protein coding gene - rad1 (SPAC1952.07) - checkpoint clamp complex protein Rad1

Gene summary

Standard name

rad1

Systematic ID

SPAC1952.07

Product

checkpoint clamp complex protein Rad1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

rad19

UniProt ID

P22193

ORFeome ID

32/32E08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 4978578..4980303 forward strand

Annotation

Protein features

Orthologs

• RAD1 (HGNC:9806) Homo sapiens
• rad1 (SJAG_02771) Schizosaccharomyces japonicus
• RAD17 (YOR368W) Saccharomyces cerevisiae

References / Literature

PMID:15533944 - Interaction of checkpoint proteins Hus1/Rad1/Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast, Schizosaccharomyces pombe.

Chang DY et al. J Biol Chem 2005 Jan 07;280(1):408-17

PMID:12514100 - Checkpoint activation regulates mutagenic translesion synthesis.

Kai M et al. Genes Dev 2003 Jan 01;17(1):64-76

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:12196391 - Telomere binding of checkpoint sensor and DNA repair proteins contributes to maintenance of functional fission yeast telomeres.

Nakamura TM et al. Genetics 2002 Aug;161(4):1437-52

PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.

Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23

PMID:10954610 - The stress-activated MAP kinase Sty1/Spc1 and a 3'-regulatory element mediate UV-induced expression of the uvi15(+) gene at the post-transcriptional level.

Kim M et al. Nucleic Acids Res 2000 Sep 01;28(17):3392-402

PMID:12724426 - Role for the fission yeast RecQ helicase in DNA repair in G2.

Laursen LV et al. Mol Cell Biol 2003 May;23(10):3692-705

PMID:28441348 - Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication.

Fleck O et al. Genes (Basel) 2017 Apr 25;8(5)

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:10430579 - Genetic control of telomere integrity in Schizosaccharomyces pombe: rad3(+) and tel1(+) are parts of two regulatory networks independent of the downstream protein kinases chk1(+) and cds1(+).

Matsuura A et al. Genetics 1999 Aug;152(4):1501-12

PMID:10648611 - Characterization of Schizosaccharomyces pombe Hus1: a PCNA-related protein that associates with Rad1 and Rad9.

Caspari T et al. Mol Cell Biol 2000 Feb;20(4):1254-62

PMID:17426133 - The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair.

Gembka A et al. Nucleic Acids Res 2007;35(8):2596-608

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:8313892 - The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb.

Ohi R et al. EMBO J 1994 Jan 15;13(2):471-83

PMID:23677513 - A role for Myh1 in DNA repair after treatment with strand-breaking and crosslinking chemotherapeutic agents.

Jansson K et al. Environ Mol Mutagen 2013 Jun;54(5):327-37

PMID:11027257 - Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.

Tan S et al. Mol Cell Biol 2000 Nov;20(21):7853-66

PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47

PMID:8497322 - Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2.

Walworth N et al. Nature 1993 May 27;363(6427):368-71

PMID:9487130 - Regulation of telomere length by checkpoint genes in Schizosaccharomyces pombe.

Dahlen M et al. Mol Biol Cell 1998 Mar;9(3):611-21

PMID:7957098 - Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control.

Saka Y et al. EMBO J 1994 Nov 15;13(22):5319-29

PMID:8573795 - RACH2, a novel human gene that complements a fission yeast cell cycle checkpoint mutation.

Davey S et al. Mol Biol Cell 1995 Oct;6(10):1411-21

PMID:9739083 - Cdc18p can block mitosis by two independent mechanisms.

Greenwood E et al. J Cell Sci 1998 Oct;111 ( Pt 20):3101-8

PMID:9870697 - Differential effects of caffeine on DNA damage and replication cell cycle checkpoints in the fission yeast Schizosaccharomyces pombe.

