protein coding gene - mal3 (SPAC18G6.15) - microtubule plus-end binding protein, EB1 family Mal3

Gene summary

Standard name

mal3

Systematic ID

SPAC18G6.15

Product

microtubule plus-end binding protein, EB1 family Mal3

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q10113

ORFeome ID

14/14C11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2243615..2245220 forward strand

Annotation

Protein features

Orthologs

• MAPRE1 (HGNC:6890) Homo sapiens
• MAPRE2 (HGNC:6891) Homo sapiens
• mal3 (SJAG_02045) Schizosaccharomyces japonicus
• BIM1 (YER016W) Saccharomyces cerevisiae

References / Literature

PMID:23755176 - Proper microtubule structure is vital for timely progression through meiosis in fission yeast.

Yamashita A et al. PLoS One 2013;8(6):e65082

PMID:32441227 - Glucose limitation and pka1 deletion rescue aberrant mitotic spindle formation induced by Mal3 overexpression in Schizosaccharomyces pombe .

Tanabe T et al. Biosci Biotechnol Biochem 2020 Aug;84(8):1667-1680

PMID:33946513 - The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.

Yukawa M et al. Int J Mol Sci 2021 Apr 30;22(9)

PMID:34080538 - Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.

Krüger LK et al. Elife 2021 Jun 03;10

PMID:15177031 - Tea2p kinesin is involved in spatial microtubule organization by transporting tip1p on microtubules.

Busch KE et al. Dev Cell 2004 Jun;6(6):831-43

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:16951255 - S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner.

Grallert A et al. Genes Dev 2006 Sep 01;20(17):2421-36

PMID:22137473 - Shaping fission yeast cells by rerouting actin-based transport on microtubules.

Lo Presti L et al. Curr Biol 2011 Dec 20;21(24):2064-9

PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.

Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43

PMID:27872152 - An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.

Matsuo Y et al. J Cell Sci 2016 Dec 15;129(24):4592-4606

PMID:29316864 - Overexpression of the transcription factor Rst2 in Schizosaccharomyces pombe indicates growth defect, mitotic defects, and microtubule disorder.

Takenaka K et al. Biosci Biotechnol Biochem 2018 Feb;82(2):247-257

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:35293864 - Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B.

Lera-Ramirez M et al. Elife 2022 Mar 16;11

PMID:36980258 - Actin-Microtubule Crosstalk Imparts Stiffness to the Contractile Ring in Fission Yeast.

Bellingham-Johnstun K et al. Cells 2023 Mar 16;12(6)

PMID:17895368 - Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth.

Alvarez-Tabarés I et al. J Cell Sci 2007 Oct 15;120(Pt 20):3589-601

PMID:31483748 - Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.

Zhurinsky J et al. Mol Biol Cell 2019 Oct 01;30(21):2695-2708

PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.

Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:19542312 - Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.

Dodgson J et al. Eukaryot Cell 2009 Aug;8(8):1298-306

PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.

Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:18794845 - Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice.

des Georges A et al. Nat Struct Mol Biol 2008 Oct;15(10):1102-8

PMID:20498706 - Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.

Goldstone S et al. PLoS One 2010 May 13;5(5):e10634

PMID:24039245 - The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.

Braun M et al. Cytoskeleton (Hoboken) 2013 Sep;70(9):515-21

PMID:11102508 - A conserved interaction between Moe1 and Mal3 is important for proper spindle formation in Schizosaccharomyces pombe.

Chen CR et al. Mol Biol Cell 2000 Dec;11(12):4067-77

PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.

Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239

PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.

Vega M et al. BMC Biol 2022 Jul 12;20(1):160

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:23128140 - Microtubule-binding sites of the CH domain of EB1 and its autoinhibition revealed by NMR.

Kanaba T et al. Biochim Biophys Acta 2013 Feb;1834(2):499-507

PMID:25210736 - Dynamics of cell shape inheritance in fission yeast.

Abenza JF et al. PLoS One 2014;9(9):e106959

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.

Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904

PMID:12242294 - The mal2p protein is an essential component of the fission yeast centromere.

Jin QW et al. Mol Cell Biol 2002 Oct;22(20):7168-83

PMID:15062095 - The microtubule plus end-tracking proteins mal3p and tip1p cooperate for cell-end targeting of interphase microtubules.

Busch KE et al. Curr Biol 2004 Apr 06;14(7):548-59

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:11086011 - The fission yeast ran GTPase is required for microtubule integrity.

Fleig U et al. J Cell Biol 2000 Nov 27;151(5):1101-11

EMD:3522 - Unknown title

PMID:23051734 - Myosin Vs organize actin cables in fission yeast.

Lo Presti L et al. Mol Biol Cell 2012 Dec;23(23):4579-91

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:16179942 - Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment.

Asakawa K et al. EMBO Rep 2005 Dec;6(12):1194-200

PMID:20624911 - Asp1, a conserved 1/3 inositol polyphosphate kinase, regulates the dimorphic switch in Schizosaccharomyces pombe.

