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protein coding gene - pub2 (SPAC1805.15c) - HECT-type ubiquitin-protein ligase E3 Pub2

Gene summary

Standard name
pub2
Systematic ID
SPAC1805.15c
Product
HECT-type ubiquitin-protein ligase E3 Pub2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTG2
ORFeome ID
50/50A09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2797871..2800406 reverse strand

Annotation

Complementation

PBO:0001737 - functionally complemented by S. cerevisiae YER125W

References:

Disease association

MONDO:0008323 - Liddle syndrome

References:

MONDO:0014966 - periventricular nodular heterotopia 7

References:

GO biological process

GO:0120113 - cytoplasm to vacuole targeting by the NVT pathway

References:

GO:0006511 - ubiquitin-dependent protein catabolic process

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GO cellular component

GO:0051285 - cell cortex of cell tip

References:

GO:0032153 - cell division site

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GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0061630 - ubiquitin protein ligase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0006274 - abolished protein localization via NVT pathway

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0002462 - inviable branched, elongated vegetative cell

References:

Genotypes:

Protein features

PBO:0111864 - C2 domain

PBO:0111873 - Nedd4/Rsp5 family

References:

PBO:0111749 - ubiquitin-protein ligase E3

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PBO:0111769 - WW domain

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0001023 - normal growth on cisplatin

References:

Genotypes:

FYPO:0006014 - promiscuous mating

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00397WWWW_domPFAM
PF00632HECTHECT_domPFAM
cd00201WWWW_domCDD
cd00078HECTcHECT_domCDD
cd08382C2_Smurf-likeCDD
PS01159WW_DOMAIN_1WW_domPROSITE_PATTERNS
PS50237HECTHECT_domPROSITE_PROFILES
PS50020WW_DOMAIN_2WW_domPROSITE_PROFILES
PS50004C2C2_domPROSITE_PROFILES
SM00456ww_5WW_domSMART
SM00119hect_3HECT_domSMART
G3DSA:3.30.2160.10:FF:000001FUNFAM
G3DSA:3.30.2410.10:FF:000037FUNFAM
G3DSA:3.90.1750.10:FF:000079FUNFAM
SSF49562C2 domain (Calcium/lipid-binding domain, CaLB)C2_domain_sfSUPERFAMILY
SSF56204Hect, E3 ligase catalytic domainHect_E3_ubiquitin_ligaseSUPERFAMILY
SSF51045WW domainWW_dom_sfSUPERFAMILY
G3DSA:3.30.2160.10Hect, E3 ligase catalytic domainGENE3D
G3DSA:3.30.2410.10Hect, E3 ligase catalytic domainGENE3D
G3DSA:2.60.40.150C2 domainC2_domain_sfGENE3D
G3DSA:3.90.1750.10Hect, E3 ligase catalytic domainsGENE3D
PTHR11254HECT DOMAIN UBIQUITIN-PROTEIN LIGASEE3_ubiq-protein_ligasePANTHER
PIRSF001569E3_ub_ligase_SMURF1E3_ub_ligase_SMURF1PIRSF

Orthologs

References / Literature

PMID:11956316 - The novel HECT-type ubiquitin-protein ligase Pub2p shares partially overlapping function with Pub1p in Schizosaccharomyces pombe.
Tamai KK et al. J Cell Sci 2002 May 01;115(Pt 9):1847-57
PMID:21247416 - Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast.
Gatti L et al. BMC Genomics 2011 Jan 19;12:44
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26365378 - ESCRTs Cooperate with a Selective Autophagy Receptor to Mediate Vacuolar Targeting of Soluble Cargos.
Liu XM et al. Mol Cell 2015 Sep 17;59(6):1035-42
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:39143218 - Structural basis for transthiolation intermediates in the ubiquitin pathway.
Kochańczyk T et al. Nature 2024 Sep;633(8028):216-223
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:22194353 - Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p.
Nakase M et al. Microbiology (Reading) 2012 Mar;158(Pt 3):659-673
PB_REF:0000003 - Disease Association Curation
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24454826 - E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.
Fang Y et al. PLoS One 2014;9(1):e85238
PMID:18418059 - The Flp1/Clp1 phosphatase cooperates with HECT-type Pub1/2 protein-ubiquitin ligases in Schizosaccharomyces pombe.
Esteban V et al. Cell Cycle 2008 May 01;7(9):1269-76
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134