PomBase home

protein coding gene - pof8 (SPAC17G6.17) - LARP7 family RNA-binding protein Pof8/Lar7

Gene summary

Standard name
pof8
Systematic ID
SPAC17G6.17
Product
LARP7 family RNA-binding protein Pof8/Lar7
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
lar7
UniProt ID
O13795
ORFeome ID
44/44A06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3623723..3625371 forward strand

Annotation

Disease association

MONDO:0014031 - microcephalic primordial dwarfism, Alazami type

References:

GO biological process

GO:0071027 - nuclear RNA surveillance

References:

GO:0016180 - snRNA processing

References:

GO:1904868 - telomerase catalytic core complex assembly

References:

GO:0090669 - telomerase RNA stabilization

References:

GO:0007004 - telomere maintenance via telomerase

References:

GO cellular component

GO:0140445 - chromosome, telomeric repeat region

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0005732 - sno(s)RNA-containing ribonucleoprotein complex

References:

GO:0005697 - telomerase holoenzyme complex

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0070034 - telomerase RNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

References:

Genotypes:

FYPO:0005540 - decreased telomerase RNA reverse transcriptase activity

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0001890 - increased RNA level

References:

Genotypes:

FYPO:0002357 - normal protein-RNA interaction

References:

Genotypes:

FYPO:0000840 - normal RNA level

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0003106 - stable shortened telomeres during vegetative growth

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0007394 - abnormal RNA structure

References:

Genotypes:

FYPO:0008112 - abnormal U6 snRNP assembly

References:

Genotypes:

FYPO:0007884 - abolished Lsm2-8 complex binding

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0002133 - abolished protein-RNA interaction

References:

Genotypes:

FYPO:0007885 - decreased Lsm2-8 complex binding

References:

Genotypes:

FYPO:0006466 - decreased mature ncRNA level

References:

Genotypes:

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

References:

Genotypes:

FYPO:0000826 - decreased RNA level

References:

Genotypes:

FYPO:0005540 - decreased telomerase RNA reverse transcriptase activity

References:

Genotypes:

FYPO:0006848 - decreased total RNA level during vegetative growth

References:

Genotypes:

FYPO:0008110 - decreased U6 2'-O-snRNA methylation at residue A64

References:

Genotypes:

FYPO:0004656 - increased protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0007845 - increased rate of RNA catabolic process during vegetative growth

References:

Genotypes:

FYPO:0002908 - increased transcription at telomere during vegetative growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003555 - normal chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0000478 - normal meiosis

References:

Genotypes:

FYPO:0004083 - normal protein level

References:

Genotypes:

FYPO:0002887 - normal protein localization to telomere during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0002357 - normal protein-RNA interaction

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0005538 - normal telomere-telomerase complex assembly

References:

Genotypes:

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0003106 - stable shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF19977xRRMLar7_xRRMPFAM
cd21611RRM_SpPof8_likeCDD
PS51939XRRMLa_xRRMPROSITE_PROFILES
G3DSA:3.30.70.330Nucleotide-bd_a/b_plait_sfGENE3D

Orthologs

References / Literature

PMID:33131423 - Structure of S. pombe telomerase protein Pof8 C-terminal domain is an xRRM conserved among LARP7 proteins.
Basu R et al. RNA Biol 2021 Aug;18(8):1181-1192
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:15147268 - Molecular interactions of fission yeast Skp1 and its role in the DNA damage checkpoint.
Lehmann A et al. Genes Cells 2004 May;9(5):367-82
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
InterPro:IPR014886 - Unknown title
PMID:29422503 - LARP7-like protein Pof8 regulates telomerase assembly and poly(A)+TERRA expression in fission yeast.
Mennie AK et al. Nat Commun 2018 Feb 08;9(1):586
PMID:29422664 - Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast.
Páez-Moscoso DJ et al. Nat Commun 2018 Feb 08;9(1):587
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:33378677 - Quality-Control Mechanism for Telomerase RNA Folding in the Cell.
Hu X et al. Cell Rep 2020 Dec 29;33(13):108568
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:35277511 - The methyl phosphate capping enzyme Bmc1/Bin3 is a stable component of the fission yeast telomerase holoenzyme.
Porat J et al. Nat Commun 2022 Mar 11;13(1):1277
PMID:37403782 - The fission yeast methyl phosphate capping enzyme Bmc1 guides 2'-O-methylation of the U6 snRNA.
Porat J et al. Nucleic Acids Res 2023 Sep 08;51(16):8805-8819
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19748355 - F-box-directed CRL complex assembly and regulation by the CSN and CAND1.
Schmidt MW et al. Mol Cell 2009 Sep 11;35(5):586-97
PMID:29422501 - LARP7 family proteins have conserved function in telomerase assembly.
Collopy LC et al. Nat Commun 2018 Feb 08;9(1):557