protein coding gene - SPAC186.03 - L-asparaginase

Gene summary

Systematic ID: SPAC186.03
Product: L-asparaginase
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: P87015
ORFeome ID: 16/16H02
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 5535179..5536432 forward strand

Annotation

GO biological process

GO:0006530 - L-asparagine catabolic process

References:

GO_REF:0000033

GO cellular component

GO:0009986 - cell surface

References:

GO_REF:0000051

GO:0042597 - periplasmic space

References:

GO_REF:0000033

GO molecular function

GO:0004067 - asparaginase activity

References:

GO_REF:0000033

Modification

MOD:00006 - N-glycosylated residue

References:

PMID:36408920

Protein sequence feature

SO:0000418 - signal_peptide

References:

PMID:36408920

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PMID:39705284

PomGeneEx:0000027 - ribosomal density decreased

References:

PMID:29432178

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

PMID:29259000

Genotypes:

SPAC186.03delta/SPAC186.03delta

FYPO:0003938 - increased cell population growth during glucose starvation

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0009036 - resistance to benzamidine

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0000763 - resistance to cadmium

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0009038 - resistance to egtazic acid

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

SPAC186.03delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

SPAC186.03delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

SPAC186.03delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

ID	Name	InterPro name	DB name
PF17763	Asparaginase_C	Asparaginase_C	PFAM
PF00710	Asparaginase	L-asparaginase_N	PFAM
cd08964	L-asparaginase_II	AsnASE_II	CDD
PS51732	ASN_GLN_ASE_3	Asparaginase/glutaminase-like	PROSITE_PROFILES
SM00870	Asparaginase_2	Asparaginase/glutaminase-like	SMART
PR00139	ASNGLNASE	Asparaginase/glutaminase-like	PRINTS
G3DSA:3.40.50.40:FF:000006			FUNFAM
G3DSA:3.40.50.1170:FF:000001			FUNFAM
SSF53774	Glutaminase/Asparaginase	Asp/glu_Ase-like_sf	SUPERFAMILY
G3DSA:3.40.50.1170		L-asparaginase_N_sf	GENE3D
G3DSA:3.40.50.40		L-asparaginase_C	GENE3D
PTHR11707	L-ASPARAGINASE	Asparaginase/glutaminase-like	PANTHER
PIRSF001220	L-ASNase_gatD	Asparaginase/glutaminase-like	PIRSF
PIRSF500176	L_ASNase		PIRSF
TIGR00520	asnASE_II	AsnASE_II	NCBIFAM

Orthologs

ASPG (HGNC:20123) Homo sapiens
ASP3-1 (YLR155C) Saccharomyces cerevisiae
ASP3-2 (YLR157C) Saccharomyces cerevisiae
ASP3-3 (YLR158C) Saccharomyces cerevisiae
ASP3-4 (YLR160C) Saccharomyces cerevisiae

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22840777 - Analyzing fission yeast multidrug resistance mechanisms to develop a genetically tractable model system for chemical biology.: Kawashima SA et al. Chem Biol 2012 Jul 27;19(7):893-901
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.: Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.: Blyth J et al. Genetics 2018 Feb;208(2):589-603
GO_REF:0000051 - S. pombe keyword mapping
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.: UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.: Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623