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protein coding gene - sec3 (SPAC17G8.12) - exocyst complex subunit Exo1

Gene summary

Standard name
sec3
Systematic ID
SPAC17G8.12
Product
exocyst complex subunit Exo1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
exo1
UniProt ID
Q10324
ORFeome ID
36/36F09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2363286..2367055 forward strand

Annotation

Complementation

PBO:0013769 - functionally complemented by human EXOC1

References:

GO biological process

GO:0006887 - exocytosis

References:

GO:0090522 - vesicle tethering involved in exocytosis

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GO cellular component

GO:0051285 - cell cortex of cell tip

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GO:1902716 - cell cortex of growing cell tip

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GO:0032153 - cell division site

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GO:0051286 - cell tip

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GO:0005829 - cytosol

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GO:0000935 - division septum

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GO:0000145 - exocyst

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GO molecular function

GO:0005546 - phosphatidylinositol-4,5-bisphosphate binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001585 - abolished protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0001045 - decreased acid phosphatase activity

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Genotypes:

FYPO:0001235 - decreased extent of cell population growth

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Genotypes:

FYPO:0002086 - exocytic vesicles present in increased numbers

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Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0001252 - multinucleate multiseptate vegetative cell

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0001903 - normal septation index

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Genotypes:

FYPO:0000673 - normal septum assembly

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Genotypes:

FYPO:0000647 - vegetative cell lysis

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002435 - abnormal actin cables

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Genotypes:

FYPO:0000230 - abnormal actomyosin contractile ring actin filament organization

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Genotypes:

FYPO:0001370 - abnormal protein localization

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Genotypes:

FYPO:0001401 - abnormal protein localization to cell division site

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Genotypes:

FYPO:0001584 - abnormal protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0001045 - decreased acid phosphatase activity

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Genotypes:

FYPO:0000422 - decreased endocytosis during vegetative growth

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Genotypes:

FYPO:0004572 - decreased exocytosis during vegetative growth

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Genotypes:

FYPO:0000835 - decreased protein level

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Genotypes:

FYPO:0006221 - decreased protein localization to cell division site, with protein mislocalized to cytoplasm

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Genotypes:

FYPO:0003208 - decreased protein localization to cell tip, with protein distributed in plasma membrane or cortex

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Genotypes:

FYPO:0003771 - decreased protein localization to plasma membrane of cell tip during vegetative growth

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Genotypes:

FYPO:0000538 - decreased protein secretion during vegetative growth

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Genotypes:

FYPO:0001365 - decreased rate of actomyosin contractile ring contraction

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000745 - delayed onset of actin cortical patch internalization

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Genotypes:

FYPO:0000424 - delayed onset of endocytosis during vegetative growth

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Genotypes:

FYPO:0000639 - delayed onset of septum assembly

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Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

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Genotypes:

FYPO:0002086 - exocytic vesicles present in increased numbers

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Genotypes:

FYPO:0002087 - exocytic vesicles present in increased numbers at cell tip during mitotic interphase

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Genotypes:

FYPO:0002088 - exocytic vesicles present in increased numbers at septum during septum assembly

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0001406 - increased septum thickness

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Genotypes:

FYPO:0002148 - inviable after spore germination, multiple cell divisions, abnormal morphology

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Genotypes:

FYPO:0001489 - inviable vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000224 - lemon-shaped cell

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

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Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0001903 - normal septation index

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002526 - sensitive to latrunculin B

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Genotypes:

FYPO:0002438 - short, misoriented actin cables

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0000024 - stubby vegetative cell

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Genotypes:

FYPO:0000647 - vegetative cell lysis

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Genotypes:

FYPO:0001496 - viable elongated multiseptate vegetative cell

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Genotypes:

FYPO:0001491 - viable vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0011449 - S. pombe is missing the conserved C-terminal region found in other species

References:

Protein features

IDNameInterPro nameDB name
PF15278Sec3_C_2Sec3_C_2PFAM
PF15277Sec3-PIP2_bindSec3-PIP2_bindPFAM
SM01313Sec3_PIP2_bind_2Sec3-PIP2_bindSMART
G3DSA:2.30.29.90GENE3D
PTHR16092SEC3/SYNTAXIN-RELATEDPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.
Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:40931936 - Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.
Ren L et al. J Cell Sci 2025 Sep 01;138(17)
PMID:41171630 - Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis.
Singh D et al. Elife 2025 Oct 31;13
PMID:28765280 - The exocyst subunit Sec3 is regulated by a protein quality control pathway.
Kampmeyer C et al. J Biol Chem 2017 Sep 15;292(37):15240-15253
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:22768263 - Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles.
Bendezú FO et al. PLoS One 2012;7(6):e40248
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:16169489 - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast.
Gregan J et al. Curr Biol 2005 Sep 20;15(18):1663-9
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22146723 - Systematic localization study on novel proteins encoded by meiotically up-regulated ORFs in fission yeast.
Ikebe C et al. Biosci Biotechnol Biochem 2011;75(12):2364-70
PMID:22891673 - Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton.
Jourdain I et al. Traffic 2012 Nov;13(11):1481-95
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97