protein coding gene - chp1 (SPAC18G6.02c) - heterochromatin (HP1) family chromodomain protein Chp1

Gene summary

Standard name

chp1

Systematic ID

SPAC18G6.02c

Product

heterochromatin (HP1) family chromodomain protein Chp1

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q10103

ORFeome ID

47/47D04

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2210810..2214345 reverse strand

Annotation

Protein features

Orthologs

• chp1 (SJAG_03916) Schizosaccharomyces japonicus

References / Literature

PMID:9722643 - The fission yeast chromo domain encoding gene chp1(+) is required for chromosome segregation and shows a genetic interaction with alpha-tubulin.

Doe CL et al. Nucleic Acids Res 1998 Sep 15;26(18):4222-9

PMID:19164572 - Msc1 links dynamic Swi6/HP1 binding to cell fate determination.

Lawrence RJ et al. Proc Natl Acad Sci U S A 2009 Jan 27;106(4):1163-8

PMID:18345014 - Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.

Zhang K et al. Nat Struct Mol Biol 2008 Apr;15(4):381-8

PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.

Okita AK et al. Commun Biol 2019;2:17

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.

Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90

PMID:27648579 - A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading.

Shan CM et al. Elife 2016 Sep 20;5

PMID:21436456 - Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.

Zhang K et al. Science 2011 Mar 25;331(6024):1624-7

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:20062003 - Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast.

Simmer F et al. EMBO Rep 2010 Feb;11(2):112-8

PMID:33693625 - Dri1 mediates heterochromatin assembly via RNAi and histone deacetylation.

Ban H et al. Genetics 2021 May 17;218(1)

PMID:40132111 - A fission yeast CENP-B homologue Abp1 prevents RNAi-mediated heterochromatin formation at ribosomal DNA repeats.

Tsunemine S et al. Genetics 2025 Mar 25;

PMID:22522705 - Mmi1 RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeast.

Hiriart E et al. EMBO J 2012 May 16;31(10):2296-308

PMID:15537537 - Heterochromatin regulates cell type-specific long-range chromatin interactions essential for directed recombination.

Jia S et al. Cell 2004 Nov 12;119(4):469-80

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:26443059 - Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast.

Deng X et al. Sci Rep 2015 Oct 07;5:14707

PMID:16246721 - The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast.

Yamada T et al. Mol Cell 2005 Oct 28;20(2):173-85

PMID:17531816 - Functional separation of the requirements for establishment and maintenance of centromeric heterochromatin.

Partridge JF et al. Mol Cell 2007 May 25;26(4):593-602

PMID:22081013 - The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.

Schalch T et al. Nat Struct Mol Biol 2011 Nov 13;18(12):1351-7

PMID:29136238 - Regulation of transcriptional silencing and chromodomain protein localization at centromeric heterochromatin by histone H3 tyrosine 41 phosphorylation in fission yeast.

Ren B et al. Nucleic Acids Res 2018 Jan 09;46(1):189-202

PMID:24662054 - The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.

Creamer KM et al. Mol Cell Biol 2014 Jun;34(11):2046-61

PMID:21289066 - Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.

Reddy BD et al. Genes Dev 2011 Feb 01;25(3):214-9

PMID:26744419 - Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2.

Barrales RR et al. Genes Dev 2016 Jan 15;30(2):133-48

PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.

Takeda K et al. PLoS One 2011;6(7):e22021

PMID:20705239 - The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.

Gerace EL et al. Mol Cell 2010 Aug 13;39(3):360-72

PMID:22768388 - Translational control of cell division by Elongator.

Bauer F et al. Cell Rep 2012 May 31;1(5):424-33

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:15743828 - RNA interference (RNAi)-dependent and RNAi-independent association of the Chp1 chromodomain protein with distinct heterochromatic loci in fission yeast.

Petrie VJ et al. Mol Cell Biol 2005 Mar;25(6):2331-46

PMID:15372076 - A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast.

Sadaie M et al. EMBO J 2004 Oct 01;23(19):3825-35

PMID:38048463 - Rex1BD and the 14-3-3 protein control heterochromatin organization at tandem repeats by linking RNAi and HDAC.

Gao J et al. Proc Natl Acad Sci U S A 2023 Dec 12;120(50):e2309359120

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:16428807 - Hip3 interacts with the HIRA proteins Hip1 and Slm9 and is required for transcriptional silencing and accurate chromosome segregation.

Greenall A et al. J Biol Chem 2006 Mar 31;281(13):8732-9

PMID:23478021 - Replication fork stability is essential for the maintenance of centromere integrity in the absence of heterochromatin.

Li PC et al. Cell Rep 2013 Mar 28;3(3):638-45

PMID:15615848 - RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production.

Sugiyama T et al. Proc Natl Acad Sci U S A 2005 Jan 04;102(1):152-7

PMID:17289569 - SHREC, an effector complex for heterochromatic transcriptional silencing.

Sugiyama T et al. Cell 2007 Feb 09;128(3):491-504

PMID:22144463 - RNA elimination machinery targeting meiotic mRNAs promotes facultative heterochromatin formation.

