protein coding gene - rhp6 (SPAC18B11.07c) - histone H2B-K119 ubiquitin ligase complex (HULC), ubiquitin conjugating enzyme E2 subunit Rhp6/Rad6

Gene summary

Standard name

rhp6

Systematic ID

SPAC18B11.07c

Product

histone H2B-K119 ubiquitin ligase complex (HULC), ubiquitin conjugating enzyme E2 subunit Rhp6/Rad6

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

ubc2, sng1, rad6

UniProt ID

P23566

ORFeome ID

11/11F12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 305606..306762 forward strand

Annotation

Protein features

Orthologs

• UBE2A (HGNC:12472) Homo sapiens
• UBE2B (HGNC:12473) Homo sapiens
• rhp6 (SJAG_00293) Schizosaccharomyces japonicus
• RAD6 (YGL058W) Saccharomyces cerevisiae

References / Literature

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:22017871 - Regulation of the Sre1 hypoxic transcription factor by oxygen-dependent control of DNA binding.

Lee CY et al. Mol Cell 2011 Oct 21;44(2):225-34

PMID:9755190 - Histone deacetylase homologs regulate epigenetic inheritance of transcriptional silencing and chromosome segregation in fission yeast.

Grewal SI et al. Genetics 1998 Oct;150(2):563-76

PMID:11702950 - Phosphorylation of Mei2 and Ste11 by Pat1 kinase inhibits sexual differentiation via ubiquitin proteolysis and 14-3-3 protein in fission yeast.

Kitamura K et al. Dev Cell 2001 Sep;1(3):389-99

PMID:23209828 - Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor.

Penney M et al. PLoS One 2012;7(11):e50796

PMID:28162934 - S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity.

Lv Z et al. Mol Cell 2017 Feb 16;65(4):699-714.e6

PMID:10224243 - Caffeine-mediated override of checkpoint controls. A requirement for rhp6 (Schizosaccharomyces pombe).

Rowley R et al. Genetics 1999 May;152(1):61-71

PMID:11523791 - A homologue of the Rad18 postreplication repair gene is required for DNA damage responses throughout the fission yeast cell cycle.

Verkade HM et al. Mol Genet Genomics 2001 Aug;265(6):993-1003

PMID:16096059 - Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8.

Takeda K et al. Cell 2005 Aug 12;122(3):393-405

PMID:27098497 - CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.

Zeng M et al. Nat Commun 2016 Apr 21;7:11364

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:17374714 - Ubiquitylation of histone H2B controls RNA polymerase II transcription elongation independently of histone H3 methylation.

Tanny JC et al. Genes Dev 2007 Apr 01;21(7):835-47

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.

Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)

PMID:12417737 - Two ubiquitin-conjugating enzymes, Rhp6 and UbcX, regulate heterochromatin silencing in Schizosaccharomyces pombe.

Choi ES et al. Mol Cell Biol 2002 Dec;22(23):8366-74

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Mojardín L et al. Cell Cycle 2015;14(2):206-18

PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721

PMID:9154838 - A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.

Osaka F et al. Mol Cell Biol 1997 Jun;17(6):3388-97

PMID:7254221 - Spontaneous and UV-induced recombination in radiation-sensitive mutants of Schizosaccharomyces pombe.

Grossenbacher-Grunder AM et al. Mutat Res 1981 Mar;81(1):37-48

PMID:23738021 - A genome-wide screening of potential target genes to enhance the antifungal activity of micafungin in Schizosaccharomyces pombe.

Zhou X et al. PLoS One 2013;8(5):e65904

PMID:24100010 - Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast.

DeGennaro CM et al. Mol Cell Biol 2013 Dec;33(24):4779-92

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:25356590 - Multifaceted genome control by Set1 Dependent and Independent of H3K4 methylation and the Set1C/COMPASS complex.

Mikheyeva IV et al. PLoS Genet 2014 Oct;10(10):e1004740

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:35075549 - Schizosaccharomyces pombe Fzo1 is subjected to the ubiquitin-proteasome-mediated degradation during the stationary phase.

Ahmad F et al. Int Microbiol 2022 May;25(2):397-404

PMID:166019 - Genetic control of radiation sensitivity in Schizosaccharomyces pombe.

Nasim A et al. Genetics 1975 Apr;79(4):573-82

PMID:17611416 - Bin1 homolog hob1 supports a Rad6-Set1 pathway of transcriptional repression in fission yeast.

Ramalingam A et al. Cell Cycle 2007 Jul 01;6(13):1655-62

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:17456468 - The plant homeodomain fingers of fission yeast Msc1 exhibit E3 ubiquitin ligase activity.

Dul BE et al. J Biol Chem 2007 Jun 22;282(25):18397-18406

PMID:14993467 - The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.

Doe CL et al. Nucleic Acids Res 2004;32(4):1480-91

PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.

Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:17363370 - HULC, a histone H2B ubiquitinating complex, modulates heterochromatin independent of histone methylation in fission yeast.

Zofall M et al. J Biol Chem 2007 May 11;282(19):14065-72

PMID:2184030 - The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.

Reynolds P et al. EMBO J 1990 May;9(5):1423-30

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:12511578 - Identification of Uhp1, a ubiquitinated histone-like protein, as a target/mediator of Rhp6 in mating-type silencing in fission yeast.

Naresh A et al. J Biol Chem 2003 Mar 14;278(11):9185-94

PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.

Vega M et al. BMC Biol 2022 Jul 12;20(1):160

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:23348717 - Essential role of Ubr11, but not Ubr1, as an N-end rule ubiquitin ligase in Schizosaccharomyces pombe.

Fujiwara H et al. Yeast 2013 Jan;30(1):1-11

PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.

Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515

PMID:28366642 - Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.

Shetty A et al. Mol Cell 2017 Apr 06;66(1):77-88.e5

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:35908934 - Euchromatin factors HULC and Set1C affect heterochromatin organization and mating-type switching in fission yeast Schizosaccharomyces pombe.

Esquivel-Chávez A et al. Genes Genet Syst 2022 Oct 18;97(3):123-138

PMID:38718864 - H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.

Qin B et al. J Biol Chem 2024 May 06;:107345

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:23416107 - Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.

Olsen SK et al. Mol Cell 2013 Mar 07;49(5):884-96

PMID:9658208 - Sensitivity to cisplatin and platinum-containing compounds of Schizosaccharomyces pombe rad mutants.

Perego P et al. Mol Pharmacol 1998 Jul;54(1):213-9

PMID:9710635 - A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast.

Singh J et al. Mol Cell Biol 1998 Sep;18(9):5511-22