Osman F et al. Mol Gen Genet 1998 Nov;260(4):319-34

PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

Dolan WP et al. Genetics 2010 May;185(1):39-53

PMID:22927644 - Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant.

Sánchez A et al. Mol Cell Biol 2012 Nov;32(21):4445-54

PMID:12186947 - A novel chk1-dependent G1/M checkpoint in fission yeast.

Synnes M et al. J Cell Sci 2002 Sep 15;115(Pt 18):3609-18

PMID:16371652 - Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.

Marchetti MA et al. J Cell Sci 2006 Jan 01;119(Pt 1):124-31

PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.

Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43

PMID:18378696 - Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex.

Akamatsu Y et al. Mol Cell Biol 2008 Jun;28(11):3639-51

PMID:8552194 - Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein.

Leatherwood J et al. Nature 1996 Jan 25;379(6563):360-3

PMID:166019 - Genetic control of radiation sensitivity in Schizosaccharomyces pombe.

Nasim A et al. Genetics 1975 Apr;79(4):573-82

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:10757807 - Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.

Wang SW et al. Mol Cell Biol 2000 May;20(9):3234-44

PMID:20444100 - Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis.

Alao JP et al. Mol Microbiol 2010 Jul 01;77(1):143-57

PMID:24587136 - The oxidative stress responsive transcription factor Pap1 confers DNA damage resistance on checkpoint-deficient fission yeast cells.

Belfield C et al. PLoS One 2014;9(2):e89936

PMID:8846774 - Fission yeast rad17: a homologue of budding yeast RAD24 that shares regions of sequence similarity with DNA polymerase accessory proteins.

Griffiths DJ et al. EMBO J 1995 Dec 01;14(23):5812-23

PMID:31538680 - The fission yeast FHIT homolog affects checkpoint control of proliferation and is regulated by mitochondrial electron transport.

Sjölander JJ et al. Cell Biol Int 2020 Feb;44(2):412-423

PMID:12062100 - Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA.

Saitoh S et al. Cell 2002 May 31;109(5):563-73

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:24815688 - Does a shift to limited glucose activate checkpoint control in fission yeast?

Saitoh S et al. FEBS Lett 2014 Aug 01;588(15):2373-8

PMID:2355921 - Cloning and analysis of a gene involved in DNA repair and recombination, the rad1 gene of Schizosaccharomyces pombe.

Sunnerhagen P et al. Mol Cell Biol 1990 Jul;10(7):3750-60

PMID:29121084 - Fission yeast strains with circular chromosomes require the 9-1-1 checkpoint complex for the viability in response to the anti-cancer drug 5-fluorodeoxyuridine.

Shamim HM et al. PLoS One 2017;12(11):e0187775

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:7723827 - A kinase from fission yeast responsible for blocking mitosis in S phase.

Murakami H et al. Nature 1995 Apr 27;374(6525):817-9

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:12409464 - The fission yeast pfh1(+) gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase.

Tanaka H et al. Nucleic Acids Res 2002 Nov 01;30(21):4728-39

PMID:7254221 - Spontaneous and UV-induced recombination in radiation-sensitive mutants of Schizosaccharomyces pombe.

Grossenbacher-Grunder AM et al. Mutat Res 1981 Mar;81(1):37-48

PMID:8631307 - Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle.

Maiorano D et al. EMBO J 1996 Feb 15;15(4):861-72

PMID:10407262 - The topoisomerase I poison camptothecin generates a Chk1-dependent DNA damage checkpoint signal in fission yeast.

Wan S et al. Yeast 1999 Jul;15(10A):821-8

PMID:12135745 - Cmk2, a novel serine/threonine kinase in fission yeast.

Alemany V et al. FEBS Lett 2002 Jul 31;524(1-3):79-86

PMID:1563349 - Checkpoint controls in Schizosaccharomyces pombe: rad1.

Rowley R et al. EMBO J 1992 Apr;11(4):1335-42

PMID:8842148 - Cell cycle, DNA damage and heat shock regulate suc22+ expression in fission yeast.

