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protein coding gene - mtl1 (SPAC17H9.02) - MTREC (exosome adaptor) complex RNA helicase subunit Mtl1

Gene summary

Standard name
mtl1
Systematic ID
SPAC17H9.02
Product
MTREC (exosome adaptor) complex RNA helicase subunit Mtl1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13799
ORFeome ID
30/30A02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2004706..2008108 forward strand

Annotation

GO biological process

GO:0033621 - nuclear mRNA surveillance of meiosis-specific transcripts

References:

GO:0071030 - nuclear mRNA surveillance of spliceosomal pre-mRNA splicing

References:

GO:0006364 - rRNA processing

References:

GO:1902794 - siRNA-independent facultative heterochromatin formation

References:

GO:0043144 - sno(s)RNA processing

References:

GO cellular component

GO:1990342 - heterochromatin island

References:

GO:1990477 - MTREC complex

References:

GO:0016604 - nuclear body

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0031499 - TRAMP complex

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0003723 - RNA binding

References:

GO:0003724 - RNA helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003230 - decreased histone H3-K9 methylation at heterochromatin island during vegetative growth

References:

Genotypes:

FYPO:0007280 - decreased protein localization to nuclear exosome focus

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0008148 - increased CUT RNA level

References:

Genotypes:

FYPO:0008113 - increased intron retention

References:

Genotypes:

FYPO:0002960 - increased level of DSR-containing meiotic gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0002173 - increased level of meiotic gene mRNA during vegetative growth

References:

Genotypes:

FYPO:0002931 - increased poly(A) tail length

References:

Genotypes:

FYPO:0001908 - increased pre-mRNA level

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0003237 - increased RNA splicing at cryptic splice sites

References:

Genotypes:

FYPO:0002916 - increased RNA splicing, via spliceosome

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002451 - inviable vegetative cell with abnormal cell shape

References:

Genotypes:

FYPO:0002567 - normal centromeric outer repeat transcript level

References:

Genotypes:

FYPO:0003235 - normal histone H3-K9 methylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00270DEADDEAD/DEAH_box_helicase_domPfam
PF00271Helicase_CHelicase_C-likePfam
PF08148DSHCTSki2/MTR4_CPfam
PF13234MTR4_beta-barrelBeta-barrel_MTR4Pfam
PF21408MTR4-like_stalkMTR4-like_stalkPfam
cd13154KOW_Mtr4CDD
cd18024DEXHc_Mtr4-likeCDD
cd18795SF2_C_Ski2CDD
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE profiles
PS51194HELICASE_CTERHelicase_C-likePROSITE profiles
SM00487DEXDcHelicase_ATP-bdSMART
SM00490HELICcHelicase_C-likeSMART
SM01142DSHCTSki2/MTR4_CSMART
G3DSA:1.20.1500.20:FF:000002CATH-FunFam
G3DSA:2.40.30.300:FF:000005CATH-FunFam
G3DSA:3.40.50.300:FF:000083CATH-FunFam
G3DSA:3.40.50.300:FF:000141CATH-FunFam
G3DSA:1.10.3380.30CATH-Gene3D
G3DSA:1.20.1500.20CATH-Gene3D
G3DSA:2.40.30.300CATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR12131SUPV3-likePANTHER
PIRSF005198Antiviral_helicase_SKI2FRH-likePIRSF
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:26942678 - Enhancer of Rudimentary Cooperates with Conserved RNA-Processing Factors to Promote Meiotic mRNA Decay and Facultative Heterochromatin Assembly.
Sugiyama T et al. Mol Cell 2016 Mar 03;61(5):747-759
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000002 - Comments
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:38815580 - RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.
Khanduja JS et al. Cell 2024 May 21;
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:32012158 - Meiotic gene silencing complex MTREC/NURS recruits the nuclear exosome to YTH-RNA-binding protein Mmi1.
Shichino Y et al. PLoS Genet 2020 Feb;16(2):e1008598
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74
PMID:25240800 - The RNA exosome promotes transcription termination of backtracked RNA polymerase II.
Lemay JF et al. Nat Struct Mol Biol 2014 Oct;21(10):919-26
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:34209806 - Identification of Nrl1 Domains Responsible for Interactions with RNA-Processing Factors and Regulation of Nrl1 Function by Phosphorylation.
Mikolaskova B et al. Int J Mol Sci 2021 Jun 29;22(13)
PMID:28841135 - Ubiquitination-dependent control of sexual differentiation in fission yeast.
Simonetti F et al. Elife 2017 Aug 25;6
PMID:25989903 - The fission yeast MTREC complex targets CUTs and unspliced pre-mRNAs to the nuclear exosome.
Zhou Y et al. Nat Commun 2015 May 20;6:7050
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33574613 - TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation.
Wei Y et al. Nat Cell Biol 2021 Mar;23(3):243-256
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26682798 - The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast.
Aronica L et al. Nucleic Acids Res 2016 Feb 29;44(4):1703-17
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26631744 - Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.
Chalamcharla VR et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15548-55
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:36002457 - Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex.
Foucher AE et al. Nat Commun 2022 Aug 24;13(1):4969
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.
Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31
PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe .
Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536