PomBase home

protein coding gene - cis4 (SPAC17D4.03c) - Golgi zinc importer, CDF family, Cis4

Gene summary

Standard name
cis4
Systematic ID
SPAC17D4.03c
Product
Golgi zinc importer, CDF family, Cis4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9HGQ3
ORFeome ID
33/33F11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4726782..4729341 reverse strand

Annotation

Disease association

MONDO:0957595 - Ziegler-Huang syndrome

References:

GO biological process

GO:0006882 - intracellular zinc ion homeostasis

References:

GO:1904257 - zinc ion import into Golgi lumen

References:

GO:0062111 - zinc ion import into organelle

References:

GO cellular component

GO:0031410 - cytoplasmic vesicle

References:

GO:1990674 - Golgi cis cisterna membrane

References:

GO:0000139 - Golgi membrane

References:

GO molecular function

GO:0005385 - zinc ion transmembrane transporter activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

Multi-locus phenotype

FYPO:0007042 - decreased alkaline phosphatase activity

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0007041 - increased alkaline phosphatase activity

References:

Genotypes:

FYPO:0006828 - increased cytosolic zinc level

References:

Genotypes:

FYPO:0007047 - normal alkaline phosphatase activity

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000357 - abnormal shmoo morphology

References:

Genotypes:

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0006010 - abolished shmoo contact with partner cell

References:

Genotypes:

FYPO:0006334 - decreased RNA level during cellular response to zinc ion starvation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0006828 - increased cytosolic zinc level

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0007048 - normal alkaline phosphatase activity during stress response to zinc ion

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF01545Cation_effluxCation_efflux_TMPFAM
SSF161111Cation efflux protein transmembrane domain-likeCation_efflux_TMD_sfSUPERFAMILY
G3DSA:1.20.1510.10Cation efflux protein transmembrane domainCation_efflux_TMD_sfGENE3D
PTHR45755Msc2-likePANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
TIGR01297CDFCation_effluxNCBIFAM

Orthologs

References / Literature

PMID:18199682 - Cation diffusion facilitator Cis4 is implicated in Golgi membrane trafficking via regulating zinc homeostasis in fission yeast.
Fang Y et al. Mol Biol Cell 2008 Apr;19(4):1295-303
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22848669 - Studies on the roles of clathrin-mediated membrane trafficking and zinc transporter Cis4 in the transport of GPI-anchored proteins in fission yeast.
Jaiseng W et al. PLoS One 2012;7(7):e41946
PMID:29529046 - Zinc transporters belonging to the Cation Diffusion Facilitator (CDF) family have complementary roles in transporting zinc out of the cytosol.
Choi S et al. PLoS Genet 2018 Mar;14(3):e1007262
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:31239353 - Zinc-dependent activation of the Pho8 alkaline phosphatase in Schizosaccharomyces pombe .
Hu YM et al. J Biol Chem 2019 Aug 16;294(33):12392-12404
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:24454826 - E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.
Fang Y et al. PLoS One 2014;9(1):e85238
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6