Pöhlmann J et al. Mol Cell Biol 2010 Sep;30(18):4535-47

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:36536175 - Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends.

Maan R et al. Nat Cell Biol 2023 Jan;25(1):68-78

PMID:17190604 - The Schizosaccharomyces pombe EB1 homolog Mal3p binds and stabilizes the microtubule lattice seam.

Sandblad L et al. Cell 2006 Dec 29;127(7):1415-24

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:24928430 - Fission yeast mtr1p regulates interphase microtubule cortical dwell-time.

Carlier-Grynkorn F et al. Biol Open 2014 Jun 13;3(7):591-6

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:26443240 - PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.

Kelkar M et al. Nat Commun 2015 Oct 07;6:8445

PMID:37099380 - Microtubule competition and cell growth recenter the nucleus after anaphase in fission yeast.

Bellingham-Johnstun K et al. Mol Biol Cell 2023 Jul 01;34(8):ar77

PMID:17561805 - Dynein participates in chromosome segregation in fission yeast.

Courtheoux T et al. Biol Cell 2007 Nov;99(11):627-37

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:18256284 - Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.

Gachet Y et al. Mol Biol Cell 2008 Apr;19(4):1646-62

PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.

Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99

PMID:30973898 - Mal3 is a multi-copy suppressor of the sensitivity to microtubule-depolymerizing drugs and chromosome mis-segregation in a fission yeast pka1 mutant.

Tanabe T et al. PLoS One 2019;14(4):e0214803

PMID:23087209 - The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning.

Mana-Capelli S et al. Mol Biol Cell 2012 Dec;23(23):4592-600

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:29235477 - Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.

von Loeffelholz O et al. Nat Commun 2017 Dec 13;8(1):2110

PMID:9348288 - Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form.

Beinhauer JD et al. J Cell Biol 1997 Nov 03;139(3):717-28

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.

Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643

PMID:23613905 - Electron tomography reveals novel microtubule lattice and microtubule organizing centre defects in +TIP mutants.

Höög JL et al. PLoS One 2013;8(4):e61698

PMID:30463883 - Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe .

Yukawa M et al. G3 (Bethesda) 2019 Jan 09;9(1):269-280

PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.

Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67

PMID:33925026 - Dual Impact of a Benzimidazole Resistant β-Tubulin on Microtubule Behavior in Fission Yeast.

Minagawa M et al. Cells 2021 Apr 28;10(5)

PMID:15665379 - The EB1 homolog Mal3 stimulates the ATPase of the kinesin Tea2 by recruiting it to the microtubule.

Browning H et al. J Biol Chem 2005 Apr 01;280(13):12299-304

PMID:22696680 - Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase.

Al-Bassam J et al. Mol Biol Cell 2012 Aug;23(15):2878-90

PMID:25254656 - The Vip1 inositol polyphosphate kinase family regulates polarized growth and modulates the microtubule cytoskeleton in fungi.

Pöhlmann J et al. PLoS Genet 2014 Sep;10(9):e1004586

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:39156640 - Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.

Ramos M et al. iScience 2024 Aug 16;27(8):110477

PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.

Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35

PMID:21336311 - Calcineurin ensures a link between the DNA replication checkpoint and microtubule-dependent polarized growth.

Kume K et al. Nat Cell Biol 2011 Mar;13(3):234-42

PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.

Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:20624975 - A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast.

Gao Q et al. Proc Natl Acad Sci U S A 2010 Jul 27;107(30):13330-5

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:24196444 - Mal3, the Schizosaccharomyces pombe homolog of EB1, is required for karyogamy and for promoting oscillatory nuclear movement during meiosis.

Polakova S et al. Cell Cycle 2014;13(1):72-7

PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433

PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.

Blyth J et al. Genetics 2018 Feb;208(2):589-603

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:12894167 - Targeted movement of cell end factors in fission yeast.

Browning H et al. Nat Cell Biol 2003 Sep;5(9):812-8

PMID:25869666 - Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.

Scheffler K et al. J Cell Biol 2015 Apr 13;209(1):47-58

PMID:29958883 - Role of nucleocytoplasmic transport in interphase microtubule organization in fission yeast.

Kume K et al. Biochem Biophys Res Commun 2018 Sep 05;503(2):1160-1167

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:38442865 - The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle.

Kume K et al. Open Biol 2024 Mar;14(3):230440

PMID:15837798 - Efficient formation of bipolar microtubule bundles requires microtubule-bound gamma-tubulin complexes.

Janson ME et al. J Cell Biol 2005 Apr 25;169(2):297-308

PMID:22134091 - A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules.

Iimori M et al. Exp Cell Res 2012 Feb 01;318(3):262-75

PMID:26124291 - A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.

Laporte D et al. J Cell Biol 2015 Jul 06;210(1):99-113

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:22500803 - EBs recognize a nucleotide-dependent structural cap at growing microtubule ends.

Maurer SP et al. Cell 2012 Apr 13;149(2):371-82