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protein coding gene - mal3 (SPAC18G6.15) - microtubule plus-end binding protein, EB1 family Mal3

Gene summary

Standard name
mal3
Systematic ID
SPAC18G6.15
Product
microtubule plus-end binding protein, EB1 family Mal3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10113
ORFeome ID
14/14C11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2243615..2245220 forward strand

Annotation

Complementation

PBO:0001436 - functionally complemented by human MAPRE1

Disease association

MONDO:0014755 - skin creases, congenital symmetric circumferential, 2

References:

GO biological process

GO:0051315 - attachment of mitotic spindle microtubules to kinetochore

References:

GO:0030989 - dynein-driven meiotic oscillatory nuclear movement

References:

GO:0000742 - karyogamy involved in conjugation with cellular fusion

References:

GO:0000743 - nuclear migration involved in conjugation with cellular fusion

References:

GO:0035372 - protein localization to microtubule

References:

GO:0031110 - regulation of microtubule polymerization or depolymerization

References:

GO:0051225 - spindle assembly

References:

GO cellular component

GO:0000235 - astral microtubule

References:

GO:0051285 - cell cortex of cell tip

References:

GO:0055028 - cortical microtubule

References:

GO:0005881 - cytoplasmic microtubule

References:

GO:0015630 - microtubule cytoskeleton

References:

GO:0035371 - microtubule plus-end

References:

GO:0061673 - mitotic spindle astral microtubule

References:

GO:1990023 - mitotic spindle midzone

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0005880 - nuclear microtubule

References:

GO:0005634 - nucleus

References:

GO:1905760 - post-anaphase array microtubule end

References:

GO molecular function

GO:0001671 - ATPase activator activity

References:

GO:0008093 - cytoskeletal adaptor activity

References:

GO:0008017 - microtubule binding

References:

GO:0099609 - microtubule lateral binding

References:

GO:0051010 - microtubule plus-end binding

References:

GO:0140693 - molecular condensate scaffold activity

References:

GO:0005515 - protein binding

References:

Miscellaneous functional group

PBO:0091364 - microtubule plus-end tracking protein

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0005381 - asymmetric gamma-tubulin complex localization to single mitotic spindle pole body

References:

Genotypes:

FYPO:0001978 - bent mitotic spindle

References:

Genotypes:

FYPO:0004700 - bent vegetative cell

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009001 - decreased duration of cytoplasmic microtubule growth events during mitotic interphase

References:

Genotypes:

FYPO:0006885 - decreased protein level at mitotic spindle

References:

Genotypes:

FYPO:0005494 - decreased protein transport along microtubule during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005684 - increased duration of mitotic prometaphase

References:

Genotypes:

FYPO:0005683 - increased duration of mitotic prophase

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0008233 - microtubule bundles present in normal numbers

References:

Genotypes:

FYPO:0000324 - mitotic metaphase/anaphase transition delay

References:

Genotypes:

FYPO:0001019 - monopolar actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0000276 - monopolar mitotic spindle

References:

Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

References:

Genotypes:

FYPO:0001383 - normal DNA content

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

FYPO:0001400 - normal interphase microtubules

References:

Genotypes:

FYPO:0007114 - normal microtubule bundle

References:

Genotypes:

FYPO:0001839 - normal minichromosome loss

References:

Genotypes:

FYPO:0001007 - normal mitosis

References:

Genotypes:

FYPO:0004367 - normal mitotic spindle assembly

References:

Genotypes:

FYPO:0005380 - normal mitotic spindle pole body duplication

References:

Genotypes:

FYPO:0007705 - normal protein localization to lateral cell cortex during glucose starvation

References:

Genotypes:

FYPO:0003185 - normal protein localization to microtubule cytoskeleton during mitotic interphase

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000069 - resistance to thiabendazole

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0006103 - short interphase microtubules

References:

Genotypes:

FYPO:0003429 - short interphase microtubules present in decreased numbers

References:

Genotypes:

FYPO:0005797 - short microtubule bundle

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0000945 - swollen spheroid vegetative cell

References:

Genotypes:

FYPO:0000013 - T-shaped vegetative cell with normal cell length

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111860 - calponin homology (CH) domain

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000608 - abnormal cell cycle arrest in mitotic M phase

References:

Genotypes:

FYPO:0000240 - abnormal filament morphology

References:

Genotypes:

FYPO:0000197 - abnormal horsetail movement

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0001943 - abnormal microtubule binding

References:

Genotypes:

FYPO:0003065 - abnormal microtubule cytoskeleton morphology during mating

References:

Genotypes:

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

References:

Genotypes:

FYPO:0000901 - abnormal microtubule dynamics during vegetative growth

References:

Genotypes:

FYPO:0002736 - abnormal mitotic cell cycle phase

References:

Genotypes:

FYPO:0000338 - abnormal mitotic spindle

References:

Genotypes:

FYPO:0000177 - abnormal mitotic spindle assembly

References:

Genotypes:

FYPO:0003304 - abnormal mitotic spindle midzone assembly

References:

Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

References:

Genotypes:

FYPO:0003263 - abnormal sporulation resulting in formation of ascus with more than four spores

References:

Genotypes:

FYPO:0001683 - abolished mitotic spindle assembly

References:

Genotypes:

FYPO:0007989 - abolished nuclear congression during mating

References:

Genotypes:

FYPO:0000511 - abolished nuclear fusion during mating

References:

Genotypes:

FYPO:0006502 - abolished protein localization to cell cortex of cell tip during vegetative growth

References:

Genotypes:

FYPO:0000938 - abolished protein localization to cortical microtubule cytoskeleton during mitotic interphase

References:

Genotypes:

FYPO:0003277 - abolished protein localization to microtubule during mitosis

References:

Genotypes:

FYPO:0005441 - abolished protein localization to microtubule during mitotic interphase

References:

Genotypes:

FYPO:0005814 - abolished protein localization to microtubule plus-end

References:

Genotypes:

FYPO:0002678 - abolished protein phosphorylation

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0009001 - decreased duration of cytoplasmic microtubule growth events during mitotic interphase

References:

Genotypes:

FYPO:0005681 - decreased microtubule polymerization

References:

Genotypes:

FYPO:0006885 - decreased protein level at mitotic spindle

References:

Genotypes:

FYPO:0007972 - decreased rate of interpolar microtubule polymerization during mitotic anaphase B

References:

Genotypes:

FYPO:0000903 - decreased rate of microtubule depolymerization during vegetative growth

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002636 - delayed onset of mitotic spindle elongation

References:

Genotypes:

FYPO:0003566 - delayed onset of mitotic spindle pole body separation

References:

Genotypes:

FYPO:0000158 - DNA content increased during vegetative growth

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001846 - increased duration of mitotic anaphase A

References:

Genotypes:

FYPO:0000274 - increased duration of mitotic M phase

References:

Genotypes:

FYPO:0007132 - increased microtubule binding

References:

Genotypes:

FYPO:0006180 - increased microtubule polymerization

References:

Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0001861 - increased minichromosome loss upon segregation during vegetative growth

References:

Genotypes:

FYPO:0003307 - increased mitotic index

References:

Genotypes:

FYPO:0003003 - increased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0007131 - increased protein localization to microtubule plus-end

References:

Genotypes:

FYPO:0002059 - inviable cell population

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0004439 - long curved mitotic spindle during anaphase

References:

Genotypes:

FYPO:0004611 - long interphase microtubules

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003061 - meiotic cell cycle in zygote with unfused nuclei

References:

Genotypes:

FYPO:0002818 - microtubule bundles present in decreased numbers

References:

Genotypes:

FYPO:0003303 - mislocalized post-anaphase array

References:

Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

References:

Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

References:

Genotypes:

FYPO:0000324 - mitotic metaphase/anaphase transition delay

References:

Genotypes:

FYPO:0000276 - monopolar mitotic spindle

References:

Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

References:

Genotypes:

FYPO:0002487 - normal actin cable morphology

References:

Genotypes:

FYPO:0003717 - normal actin cytoskeleton morphology during vegetative growth

References:

Genotypes:

FYPO:0001383 - normal DNA content

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

FYPO:0001400 - normal interphase microtubules

References:

Genotypes:

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

FYPO:0007114 - normal microtubule bundle

References:

Genotypes:

FYPO:0004367 - normal mitotic spindle assembly

References:

Genotypes:

FYPO:0004731 - normal protein localization to interphase microtubule plus-end

References:

Genotypes:

FYPO:0002901 - normal protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0002966 - normal protein localization to mitotic spindle

References:

Genotypes:

FYPO:0008003 - normal protein localization to site of mechanical stress

References:

Genotypes:

FYPO:0005827 - normal protein transport along microtubule during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0003302 - nucleus mislocalized towards cell tip during mitotic interphase

References:

Genotypes:

FYPO:0003264 - overlapping meiosis I spindles

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009066 - resistance to amorolfine

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0003595 - S-shaped cell

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0003305 - separate mitotic half spindles present

References:

Genotypes:

FYPO:0002760 - short cytoplasmic microtubules

References:

Genotypes:

FYPO:0006103 - short interphase microtubules

References:

Genotypes:

FYPO:0005797 - short microtubule bundle

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000013 - T-shaped vegetative cell with normal cell length

References:

Genotypes:

FYPO:0003245 - telophase nuclear clustering

References:

Genotypes:

FYPO:0000678 - unequal homologous chromosome segregation

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

References:

Genotypes:

FYPO:0002215 - viable curved elongated vegetative cell

References:

Genotypes:

FYPO:0002112 - viable curved vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF03271EB1EB1_CPfam
PS50021CHCH_domPROSITE profiles
PS51230EB1_CEB1_CPROSITE profiles
G3DSA:1.10.418.10:FF:000028CATH-FunFam
G3DSA:1.10.418.10CH_dom_sfCATH-Gene3D
G3DSA:1.20.5.1430CATH-Gene3D
SSF140612EB1_C_sfSUPERFAMILY
SSF47576CH_dom_sfSUPERFAMILY
PTHR10623MAPREPANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:24196444 - Mal3, the Schizosaccharomyces pombe homolog of EB1, is required for karyogamy and for promoting oscillatory nuclear movement during meiosis.
Polakova S et al. Cell Cycle 2014;13(1):72-7
PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.
Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43
PMID:33946513 - The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.
Yukawa M et al. Int J Mol Sci 2021 Apr 30;22(9)
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:15837798 - Efficient formation of bipolar microtubule bundles requires microtubule-bound gamma-tubulin complexes.
Janson ME et al. J Cell Biol 2005 Apr 25;169(2):297-308
PMID:22134091 - A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules.
Iimori M et al. Exp Cell Res 2012 Feb 01;318(3):262-75
PMID:18794845 - Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice.
des Georges A et al. Nat Struct Mol Biol 2008 Oct;15(10):1102-8
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:23613905 - Electron tomography reveals novel microtubule lattice and microtubule organizing centre defects in +TIP mutants.
Höög JL et al. PLoS One 2013;8(4):e61698
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:12894167 - Targeted movement of cell end factors in fission yeast.
Browning H et al. Nat Cell Biol 2003 Sep;5(9):812-8
PMID:20624975 - A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast.
Gao Q et al. Proc Natl Acad Sci U S A 2010 Jul 27;107(30):13330-5
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:30973898 - Mal3 is a multi-copy suppressor of the sensitivity to microtubule-depolymerizing drugs and chromosome mis-segregation in a fission yeast pka1 mutant.
Tanabe T et al. PLoS One 2019;14(4):e0214803
PMID:21336311 - Calcineurin ensures a link between the DNA replication checkpoint and microtubule-dependent polarized growth.
Kume K et al. Nat Cell Biol 2011 Mar;13(3):234-42
PMID:32441227 - Glucose limitation and pka1 deletion rescue aberrant mitotic spindle formation induced by Mal3 overexpression in Schizosaccharomyces pombe .
Tanabe T et al. Biosci Biotechnol Biochem 2020 Aug;84(8):1667-1680
PMID:17895368 - Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth.
Alvarez-Tabarés I et al. J Cell Sci 2007 Oct 15;120(Pt 20):3589-601
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22500803 - EBs recognize a nucleotide-dependent structural cap at growing microtubule ends.
Maurer SP et al. Cell 2012 Apr 13;149(2):371-82
PMID:20498706 - Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.
Goldstone S et al. PLoS One 2010 May 13;5(5):e10634
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23087209 - The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning.
Mana-Capelli S et al. Mol Biol Cell 2012 Dec;23(23):4592-600
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26124291 - A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.
Laporte D et al. J Cell Biol 2015 Jul 06;210(1):99-113
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:35293864 - Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B.
Lera-Ramirez M et al. Elife 2022 Mar 16;11
PMID:38442865 - The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle.
Kume K et al. Open Biol 2024 Mar;14(3):230440
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:11102508 - A conserved interaction between Moe1 and Mal3 is important for proper spindle formation in Schizosaccharomyces pombe.
Chen CR et al. Mol Biol Cell 2000 Dec;11(12):4067-77
PMID:15177031 - Tea2p kinesin is involved in spatial microtubule organization by transporting tip1p on microtubules.
Busch KE et al. Dev Cell 2004 Jun;6(6):831-43
PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.
Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67
PMID:17561805 - Dynein participates in chromosome segregation in fission yeast.
Courtheoux T et al. Biol Cell 2007 Nov;99(11):627-37
PMID:29958883 - Role of nucleocytoplasmic transport in interphase microtubule organization in fission yeast.
Kume K et al. Biochem Biophys Res Commun 2018 Sep 05;503(2):1160-1167
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:11086011 - The fission yeast ran GTPase is required for microtubule integrity.
Fleig U et al. J Cell Biol 2000 Nov 27;151(5):1101-11
PMID:23051734 - Myosin Vs organize actin cables in fission yeast.
Lo Presti L et al. Mol Biol Cell 2012 Dec;23(23):4579-91
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:31483748 - Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast.
Zhurinsky J et al. Mol Biol Cell 2019 Oct 01;30(21):2695-2708
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:24039245 - The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.
Braun M et al. Cytoskeleton (Hoboken) 2013 Sep;70(9):515-21
