EXOG-like_nuc-likeSMART
SM00892Endonuclease_NSEndo_G_ENPP1-like_domSMART
G3DSA:3.40.570.10:FF:000004CATH-FunFam
G3DSA:3.40.570.10DNA/RNA_non-sp_Endonuclease_sfCATH-Gene3D
SSF54060His-Me_finger_sfSUPERFAMILY
PTHR13966Non-specific_endonucleasePANTHER
Signal PeptideSignal-Peptide-DeepTMHMM

Orthologs

References / Literature

PMID:11872168 - Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation.
Nakashima A et al. J Biochem 2002 Mar;131(3):391-8
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:6090122 - Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.
Uemura T et al. EMBO J 1984 Aug;3(8):1737-44
GO_REF:0000002 - Comments
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11406279 - Isolation and characterization of the Schizosaccharomyces pombe cDNA encoding the mitochondrial endonuclease(1).
Ikeda S et al. Biochim Biophys Acta 2001 May 28;1519(1-2):111-6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:18488015 - Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution.
Wilhelm BT et al. Nature 2008 Jun 26;453(7199):1239-43
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:17192844 - The end1 gene of Schizosaccharomyces pombe coding for a DNase is identical with the pnu1 gene coding for an RNase.
Sakem B et al. Yeast 2007 Jan;24(1):11-6
PMID:18047809 - Ectopic expression of mitochondria endonuclease Pnu1p from Schizosaccharomyces pombe induces cell death of the yeast.
Oda K et al. J Biochem Mol Biol 2007 Nov 30;40(6):1095-9
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623