Zofall M et al. Science 2012 Jan 06;335(6064):96-100

PMID:14704433 - RNAi-mediated targeting of heterochromatin by the RITS complex.

Verdel A et al. Science 2004 Jan 30;303(5658):672-6

PMID:10835380 - Four chromo-domain proteins of Schizosaccharomyces pombe differentially repress transcription at various chromosomal locations.

Thon G et al. Genetics 2000 Jun;155(2):551-68

PMID:19362535 - High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin.

Schalch T et al. Mol Cell 2009 Apr 10;34(1):36-46

PMID:20178743 - Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.

Halic M et al. Cell 2010 Feb 19;140(4):504-16

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:11069763 - Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.

Pidoux AL et al. J Cell Sci 2000 Dec;113 Pt 23:4177-91

PMID:26173815 - Genome-wide screen of fission yeast mutants for sensitivity to 6-azauracil, an inhibitor of transcriptional elongation.

Zhou H et al. Yeast 2015 Oct;32(10):643-55

PMID:27538348 - Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.

Yu Y et al. Sci Rep 2016 Aug 19;6:31752

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:28682306 - Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription.

Jih G et al. Nature 2017 Jul 27;547(7664):463-467

PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47

PMID:31255284 - Nuclear aconitase antagonizes heterochromatic silencing by interfering with Chp1 binding to DNA.

Jung SJ et al. Biochem Biophys Res Commun 2019 Aug 27;516(3):806-811

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:29237752 - Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi.

Kajitani T et al. Proc Natl Acad Sci U S A 2017 Dec 26;114(52):E11208-E11217

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.

Bayne EH et al. Genome Biol 2014;15(10):481

PMID:27462451 - The Chp1 chromodomain binds the H3K9me tail and the nucleosome core to assemble heterochromatin.

Zocco M et al. Cell Discov 2016;2:16004

PMID:15475954 - RITS acts in cis to promote RNA interference-mediated transcriptional and post-transcriptional silencing.

Noma K et al. Nat Genet 2004 Nov;36(11):1174-80

PMID:22895252 - A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.

Kawakami K et al. Genes Dev 2012 Aug 15;26(16):1811-24

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:36617881 - Tandemly repeated genes promote RNAi-mediated heterochromatin formation via an antisilencing factor, Epe1, in fission yeast.

Asanuma T et al. Genes Dev 2022 Nov;36(21-24):1145-1159

PMID:31000521 - Overlapping Roles in Chromosome Segregation for Heterochromatin Protein 1 (Swi6) and DDK in Schizosaccharomyces pombe .

Shen KF et al. Genetics 2019 Jun;212(2):417-430

PMID:15607976 - Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.

Motamedi MR et al. Cell 2004 Dec 17;119(6):789-802

PMID:22474355 - Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

Hayashi A et al. Proc Natl Acad Sci U S A 2012 Apr 17;109(16):6159-64

PMID:24095277 - Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.

Marasovic M et al. Mol Cell 2013 Oct 24;52(2):173-83

PMID:10766735 - Distinct protein interaction domains and protein spreading in a complex centromere.

Partridge JF et al. Genes Dev 2000 Apr 01;14(7):783-91

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PMID:26832414 - Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex.

He H et al. Cell Rep 2016 Feb 09;14(5):1018-1024

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:22990236 - Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin.

Shim YS et al. EMBO J 2012 Nov 28;31(23):4375-87

PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

GO_REF:0000002 - Comments

PMID:22733737 - Ers1 links HP1 to RNAi.

Rougemaille M et al. Proc Natl Acad Sci U S A 2012 Jul 10;109(28):11258-63

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:24240238 - Elimination of shelterin components bypasses RNAi for pericentric heterochromatin assembly.

Tadeo X et al. Genes Dev 2013 Nov 15;27(22):2489-99

PMID:34010645 - The histone H3K9M mutation synergizes with H3K14 ubiquitylation to selectively sequester histone H3K9 methyltransferase Clr4 at heterochromatin.

Shan CM et al. Cell Rep 2021 May 18;35(7):109137

PMID:17310250 - Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.

Buker SM et al. Nat Struct Mol Biol 2007 Mar;14(3):200-7

PMID:20299449 - A chromodomain switch mediated by histone H3 Lys 4 acetylation regulates heterochromatin assembly.

Xhemalce B et al. Genes Dev 2010 Apr 01;24(7):647-52

PMID:32269268 - Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin.

Dong W et al. Sci Rep 2020 Apr 08;10(1):6055

PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.

Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:20211136 - Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity.

Bayne EH et al. Cell 2010 Mar 05;140(5):666-77

PMID:22727667 - Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing.

Ishida M et al. Mol Cell 2012 Jul 27;47(2):228-41

PMID:37279920 - A dual, catalytic role for the fission yeast Ccr4-Not complex in gene silencing and heterochromatin spreading.

Challal D et al. Genetics 2023 Aug 09;224(4)

PMID:25972440 - Global regulation of heterochromatin spreading by Leo1.

Verrier L et al. Open Biol 2015 May;5(5)