Harris P et al. Mol Gen Genet 1996 Sep 13;252(3):284-91

PMID:9154834 - Discrete roles of the Spc1 kinase and the Atf1 transcription factor in the UV response of Schizosaccharomyces pombe.

Degols G et al. Mol Cell Biol 1997 Jun;17(6):3356-63

PMID:9572736 - Replication checkpoint enforced by kinases Cds1 and Chk1.

Boddy MN et al. Science 1998 May 08;280(5365):909-12

PMID:9658208 - Sensitivity to cisplatin and platinum-containing compounds of Schizosaccharomyces pombe rad mutants.

Perego P et al. Mol Pharmacol 1998 Jul;54(1):213-9

PMID:9891039 - Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.

Tanaka H et al. Mol Cell Biol 1999 Feb;19(2):1038-48

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:31262821 - Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks.

Feng G et al. Proc Natl Acad Sci U S A 2019 Jul 16;116(29):14563-14572

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:9563836 - Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast.

Arroyo MP et al. Mol Gen Genet 1998 Mar;257(5):505-18

PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

Pan X et al. BMC Genomics 2012 Nov 23;13:662

PMID:14985109 - Cdk inhibitor ste9p/srw1p is involved in response to protein synthesis inhibition in fission yeast.

Sakai T Biochem Biophys Res Commun 2004 Mar 19;315(4):984-90

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:9649516 - Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity.

Gould KL et al. Genetics 1998 Jul;149(3):1221-33

PMID:12589755 - The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.

Kanoh J et al. J Mol Biol 2003 Feb 28;326(4):1081-94

PMID:21095590 - DDK phosphorylates checkpoint clamp component Rad9 and promotes its release from damaged chromatin.

Furuya K et al. Mol Cell 2010 Nov 24;40(4):606-18

PMID:9716408 - Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and Saccharomyces cerevisiae RAD17: linkage to checkpoint control and mammalian meiosis.

Freire R et al. Genes Dev 1998 Aug 15;12(16):2560-73

PMID:15498101 - Germinating fission yeast spores delay in G1 in response to UV irradiation.

Nilssen EA et al. BMC Cell Biol 2004 Oct 21;5(1):40

PMID:17690116 - Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast.

Fersht N et al. Nucleic Acids Res 2007;35(16):5323-37

PMID:9135148 - p56(chk1) protein kinase is required for the DNA replication checkpoint at 37 degrees C in fission yeast.

Francesconi S et al. EMBO J 1997 Mar 17;16(6):1332-41

PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Akai Y et al. Open Biol 2011 Dec;1(4):110023

PMID:12839619 - High dosage Rhp51 suppression of the MMS sensitivity of DNA structure checkpoint mutants reveals a relationship between Crb2 and Rhp51.

Smeets MF et al. Genes Cells 2003 Jul;8(7):573-86

PMID:21561865 - The phosphorylation network for efficient activation of the DNA replication checkpoint in fission yeast.

Yue M et al. J Biol Chem 2011 Jul 01;286(26):22864-74

PMID:9693370 - Mutational effect of fission yeast polalpha on cell cycle events.

Bhaumik D et al. Mol Biol Cell 1998 Aug;9(8):2107-23

PMID:12930957 - Nuclear factories for signalling and repairing DNA double strand breaks in living fission yeast.

Meister P et al. Nucleic Acids Res 2003 Sep 01;31(17):5064-73

PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.

Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73

PMID:10559981 - A Rad3-Rad26 complex responds to DNA damage independently of other checkpoint proteins.

Edwards RJ et al. Nat Cell Biol 1999 Nov;1(7):393-8

PMID:11030343 - Structure and function of Cdc6/Cdc18: implications for origin recognition and checkpoint control.