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.
Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23
PMID:22137473 - Shaping fission yeast cells by rerouting actin-based transport on microtubules.
Lo Presti L et al. Curr Biol 2011 Dec 20;21(24):2064-9
PMID:34080538 - Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.
Krüger LK et al. Elife 2021 Jun 03;10
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:15665379 - The EB1 homolog Mal3 stimulates the ATPase of the kinesin Tea2 by recruiting it to the microtubule.
Browning H et al. J Biol Chem 2005 Apr 01;280(13):12299-304
PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.
Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:12242294 - The mal2p protein is an essential component of the fission yeast centromere.
Jin QW et al. Mol Cell Biol 2002 Oct;22(20):7168-83
PMID:18256284 - Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.
Gachet Y et al. Mol Biol Cell 2008 Apr;19(4):1646-62
PMID:39156640 - Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.
Ramos M et al. iScience 2024 Aug 16;27(8):110477
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22696680 - Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase.
Al-Bassam J et al. Mol Biol Cell 2012 Aug;23(15):2878-90
PMID:25869666 - Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.
Scheffler K et al. J Cell Biol 2015 Apr 13;209(1):47-58
PMID:26443240 - PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
Kelkar M et al. Nat Commun 2015 Oct 07;6:8445
PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.
Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16179942 - Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment.
Asakawa K et al. EMBO Rep 2005 Dec;6(12):1194-200
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:30463883 - Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe .
Yukawa M et al. G3 (Bethesda) 2019 Jan 09;9(1):269-280
PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.
Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35
PMID:24928430 - Fission yeast mtr1p regulates interphase microtubule cortical dwell-time.
Carlier-Grynkorn F et al. Biol Open 2014 Jun 13;3(7):591-6
PMID:15062095 - The microtubule plus end-tracking proteins mal3p and tip1p cooperate for cell-end targeting of interphase microtubules.
Busch KE et al. Curr Biol 2004 Apr 06;14(7):548-59
PMID:16951255 - S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner.
Grallert A et al. Genes Dev 2006 Sep 01;20(17):2421-36
PMID:9348288 - Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form.
Beinhauer JD et al. J Cell Biol 1997 Nov 03;139(3):717-28
PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.
Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904
PMID:37099380 - Microtubule competition and cell growth recenter the nucleus after anaphase in fission yeast.
Bellingham-Johnstun K et al. Mol Biol Cell 2023 Jul 01;34(8):ar77
PMID:27872152 - An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.
Matsuo Y et al. J Cell Sci 2016 Dec 15;129(24):4592-4606
PMID:29235477 - Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.
von Loeffelholz O et al. Nat Commun 2017 Dec 13;8(1):2110
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:33925026 - Dual Impact of a Benzimidazole Resistant β-Tubulin on Microtubule Behavior in Fission Yeast.
Minagawa M et al. Cells 2021 Apr 28;10(5)
PMID:36980258 - Actin-Microtubule Crosstalk Imparts Stiffness to the Contractile Ring in Fission Yeast.
Bellingham-Johnstun K et al. Cells 2023 Mar 16;12(6)
PMID:25210736 - Dynamics of cell shape inheritance in fission yeast.
Abenza JF et al. PLoS One 2014;9(9):e106959
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:25254656 - The Vip1 inositol polyphosphate kinase family regulates polarized growth and modulates the microtubule cytoskeleton in fungi.
Pöhlmann J et al. PLoS Genet 2014 Sep;10(9):e1004586
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:36536175 - Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends.
Maan R et al. Nat Cell Biol 2023 Jan;25(1):68-78
PMID:23128140 - Microtubule-binding sites of the CH domain of EB1 and its autoinhibition revealed by NMR.
Kanaba T et al. Biochim Biophys Acta 2013 Feb;1834(2):499-507
PMID:17190604 - The Schizosaccharomyces pombe EB1 homolog Mal3p binds and stabilizes the microtubule lattice seam.
Sandblad L et al. Cell 2006 Dec 29;127(7):1415-24
PMID:19542312 - Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.
Dodgson J et al. Eukaryot Cell 2009 Aug;8(8):1298-306
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:20624911 - Asp1, a conserved 1/3 inositol polyphosphate kinase, regulates the dimorphic switch in Schizosaccharomyces pombe.
Pöhlmann J et al. Mol Cell Biol 2010 Sep;30(18):4535-47
PMID:29316864 - Overexpression of the transcription factor Rst2 in Schizosaccharomyces pombe indicates growth defect, mitotic defects, and microtubule disorder.
Takenaka K et al. Biosci Biotechnol Biochem 2018 Feb;82(2):247-257
PMID:23755176 - Proper microtubule structure is vital for timely progression through meiosis in fission yeast.
Yamashita A et al. PLoS One 2013;8(6):e65082