protein coding gene - cdc45 (SPAC17D4.02) - DNA replication pre-initiation complex subunit Cdc45

Gene summary

Standard name

cdc45

Systematic ID

SPAC17D4.02

Product

DNA replication pre-initiation complex subunit Cdc45

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

sna41, goa1

UniProt ID

O74113

ORFeome ID

39/39F03

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 4724772..4726846 forward strand

Annotation

Protein features

Orthologs

• CDC45 (HGNC:1739) Homo sapiens
• cdc45 (SJAG_00327) Schizosaccharomyces japonicus
• CDC45 (YLR103C) Saccharomyces cerevisiae

References / Literature

PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.

Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34

PMID:41525994 - Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.

Xu R et al. Nucleic Acids Res 2026 Jan 05;54(1)

PMID:22024164 - Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.

Matsumoto S et al. J Cell Biol 2011 Oct 31;195(3):387-401

PMID:34108240 - The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes.

Liu Y et al. Proc Natl Acad Sci U S A 2021 Jun 15;118(24)

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:31719112 - Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.

Kim SM et al. G3 (Bethesda) 2020 Jan 07;10(1):255-266

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:9619628 - Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe.

Miyake S et al. Genes Cells 1998 Mar;3(3):157-66

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:21099360 - Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.

Matsumoto S et al. Cell Cycle 2010 Dec 01;9(23):4627-37

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:15338237 - Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.

Dolan WP et al. Chromosoma 2004 Sep;113(3):145-56

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:11523776 - Sna41goa1, a novel mutation causing G1/S arrest in fission yeast, is defective in a CDC45 homolog and interacts genetically with polalpha.

Uchiyama M et al. Mol Genet Genomics 2001 Aug;265(6):1039-49

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:12006645 - SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.

Nakajima R et al. Mol Biol Cell 2002 May;13(5):1462-72

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.

Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684

PMID:16990792 - Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.

Yabuuchi H et al. EMBO J 2006 Oct 04;25(19):4663-74

PMID:17112379 - The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.

Locovei AM et al. Cell Div 2006 Nov 17;1:27

PMID:22279046 - Rif1 is a global regulator of timing of replication origin firing in fission yeast.

Hayano M et al. Genes Dev 2012 Jan 15;26(2):137-50

PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.

Kanke M et al. EMBO J 2012 May 02;31(9):2182-94

PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:11344166 - Essential role of Sna41/Cdc45 in loading of DNA polymerase alpha onto minichromosome maintenance proteins in fission yeast.

Uchiyama M et al. J Biol Chem 2001 Jul 13;276(28):26189-96

PMID:12972571 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.

Gregan J et al. Mol Biol Cell 2003 Sep;14(9):3876-87

PMID:15194812 - A novel intermediate in initiation complex assembly for fission yeast DNA replication.

Yamada Y et al. Mol Biol Cell 2004 Aug;15(8):3740-50

PMID:21518960 - Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.

Hayano M et al. Mol Cell Biol 2011 Jun;31(12):2380-91

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.

Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:15388803 - Identification and cloning of two putative subunits of DNA polymerase epsilon in fission yeast.

Spiga MG et al. Nucleic Acids Res 2004;32(16):4945-53

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:16849602 - Regulation of DNA replication machinery by Mrc1 in fission yeast.

Nitani N et al. Genetics 2006 Sep;174(1):155-65

PMID:19109429 - GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.

Pai CC et al. Mol Biol Cell 2009 Feb;20(4):1213-22

PMID:31262821 - Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks.

Feng G et al. Proc Natl Acad Sci U S A 2019 Jul 16;116(29):14563-14572

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53