Liu J et al. Mol Cell 2000 Sep;6(3):637-48

PMID:10655214 - Functions of fission yeast orp2 in DNA replication and checkpoint control.

Kiely J et al. Genetics 2000 Feb;154(2):599-607

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:15340008 - Requirement for Schizosaccharomyces pombe Top3 in the maintenance of chromosome integrity.

Win TZ et al. J Cell Sci 2004 Sep 15;117(Pt 20):4769-78

PMID:18675827 - The tumor suppressor homolog in fission yeast, myh1(+), displays a strong interaction with the checkpoint gene rad1(+).

Jansson K et al. Mutat Res 2008 Sep 26;644(1-2):48-55

PMID:8590806 - Separation of phenotypes in mutant alleles of the Schizosaccharomyces pombe cell-cycle checkpoint gene rad1+.

Kanter-Smoler G et al. Mol Biol Cell 1995 Dec;6(12):1793-805

PMID:9524127 - Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage.

Kostrub CF et al. EMBO J 1998 Apr 01;17(7):2055-66

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:10521402 - Meiotic DNA replication checkpoint control in fission yeast.

Murakami H et al. Genes Dev 1999 Oct 01;13(19):2581-93

PMID:11809834 - Fission yeast F-box protein Pof3 is required for genome integrity and telomere function.

Katayama S et al. Mol Biol Cell 2002 Jan;13(1):211-24

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.

Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643

PMID:7926829 - The Schizosaccharomyces pombe rad1 gene consists of three exons and the cDNA sequence is partially homologous to the Ustilago maydis REC1 cDNA.

Long KE et al. Gene 1994 Oct 11;148(1):155-9

PMID:11073995 - Rdp1, a novel zinc finger protein, regulates the DNA damage response of rhp51(+) from Schizosaccharomyces pombe.

Shim YS et al. Mol Cell Biol 2000 Dec;20(23):8958-68

PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8

PMID:1427071 - Fission yeast genes involved in coupling mitosis to completion of DNA replication.

Enoch T et al. Genes Dev 1992 Nov;6(11):2035-46

PMID:18414064 - Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle.

Kato H et al. Cell Cycle 2008 Apr 15;7(8):1085-96

PMID:19723888 - Inhibition of type I histone deacetylase increases resistance of checkpoint-deficient cells to genotoxic agents through mitotic delay.

Alao JP et al. Mol Cancer Ther 2009 Sep;8(9):2606-15

PMID:12871901 - The Schizosaccharomyces pombe cdt2(+) gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication.

Yoshida SH et al. Genetics 2003 Jul;164(3):881-93

PMID:9398669 - A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes.

Yamaguchi S et al. Mol Biol Cell 1997 Dec;8(12):2475-86

PMID:10683155 - A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints.

Griffiths D et al. J Cell Sci 2000 Mar;113 ( Pt 6):1075-88

PMID:20816984 - A structural hinge in eukaryotic MutY homologues mediates catalytic activity and Rad9-Rad1-Hus1 checkpoint complex interactions.

Luncsford PJ et al. J Mol Biol 2010 Oct 29;403(3):351-70

PMID:9828139 - RAD1, a human structural homolog of the Schizosaccharomyces pombe RAD1 cell cycle checkpoint gene.

Marathi UK et al. Genomics 1998 Dec 01;54(2):344-7

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:20505337 - Roles of the checkpoint sensor clamp Rad9-Rad1-Hus1 (911)-complex and the clamp loaders Rad17-RFC and Ctf18-RFC in Schizosaccharomyces pombe telomere maintenance.

Khair L et al. Cell Cycle 2010 Jun 01;9(11):2237-48

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.

Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62

PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.

Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595

PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82

PMID:21493688 - Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.

Guarino E et al. Nucleic Acids Res 2011 Aug;39(14):5978-90

PMID:12032307 - A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway.

Marchetti MA et al. Proc Natl Acad Sci U S A 2002 May 28;99(11):7472-7