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protein coding gene - scs22 (SPAC17C9.12) - VAP family protein Scs22

Gene summary

Standard name
scs22
Systematic ID
SPAC17C9.12
Product
VAP family protein Scs22
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10484
ORFeome ID
20/20D01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4477935..4479951 reverse strand

Annotation

Disease association

MONDO:0008453 - adult-onset proximal spinal muscular atrophy, autosomal dominant

References:

MONDO:0012077 - amyotrophic lateral sclerosis type 8

References:

MONDO:0005071 - nervous system disorder

References:

GO biological process

GO:0090158 - endoplasmic reticulum membrane organization

References:

GO:0007029 - endoplasmic reticulum organization

References:

GO:0051685 - maintenance of ER location

References:

GO:1902647 - negative regulation of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process

References:

GO:0007009 - plasma membrane organization

References:

GO:0061709 - reticulophagy

References:

GO cellular component

GO:0032541 - cortical endoplasmic reticulum

References:

GO:0160219 - cortical endoplasmic reticulum membrane

References:

GO:0005829 - cytosol

References:

GO:0005789 - endoplasmic reticulum membrane

References:

GO:0016328 - lateral plasma membrane

References:

GO:0005635 - nuclear envelope

References:

GO:0032153 - cell division site

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GO molecular function

GO:0160214 - endoplasmic reticulum-plasma membrane adaptor activity

References:

GO:0035091 - phosphatidylinositol binding

References:

GO:0001786 - phosphatidylserine binding

References:

GO:0005515 - protein binding

References:

GO:0043495 - protein-membrane adaptor activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000230 - abnormal actomyosin contractile ring actin filament organization

References:

Genotypes:

FYPO:0000161 - abnormal actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0002872 - abnormal endoplasmic reticulum localization

References:

Genotypes:

FYPO:0003444 - abnormal medial cortical node condensation

References:

Genotypes:

FYPO:0001401 - abnormal protein localization to cell division site

References:

Genotypes:

FYPO:0001009 - abolished actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0007449 - abolished protein localization to endoplasmic reticulum, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0002128 - abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0007447 - abolished reticulophagy during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0000134 - branched, elongated, multiseptate cell

References:

Genotypes:

FYPO:0006330 - decreased endoplasmic reticulum-plasma membrane tethering

References:

Genotypes:

FYPO:0000419 - decreased rate of cytokinesis

References:

Genotypes:

FYPO:0004085 - decreased vegetative cell growth

References:

Genotypes:

FYPO:0007263 - eisosomes present in decreased numbers

References:

Genotypes:

FYPO:0005840 - incomplete, asymmetric septum

References:

Genotypes:

FYPO:0006626 - increased phosphatidylinositol-4,5-bisphosphate level in plasma membrane

References:

Genotypes:

FYPO:0008330 - increased phosphatidylinositol-4-phosphate level in plasma membrane

References:

Genotypes:

FYPO:0003337 - increased protein localization to septum

References:

Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0006186 - increased rate of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

References:

Genotypes:

FYPO:0006629 - normal phosphatidylinositol-4-phosphate level in plasma membrane

References:

Genotypes:

FYPO:0007441 - normal protein localization to endoplasmic reticulum-plasma membrane contact site

References:

Genotypes:

FYPO:0002558 - normal protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

References:

Genotypes:

FYPO:0001945 - normal protein secretion

References:

Genotypes:

FYPO:0002873 - normal septum orientation

References:

Genotypes:

FYPO:0002874 - premature protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0004730 - protein mislocalized to lateral cell cortex

References:

Genotypes:

FYPO:0005264 - resistance to dithiothreitol

References:

Genotypes:

FYPO:0000843 - sensitive to dithiothreitol

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002872 - abnormal endoplasmic reticulum localization

References:

Genotypes:

FYPO:0007449 - abolished protein localization to endoplasmic reticulum, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0006330 - decreased endoplasmic reticulum-plasma membrane tethering

References:

Genotypes:

FYPO:0008328 - decreased phosphatidylinositol binding

References:

Genotypes:

FYPO:0008077 - decreased phosphatidylserine binding

References:

Genotypes:

FYPO:0007444 - normal macroautophagy during cellular response to endoplasmic reticulum stress

References:

Genotypes:

FYPO:0006294 - normal macroautophagy during nitrogen starvation

References:

Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

References:

Genotypes:

FYPO:0000843 - sensitive to dithiothreitol

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00635Motile_SpermMSP_domPfam
PS50202MSPMSP_domPROSITE profiles
G3DSA:2.60.40.10:FF:000813CATH-FunFam
G3DSA:2.60.40.10Ig-like_foldCATH-Gene3D
SSF49354PapD-like_sfSUPERFAMILY
PTHR10809VAPPANTHER
PIRSF019693VAMP-associatedVAPPIRSF
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:29290560 - ER-PM Contacts Restrict Exocytic Sites for Polarized Morphogenesis.
Ng AYE et al. Curr Biol 2018 Jan 08;28(1):146-153.e5
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26877082 - ER-PM Contacts Define Actomyosin Kinetics for Proper Contractile Ring Assembly.
Zhang D et al. Curr Biol 2016 Mar 07;26(5):647-53
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23041194 - Plasma membrane tethering of the cortical ER necessitates its finely reticulated architecture.
Zhang D et al. Curr Biol 2012 Nov 06;22(21):2048-52
PMID:32735772 - A UPR-Induced Soluble ER-Phagy Receptor Acts with VAPs to Confer ER Stress Resistance.
Zhao D et al. Mol Cell 2020 Sep 17;79(6):963-977.e3
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:35609605 - Coordinated cortical ER remodeling facilitates actomyosin ring assembly.
Zhang D et al. Curr Biol 2022 Jun 20;32(12):2694-2703.e4
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37939137 - The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast.
Zou CX et al. PLoS Biol 2023 Nov;21(11):e3002372
PMID:40629316 - Ca 2+ -dependent vesicular and non-vesicular lipid transfer controls hypoosmotic plasma membrane expansion.
Mu B et al. BMC Biol 2025 Jul 09;23(1):207
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39110593 - VAP-mediated membrane-tethering mechanisms implicate ER-PM contact function in pH homeostasis.
Hoh KL et al. Cell Rep 2024 Aug 05;43(8):114592
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:32023460 - Plasma Membrane Furrows Control Plasticity of ER-PM Contacts.
Ng AQE et al. Cell Rep 2020 Feb 04;30(5):1434-1446.e7
PMID:39239853 - Fission yeast Duc1 links to ER-PM contact sites and influences PM lipid composition and cytokinetic ring anchoring.
Willet AH et al. J Cell Sci 2024 Sep 06;
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:32320462 - Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
Marek M et al. J Cell Biol 2020 Jun 01;219(6)
PMID:15372378 - A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis.
Nishimura AL et al. Am J Hum Genet 2004 Nov;75(5):822-31