protein coding gene - crm1 (SPAC1805.17) - karyopherin/importin beta family nuclear export signal receptor Crm1

Gene summary

Standard name

crm1

Systematic ID

SPAC1805.17

Product

karyopherin/importin beta family nuclear export signal receptor Crm1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

caf2, SPAC1B2.01, bar1, mts10, caf7

UniProt ID

P14068

ORFeome ID

30/30C01

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2803924..2808253 forward strand

Annotation

Protein features

Orthologs

• XPO1 (HGNC:12825) Homo sapiens
• crm1 (SJAG_01358) Schizosaccharomyces japonicus
• CRM1 (YGR218W) Saccharomyces cerevisiae

References / Literature

PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:7646493 - Characterization of a novel Schizosaccharomyces pombe multidrug resistance transporter conferring brefeldin A resistance.

Turi TG et al. Biochem Biophys Res Commun 1995 Aug 15;213(2):410-8

PMID:10430904 - Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region.

Kudo N et al. Proc Natl Acad Sci U S A 1999 Aug 03;96(16):9112-7

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23209828 - Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor.

Penney M et al. PLoS One 2012;7(11):e50796

PMID:2647765 - Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery.

Adachi Y et al. J Cell Biol 1989 Apr;108(4):1195-207

PMID:28031482 - Involvement of fission yeast Pdc2 in RNA degradation and P-body function.

Wang CY et al. RNA 2017 Apr;23(4):493-503

PMID:29900664 - Tschimganine and its derivatives extend the chronological life span of yeast via activation of the Sty1 pathway.

Hibi T et al. Genes Cells 2018 Jun 14;

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:1448080 - Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1.

Toda T et al. Mol Cell Biol 1992 Dec;12(12):5474-84

PMID:16540522 - Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.

Nishijima H et al. Mol Biol Cell 2006 Jun;17(6):2524-36

PMID:14758541 - The transcription factor Pap1/Caf3 plays a central role in the determination of caffeine resistance in Schizosaccharomyces pombe.

Benko Z et al. Mol Genet Genomics 2004 Mar;271(2):161-70

PMID:19502236 - Both Php4 function and subcellular localization are regulated by iron via a multistep mechanism involving the glutaredoxin Grx4 and the exportin Crm1.

Mercier A et al. J Biol Chem 2009 Jul 24;284(30):20249-62

PMID:24478458 - The checkpoint-dependent nuclear accumulation of Rho1p exchange factor Rgf1p is important for tolerance to chronic replication stress.

Muñoz S et al. Mol Biol Cell 2014 Apr;25(7):1137-50

PMID:10485849 - Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4.

Sutani T et al. Genes Dev 1999 Sep 01;13(17):2271-83

PMID:10329722 - A novel nuclear export signal sensitive to oxidative stress in the fission yeast transcription factor Pap1.

Kudo N et al. J Biol Chem 1999 May 21;274(21):15151-8

PMID:12896976 - Schizosaccharomyces pombe cells lacking the Ran-binding protein Hba1 show a multidrug resistance phenotype due to constitutive nuclear accumulation of Pap1.

Castillo EA et al. J Biol Chem 2003 Oct 17;278(42):40565-72

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:11318103 - Sequence of Crm1/exportin 1 mutant alleles reveals critical sites associated with multidrug resistance.

Carobbio S et al. Curr Genet 2001 Feb;39(1):2-9

PMID:12181336 - Cytoplasmic localization of Wis1 MAPKK by nuclear export signal is important for nuclear targeting of Spc1/Sty1 MAPK in fission yeast.

Nguyen AN et al. Mol Biol Cell 2002 Aug;13(8):2651-63

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:29083304 - Prolyl dihydroxylation of unassembled uS12/Rps23 regulates fungal hypoxic adaptation.

Clasen SJ et al. Elife 2017 Oct 30;6

PMID:8388878 - Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.

Funabiki H et al. J Cell Biol 1993 Jun;121(5):961-76

PMID:8569688 - Caffeine-resistance in fission yeast is caused by mutations in a single essential gene, crm1+.

Kumada K et al. Mol Gen Genet 1996 Jan 15;250(1):59-68

PMID:9368044 - Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins.

Kudo N et al. J Biol Chem 1997 Nov 21;272(47):29742-51

PMID:9894913 - Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: their involvement in multidrug resistance, UV and pH sensitivity.

Benko Z et al. Mol Gen Genet 1998 Dec;260(5):434-43

PMID:12100563 - Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues.

Castillo EA et al. Mol Microbiol 2002 Jul;45(1):243-54

PMID:17384198 - Crm1-mediated nuclear export of the Schizosaccharomyces pombe transcription factor Cuf1 during a shift from low to high copper concentrations.

Beaudoin J et al. Eukaryot Cell 2007 May;6(5):764-75

PMID:18799626 - Fission yeast kinesin-8 Klp5 and Klp6 are interdependent for mitotic nuclear retention and required for proper microtubule dynamics.

Unsworth A et al. Mol Biol Cell 2008 Dec;19(12):5104-15

GO_REF:0000002 - Comments

PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.

Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .

Budden AM et al. Elife 2023 Aug 24;12

PMID:8119981 - Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression.

Nishi K et al. J Biol Chem 1994 Mar 04;269(9):6320-4

PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183

PMID:9585505 - Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1.

Toone WM et al. Genes Dev 1998 May 15;12(10):1453-63

PMID:10588642 - Nuclear localization of Schizosaccharomyces pombe Mcm2/Cdc19p requires MCM complex assembly.

Pasion SG et al. Mol Biol Cell 1999 Dec;10(12):4043-57

PMID:10930468 - Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeast.

Paoletti A et al. Mol Biol Cell 2000 Aug;11(8):2757-73

PMID:10233152 - Active nucleocytoplasmic shuttling required for function and regulation of stress-activated kinase Spc1/StyI in fission yeast.

Gaits F et al. Mol Biol Cell 1999 May;10(5):1395-407

PMID:9211790 - Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.

Benko Z et al. Curr Genet 1997 Jun;31(6):481-7

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:29958883 - Role of nucleocytoplasmic transport in interphase microtubule organization in fission yeast.

Kume K et al. Biochem Biophys Res Commun 2018 Sep 05;503(2):1160-1167

PMID:19696784 - Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast.

Sato M et al. EMBO Rep 2009 Oct;10(10):1161-7

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:12376568 - An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin.

Tange Y et al. J Cell Sci 2002 Nov 15;115(Pt 22):4375-85

GO_REF:0000051 - S. pombe keyword mapping

PMID:7929079 - Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistance.

Turi TG et al. J Biol Chem 1994 Sep 30;269(39):24229-36