PomBase home

protein coding gene - pus9 (SPAC18B11.02c) - tRNA pseudouridine(32) synthase Pus9

Gene summary

Standard name
pus9
Systematic ID
SPAC18B11.02c
Product
tRNA pseudouridine(32) synthase Pus9
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09709
ORFeome ID
18/18G09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 313979..315335 forward strand

Annotation

GO biological process

GO:0000455 - enzyme-directed rRNA pseudouridine synthesis

References:

GO:0031119 - tRNA pseudouridine synthesis

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005739 - mitochondrion

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003723 - RNA binding

References:

GO:0160151 - tRNA pseudouridine(32) synthase activity

References:

Modification

MOD:00048 - O4'-phospho-L-tyrosine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000084 - sensitive to 6-azauracil

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00849PseudoU_synth_2PsdUridine_synth_RsuA/RluAPfam
cd02557PseudoU_synth_ScRIB2CDD
PS01129PSI_RLUPsdUridine_synth_RluA-like_CSPROSITE patterns
G3DSA:3.30.2350.10:FF:000017CATH-FunFam
G3DSA:3.30.2350.10CATH-Gene3D
SSF55120PsdUridine_synth_cat_dom_sfSUPERFAMILY
PTHR21600RluA_PseudoU_synthasePANTHER
TIGR00005rluA_subfamPsdUridine_synth_RluC/DNCBIFAM

Orthologs

References / Literature

PMID:26173815 - Genome-wide screen of fission yeast mutants for sensitivity to 6-azauracil, an inhibitor of transcriptional elongation.
Zhou H et al. Yeast 2015 Oct;32(10):643-55
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000002 - Comments
PMID:40829803 - MoDorado: enhanced detection of tRNA modifications in nanopore sequencing by off-label use of modification callers.
Rübsam FNM et al. Nucleic Acids Res 2025 Aug 11;53(15)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240