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protein coding gene - erf1 (SPAC1834.01) - cytoplasmic translation release factor class I eRF1

Gene summary

Standard name
erf1
Systematic ID
SPAC1834.01
Product
cytoplasmic translation release factor class I eRF1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
sup45
UniProt ID
P79063
ORFeome ID
32/32G12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4691780..4693738 forward strand

Annotation

GO biological process

GO:0002184 - cytoplasmic translational termination

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0018444 - translation release factor complex

References:

GO molecular function

GO:0030695 - GTPase regulator activity

References:

GO:0005515 - protein binding

References:

GO:1990825 - sequence-specific mRNA binding

References:

GO:0003747 - translation release factor activity

References:

GO:0016149 - translation release factor activity, codon specific

References:

Modification

MOD:00427 - methylated residue

References:

MOD:00080 - N5-methyl-L-glutamine

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000750 - sensitive to silver ions

References:

Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03463eRF1_1eRF1_Pelota-like_NPfam
PF03464eRF1_2eRF1_2Pfam
PF03465eRF1_3eRF1_3Pfam
SM01194eRF1_1eRF1_Pelota-like_NSMART
G3DSA:3.30.1330.30:FF:000006CATH-FunFam
G3DSA:3.30.420.60:FF:000001CATH-FunFam
G3DSA:3.30.960.10:FF:000002CATH-FunFam
G3DSA:3.30.1330.30Ribosomal_eL30-like_sfCATH-Gene3D
G3DSA:3.30.420.60eFR1_2_sfCATH-Gene3D
G3DSA:3.30.960.10eRF1_1_sfCATH-Gene3D
SSF53137SUPERFAMILY
SSF55315Ribosomal_eL30-like_sfSUPERFAMILY
SSF55481eRF1_1_sfSUPERFAMILY
PTHR10113Peptide_chain-rel_eRF1/aRF1PANTHER
TIGR03676aRF1_eRF1Peptide_chain-rel_eRF1/aRF1NCBIFAM

Orthologs

References / Literature

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:15576681 - Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.
Tanaka H et al. Nucleic Acids Res 2004;32(21):6367-77
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:19366728 - Genetic control of cellular quiescence in S. pombe.
Sajiki K et al. J Cell Sci 2009 May 01;122(Pt 9):1418-29
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20890290 - Structure of the Dom34-Hbs1 complex and implications for no-go decay.
Chen L et al. Nat Struct Mol Biol 2010 Oct;17(10):1233-40
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:19417105 - Structural insights into eRF3 and stop codon recognition by eRF1.
Cheng Z et al. Genes Dev 2009 May 01;23(9):1106-18
PMID:15099522 - Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe.
Kong C et al. Mol Cell 2004 Apr 23;14(2):233-45
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:9701287 - The stretch of C-terminal acidic amino acids of translational release factor eRF1 is a primary binding site for eRF3 of fission yeast.
Ito K et al. RNA 1998 Aug;4(8):958-72
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105